ID A0A426JGW0_9FLAO Unreviewed; 937 AA.
AC A0A426JGW0;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 22-FEB-2023, entry version 11.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN ORFNames=EIG84_12305 {ECO:0000313|EMBL:RRO12361.1};
OS Flavobacteriaceae bacterium 14752.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae.
OX NCBI_TaxID=2491711 {ECO:0000313|EMBL:RRO12361.1, ECO:0000313|Proteomes:UP000274596};
RN [1] {ECO:0000313|EMBL:RRO12361.1, ECO:0000313|Proteomes:UP000274596}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=14752 {ECO:0000313|EMBL:RRO12361.1,
RC ECO:0000313|Proteomes:UP000274596};
RA Yu Y.;
RT "Wangjiella luteus gen. nov., sp. nov., a novel bacterium of the family
RT Flavobacteriaceae isolated from a saline lake.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RRO12361.1}.
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DR EMBL; RRZV01000095; RRO12361.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A426JGW0; -.
DR OrthoDB; 9759819at2; -.
DR Proteomes; UP000274596; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd18032; DEXHc_RE_I_III_res; 1.
DR CDD; cd18799; SF2_C_EcoAI-like; 1.
DR Gene3D; 3.90.1570.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013670; EcoEI_R_C_dom.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR PANTHER; PTHR47396:SF1; ATP-DEPENDENT HELICASE IRC3-RELATED; 1.
DR PANTHER; PTHR47396; TYPE I RESTRICTION ENZYME ECOKI R PROTEIN; 1.
DR Pfam; PF08463; EcoEI_R_C; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Helicase {ECO:0000313|EMBL:RRO12361.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT DOMAIN 175..370
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 937 AA; 108564 MW; DD4B3D59B662968E CRC64;
MPADINQNPE QIARDKIDKM LLEAGWEVQS KSKVDLNAKK GVAVREYQTD VGPADYVLFV
DKKPVGIIEA KREDEGHRLT VVEDQSTNYA NAKLKYLNND PLPFVYESTG TITRFTDYRD
PKPRGRTVFW FHKPETIAKW LKKGKSLRER LLSMPDLNEM GLRPAQIVAI NNLEHSFKKN
RPKALIQMAT GAGKTFTAAT FVYRLLKHAD AKRILFLVDT KNLGEQAEQE FMTFQPNDDN
RKFTELYNVQ RLTSSYIASD SQVSISTIQR LYSILKGEEL EESAEQDNPN ESSWLKSKMS
EKKAIPVEYT PRVPIEQFDF IVIDEAHRSI YNLWKQVLDY FDAFLIGLTA TPDKRTFGFF
NENVVSQYTY EESVTDGVNV PYDVYTIETE ISQKGETVKA GWFVDRRDKL TRKKRWQQED
EDTEYKRNDL DKKVVNPSQI RNIIREYKRA LKTTIFPNRS DENGDYEVPK TLVFAKTDSH
ADDIIKIIRE EFDEGNDFCK KVTYKIEEDP KSVLNRFRNS YHPRIAVTVD MIATGTDVKP
LEVLLFMRDV KSVNYFEQMK GRGTRTINKD SLQLVSRTAN SKTHFVIVDA VGATKSKKTD
SRPLERKPSV AMKDLLGAIT MGVAEEDLFM SLANRLIRLE KQITDKEKDK ILEFSKGKNL
KQITKELITA FDKDAINDKA QPEIDKIPIE DRTPALVEET KKKAQEELIN EASKTFNGEF
NDYLENVRKE HDQIIDSINI DTVIKSEWDT NSVDKAKAIV KDFNEYLEAN KDEIKALSIF
YNQPYNRREI TFKMIKEVME KMKLEKPLLA PDYVWDAYTQ LEEVKSKQPK DELTALVSLI
RRACGIDTKL VAFDKTIDEN FKNWIFKQNA GKHNRFDKEQ MQWLRMVKDH IVSSYHLELE
DLDYTPFDNQ GGRGKMYQLF GNEMDNVIKE LNEVLVA
//
