ID A0A420F5G9_9ACTN Unreviewed; 1216 AA.
AC A0A420F5G9;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:RKF28174.1};
GN ORFNames=D7I43_07530 {ECO:0000313|EMBL:RKF28174.1};
OS Micromonospora globbae.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=1894969 {ECO:0000313|EMBL:RKF28174.1, ECO:0000313|Proteomes:UP000285744};
RN [1] {ECO:0000313|EMBL:RKF28174.1, ECO:0000313|Proteomes:UP000285744}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WPS1-2 {ECO:0000313|EMBL:RKF28174.1,
RC ECO:0000313|Proteomes:UP000285744};
RX PubMed=29458491; DOI=.1099/ijsem.0.002625;
RA Kuncharoen N., Pittayakhajonwut P., Tanasupawat S.;
RT "Micromonospora globbae sp. nov., an endophytic actinomycete isolated from
RT roots of Globba winitii C. H. Wright.";
RL Int. J. Syst. Evol. Microbiol. 68:1073-1077(2018).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKF28174.1}.
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DR EMBL; RAQQ01000004; RKF28174.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A420F5G9; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000285744; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000285744}.
FT DOMAIN 681..842
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 860..1017
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1216 AA; 133228 MW; 43A02EDFE3FF72E1 CRC64;
MLTGLFSAAL ADPGLARARD LARSGAAQVD GLDITAPPAL RPFAVAAVAA DQGGAGADGG
PAGGAGRPVL AVTATTREAD DLAAALGSLL PSEQVAVYPS WETLPHERLS PRSDTVGRRL
AVLRRLAHPE AADAHGRTGP LRVVVAPVRS LLQPQLKGLG DLEPVQLAPG AEADLEEVAR
RLTDMAYARV DLVTKRGEFA VRGGILDIFP PTDEHPSRIE FWGDEVEEIR TFAVADQRTI
EQVDRLWAPP CRELLLTPDV RRRAAALAER HPELAEILDK LAEGIPVEGM ESLVPALLGS
ESLELLLDVM PAGTHVLLCD PERIRTRAHD LVRTSEEFLQ ASWAAAAVGG QAPVDLGAAA
FRSLAEVRAA AAKLGQPWWT LSPFGLAAEG EAAPERQPWE DEPEEVSVTP DDAIAVTLAA
QPAPLYHGET ARVVDDLKRW SGEGWSIALV FEGHGPAQRA VEVLRDAGLG ARLTEQVPDA
PAPGELLVTC GSLSAGFVDE ASRFVLLTGN DITGGRGAST RDMRRMPSRR RNTIDPLELK
AGDYVVHEQH GIGRYIELVQ RTVNGASREY LVIEYAPSKR GQPGDRLFVP TDQLDQLSRY
VGGEQPTLHK MGGSDWQKSK ARARKAVREI AAQLIQLYAA RKASKGHSFG PDTPWQRELE
DAFPWQETPD QLAAIEEVKR DMEQSIPMDR LICGDVGYGK TEIAVRAAFK AVQDGKQVAV
LVPTTLLVQQ HYNTFAERMS QFPVTIRQLS RFQTPKEAEQ TLAMAADGTA DIVIGTHRLL
QSATRFKQLG LVIVDEEQRF GVEHKEHLKT MRASVDVLSM SATPIPRTLE MAITGIREMS
TIATPPEERH PVLTAVGAYD DRQVAAAIHR ELLRDGQVFY VHNRVESIER AARRIRELVP
EARVAVAHGQ MGEDALEKVM VGFWEKEFDV LVCTTIVESG IDIPNANTLI VERADLLGLA
QLHQIRGRVG RGRERAYAYF LYPPDKPLTE QAHERLATIA QHTELGAGMY VAMKDLEIRG
AGNLLGGEQS GHIEGVGFDL YVRMVGEAVQ AFKGEHAEEE TEVKVDLPVD AHLPHDYIGV
ERLRLEMYRK LAEARDEERL REVVAEMTDR YGEPPAPVQN LVAVARFRLL ARRYGLTDVS
MQGRHIRFSP LPLPDSKQMR LKRYHPDAVY KQATDQVSVP RPSTRRVGGE PLRDQALLEW
CAQLLTDVLG EPALVG
//
