ID A0A423PGV3_9GAMM Unreviewed; 305 AA.
AC A0A423PGV3;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=Tyrosine recombinase XerD {ECO:0000256|ARBA:ARBA00015810, ECO:0000256|HAMAP-Rule:MF_01807};
GN Name=xerD {ECO:0000256|HAMAP-Rule:MF_01807,
GN ECO:0000313|EMBL:ROO24706.1};
GN ORFNames=SAOR_14430 {ECO:0000313|EMBL:ROO24706.1};
OS Salinisphaera orenii MK-B5.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Salinisphaerales;
OC Salinisphaeraceae; Salinisphaera.
OX NCBI_TaxID=856730 {ECO:0000313|EMBL:ROO24706.1, ECO:0000313|Proteomes:UP000283993};
RN [1] {ECO:0000313|EMBL:ROO24706.1, ECO:0000313|Proteomes:UP000283993}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MK-B5 {ECO:0000313|EMBL:ROO24706.1,
RC ECO:0000313|Proteomes:UP000283993};
RA Lai Q., Li C., Shao Z.;
RT "Salinisphaera orenii MK-B5 Genome Sequencing.";
RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. The XerC-
CC XerD complex is essential to convert dimers of the bacterial chromosome
CC into monomers to permit their segregation at cell division. It also
CC contributes to the segregational stability of plasmids.
CC {ECO:0000256|HAMAP-Rule:MF_01807}.
CC -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC molecules of XerC and two molecules of XerD. {ECO:0000256|HAMAP-
CC Rule:MF_01807}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_01807}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerD subfamily.
CC {ECO:0000256|ARBA:ARBA00010450, ECO:0000256|HAMAP-Rule:MF_01807}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ROO24706.1}.
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DR EMBL; AYKH01000041; ROO24706.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A423PGV3; -.
DR Proteomes; UP000283993; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:DNA transposition; IEA:UniProtKB-UniRule.
DR CDD; cd00798; INT_XerDC_C; 1.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; Intergrase catalytic core; 1.
DR HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR HAMAP; MF_01807; Recomb_XerD; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR011932; Recomb_XerD.
DR InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR NCBIfam; TIGR02225; recomb_XerD; 1.
DR PANTHER; PTHR30349; PHAGE INTEGRASE-RELATED; 1.
DR PANTHER; PTHR30349:SF87; PROPHAGE INTEGRASE INTD-RELATED; 1.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cell cycle {ECO:0000256|ARBA:ARBA00023306, ECO:0000256|HAMAP-
KW Rule:MF_01807};
KW Cell division {ECO:0000256|ARBA:ARBA00022618, ECO:0000256|HAMAP-
KW Rule:MF_01807};
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829, ECO:0000256|HAMAP-
KW Rule:MF_01807};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01807};
KW DNA integration {ECO:0000256|ARBA:ARBA00022908, ECO:0000256|HAMAP-
KW Rule:MF_01807};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_01807};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_01807}; Reference proteome {ECO:0000313|Proteomes:UP000283993}.
FT DOMAIN 9..95
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000259|PROSITE:PS51900"
FT DOMAIN 116..299
FT /note="Tyr recombinase"
FT /evidence="ECO:0000259|PROSITE:PS51898"
FT ACT_SITE 156
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01807"
FT ACT_SITE 180
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01807"
FT ACT_SITE 251
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01807"
FT ACT_SITE 254
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01807"
FT ACT_SITE 277
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01807"
FT ACT_SITE 286
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01807"
SQ SEQUENCE 305 AA; 32989 MW; 90C254A4B13D031A CRC64;
MAAIDALPEP IAGEIECFLD GLWAERGLSD ATLAAYRNDL ARLAGWLVAQ GHDSLLAAGR
SELQAYLAAR NTPGSSPRSA ARLLSSLRQF YRYQRRAGAI DTDPTALIPS PAQGRRLPAT
LTEADVEALL AAPATDTDLG LRDRAMLELM YAAGLRVSEL VGASRAQVNL RQGVIRVTGK
GGRERLVPIG EAAVDWIERY LRQARPQLLD TARSDALFVT GRGGGMTRQN VWHRIRRHAA
SAGIRADVSP HGLRHAFATH LLNHGADLRA VQMLLGHADL STTQIYTHVA RARLAALHAS
HHPRG
//
