ID A0A421JMD6_9ASCO Unreviewed; 1446 AA.
AC A0A421JMD6;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=JA1_003508 {ECO:0000313|EMBL:RLV91968.1};
OS Spathaspora sp. JA1.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Spathaspora.
OX NCBI_TaxID=2028339 {ECO:0000313|EMBL:RLV91968.1, ECO:0000313|Proteomes:UP000285775};
RN [1] {ECO:0000313|EMBL:RLV91968.1, ECO:0000313|Proteomes:UP000285775}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JA1 {ECO:0000313|EMBL:RLV91968.1,
RC ECO:0000313|Proteomes:UP000285775};
RA Formighieri E.F., Steindorff A.S., Trichez D., Almeida J.R.;
RT "Physiological and comparative genomic analysis of newly identified yeasts
RT Spathaspora sp. JA1 and Meyerozyma sp. JA9 reveals insights into xylitol
RT production.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLV91968.1}.
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DR EMBL; NSGQ01000029; RLV91968.1; -; Genomic_DNA.
DR STRING; 2028339.A0A421JMD6; -.
DR Proteomes; UP000285775; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02736; RNAP_III_Rpc1_C; 1.
DR CDD; cd02583; RNAP_III_RPC1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR035698; RNAP_III_Rpc1_C.
DR InterPro; IPR035697; RNAP_III_RPC1_N.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF32; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000285775};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 253..564
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
SQ SEQUENCE 1446 AA; 161878 MW; 2E29813AAE1E96E7 CRC64;
MKEVVVDIAP KCIKGLEFSA LSAKDIVAQS EVEVQTRDLY DLEKGRTAKV LGALDTRMGI
SSNSSECNTC HGNLASCHGH FGHIRLALPV FHVGYFKSII QVLQCICKNC SGILLDEQSR
RTFLNDLRKP NIDNLRRMKI LKKILDQCKK QRRCVKCNHV NGVVKKAASG GGPAALKIVH
DTFRWIGKKA TPEKELWDQE FNLVFDRNPE LEKFIKRVNE DLNPLKTLNL FKQISPSDCE
LIGINSAKGG RPEMYIWRYL PAPPVCIRPS VMMDAQSNED DLTVKLTEIV WTSQLIRAGI
EKGISINNLM EQWDYLQLTV AMYINSDSAN PALLPNAGSS ASKASAKPIR GFCQRLKGKQ
GRFRGNLSGK RVDFSGRTVI SPDPNLKIDE VAVPDRVAKV LTYPEKCTRY NRVKLQQLVR
NGPDIHPGAN YVMKQNELAK RNLRFGNREM LAKNLAIGDI VERHIEDGDV VLFNRQPSLH
RLSILSHYAK IRPWRTFRLN ECVCTPYNAD FDGDEMNLHV PQTEEARAEA INLMGVKNNL
LTPKSGEPII AATQDFITGS YLISHKDSFY DRATFVQLLS MMSDADIQFD IPPPTIFKPV
MLWTGKQVFS LLIKPNKRSK VVINLDAKNK TFIPPTIKGL PSEMSPNDGF VIIRGSQILS
GLMDKSTLGD GKKHSVFYTI LRDYGPQEAA NAMNRMAKLC ARYLGNRGFS IGINDVTPEL
HLKQKKELMV EQAYLKCDEL IDLYNRGKLE TQPGCNEEQT LEAKIGGLLS KVREEVGEEC
IRELDSLNAP LIMATCGSKG STLNVSQMVA VVGQQIISGH RVPDGFQDRS LPHFMKNSKT
PQSKGFVRNS FYSGLTPPEF LFHAISGREG LVDTAVKTAE TGYMSRRLMK SLEDLSAQYD
NTVRNSSNGI VQFTYGGDGL DPYDMEGDAR PVNFNRQWDH AFNLTYNVKH PGLLPFEINE
LVNKILSPLE LKLIRYDNLG KELVAEDQSK IEYIDQKDAE REFYQSCRDF MNNKAEKLAR
LREAKGLAAH YTEGNSTDIE EDPIKLNSIN QLLKISRKTV EKFLKQCLYK YSRARVEPGT
AVGAIGAQSI GEPGTQMTLK TFHFAGVASM NVTLGVPRIK EIINASKTIS TPIINAVLVN
DDDEIAARVV KGRIEKTLLE DVAFSIEDVY KDNLAYLSIK IDLETIEKLQ LELTIENIVL
AIQNAPKLKI IAGDVSVSGK DRINVLVNLR EKTDSIKSET NKNALFFRMQ QLKRALPSIC
IKGLSNIFRA VINIRDDSKK ELLVEGYGLK QVMSTDGVVG TKTTTNHVLE VFEILGIEAA
RSSIIKEIDY TMSNHGMSVD PRHIQLLGDV MTYKGEVLGI TRFGLSKMRD SVLQLASFEK
TTDHLFDAAF FMKNDKIEGV SECIILGQIM SIGTGSFKLV KSSNYDKKVL KRKNTLFEST
CQPIAC
//
