ID A0A418YFM2_9GAMM Unreviewed; 756 AA.
AC A0A418YFM2;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 13-SEP-2023, entry version 18.
DE SubName: Full=YgiQ family radical SAM protein {ECO:0000313|EMBL:RJG48099.1};
GN ORFNames=D1Z90_08460 {ECO:0000313|EMBL:RJG48099.1};
OS Motilimonas pumila.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC Alteromonadales genera incertae sedis; Motilimonas.
OX NCBI_TaxID=2303987 {ECO:0000313|EMBL:RJG48099.1, ECO:0000313|Proteomes:UP000283255};
RN [1] {ECO:0000313|EMBL:RJG48099.1, ECO:0000313|Proteomes:UP000283255}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PLHSC7-2 {ECO:0000313|EMBL:RJG48099.1,
RC ECO:0000313|Proteomes:UP000283255};
RA Wang F.;
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RJG48099.1, ECO:0000313|Proteomes:UP000283255}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PLHSC7-2 {ECO:0000313|EMBL:RJG48099.1,
RC ECO:0000313|Proteomes:UP000283255};
RA Wang F.-Q., Ren L.-H., Lin Y.-W., Sun G.-H., Du Z.-J., Zhao J.-X.,
RA Liu X.-J., Liu L.-J.;
RT "Motilimonas pumilus sp. nov., isolated from the gut of sea cucumber
RT (Apostichopus japonicus).";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01251};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|HAMAP-Rule:MF_01251};
CC -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000256|HAMAP-
CC Rule:MF_01251}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RJG48099.1}.
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DR EMBL; QZCH01000009; RJG48099.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A418YFM2; -.
DR OrthoDB; 9803479at2; -.
DR Proteomes; UP000283255; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR HAMAP; MF_01251; UPF0313; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR022946; UPF0313.
DR InterPro; IPR024560; UPF0313_C.
DR InterPro; IPR013704; UPF0313_N.
DR NCBIfam; TIGR03904; SAM_YgiQ; 1.
DR PANTHER; PTHR32331; UPF0313 PROTEIN YGIQ; 1.
DR PANTHER; PTHR32331:SF0; UPF0313 PROTEIN YGIQ; 1.
DR Pfam; PF11842; DUF3362; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF08497; Radical_SAM_N; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01069; UPF0313; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|HAMAP-
KW Rule:MF_01251};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01251}; Reference proteome {ECO:0000313|Proteomes:UP000283255};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01251}.
FT DOMAIN 362..639
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REGION 678..756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 741..756
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 376
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 380
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 383
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
SQ SEQUENCE 756 AA; 85119 MW; 5AFD7030C4753444 CRC64;
MYPAIKKIYD YKKYWAECFG PAPFLPTSRK EMKKLGWDSC DIILVTGDAY VDHPSFGMAV
IGRLLESQGF RVGIIAQPAW QDKTAFTELG EPNLFFGITA GNMDSMINRY TSDRKLRHDD
AYTPNNEGGK RPDRAVLVYS QRCREAYKSV PIVLGGIEAS LRRIAHYDYW SDKIRRSVLA
DAKADILLFG NAERALVDVA HGFAEGKTIE DMQHIRGTAV LRKTLPDHFK VVDSSRIDKP
GKAMVMQNPY QETSACATEK KAEPAKVVRM QTSRHDANNT AVRLPSFEKL KNDRILYAHA
SRVMHLETNP YSGRALVQKH ADRELWVNQA PIPLTTEEMD YVFGLPYARV PHPRYGKAKI
PAYDMIKTSV NIMRGCFGGC SFCSITEHEG RIIQNRSKQS ILDEMADIRD KVPGFTGTIS
DLGGPTANMY RLGCSDPKAE INCRRPSCVF PKICEKLNTD HKHTVDLYRS ARKVKGIKKV
MIASGVRYDL AIESPEYVKE LVTHHVGGYL KIAPEHTEKD TLDQMMKPGM GAYDKFKEMF
EKFSAMAGKK QYLIPYFIAA HPGCTDEDML NLAAWLKSND FQCDQVQNFY PSPMCNATAM
YHAETSPLTR VKYKKRKELF VAKGDKQRRL HKAFLRYHDP ANWPLLREAL TDMGKAHLIG
DKPHCIVPSA DDERAMNMRG TRRQSGRHGT KRFATKHPNQ PDIRKGGTAK AGAKGKHKPS
SNQKAGAKAA SPYQKPYGSK GKPKAKGKPK STRQGR
//
