ID A0A421JKA8_9ASCO Unreviewed; 835 AA.
AC A0A421JKA8;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN ORFNames=JA1_004239 {ECO:0000313|EMBL:RLV90925.1};
OS Spathaspora sp. JA1.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Spathaspora.
OX NCBI_TaxID=2028339 {ECO:0000313|EMBL:RLV90925.1, ECO:0000313|Proteomes:UP000285775};
RN [1] {ECO:0000313|EMBL:RLV90925.1, ECO:0000313|Proteomes:UP000285775}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JA1 {ECO:0000313|EMBL:RLV90925.1,
RC ECO:0000313|Proteomes:UP000285775};
RA Formighieri E.F., Steindorff A.S., Trichez D., Almeida J.R.;
RT "Physiological and comparative genomic analysis of newly identified yeasts
RT Spathaspora sp. JA1 and Meyerozyma sp. JA9 reveals insights into xylitol
RT production.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC {ECO:0000256|ARBA:ARBA00006637}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLV90925.1}.
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DR EMBL; NSGQ01000043; RLV90925.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A421JKA8; -.
DR STRING; 2028339.A0A421JKA8; -.
DR Proteomes; UP000285775; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation at RNA polymerase II promoter; IEA:InterPro.
DR CDD; cd18029; DEXHc_XPB; 1.
DR CDD; cd18789; SF2_C_XPB; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR032438; ERCC3_RAD25_C.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001161; XPB/Ssl2.
DR InterPro; IPR032830; XPB/Ssl2_N.
DR NCBIfam; TIGR00603; rad25; 1.
DR PANTHER; PTHR11274:SF0; GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB; 1.
DR PANTHER; PTHR11274; RAD25/XP-B DNA REPAIR HELICASE; 1.
DR Pfam; PF16203; ERCC3_RAD25_C; 1.
DR Pfam; PF13625; Helicase_C_3; 1.
DR Pfam; PF04851; ResIII; 1.
DR PRINTS; PR00851; XRODRMPGMNTB.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:RLV90925.1};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:RLV90925.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022806,
KW ECO:0000313|EMBL:RLV90925.1}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000285775}.
FT DOMAIN 362..524
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 578..732
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 812..835
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..50
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 835 AA; 94953 MW; 519B1FE0322A7E73 CRC64;
MASSRRTGQV NYAELNNGND DDYLSDDKIS DEDDYGTKPK KRKMKNQTVD NQVKRKQVQS
TVDYIRNEVD DTNFSDEELI ELTPEIPADY VPDAVSRAFG KADFSYLKLK PDHFSRPIWI
SPNDGRIILE SFSPLAEQAQ DFLITIAEPI SRPSHIHEYR ITAYSLYAAV SVGLETDDII
SVLNRLSKVP VAESIINFIK GATISYGKVK LVLKHNRYFV ESTQADILQM LLKDPVIGPL
RIQSSENTVT SNGLVQSAPP TNDIVIPGTK KTDQSNTEAE DIFASVVGTK EDDENDDDLD
TVHSFEISND SVEIVKKRCQ EIEYPVLEEY DFRNDSRNAD LEIDLKPSTQ IRPYQEKSLS
KMFGNGRARS GIIVLPCGAG KTLVGITAAC TIRKSVIVLC TSSVSVMQWR QQFLQWCTIQ
PENVAVFTSE NKEMFTSDSG LVVSTYSMVA NTRNRSHDSQ KVMDFLKSRE WGFIILDEVH
VVPAAMFRRV VTTIAAHAKL GLTATLVRED DKIDDLNFLI GPKLYEANWM DLASKGHIAN
VQCAEVWCPM TSEFYQEYLR ESARKRMLLY IMNPTKFQAC QFLIHYHEKR GDKIIVFSDN
VYALQEYALK LGKPFIYGST PQSERMKILQ NFQHNDEINT IFLSKVGDTS IDLPEATCLI
QISSHYGSRR QEAQRLGRIL RAKRRNDEGF NAFFYSLVSK DTQEMYYSTK RQAFLVDQGY
AFKVITHLSG MEQLNDLAYS SARERRELLQ QVLLKNEDAA GLEIGDDVDT NFISKERRMK
YEKEQANRTA GSLAGLAGGE DMAYIEYSKN KNKELREQSH HPLIQKMYYP KQGKK
//
