ID A0A426VEN0_9BURK Unreviewed; 830 AA.
AC A0A426VEN0;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
DE AltName: Full=NHEJ DNA polymerase {ECO:0000256|ARBA:ARBA00029943};
GN Name=ligD {ECO:0000313|EMBL:RRS05352.1};
GN ORFNames=EIP75_06470 {ECO:0000313|EMBL:RRS05352.1};
OS Aquabacterium soli.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Aquabacterium.
OX NCBI_TaxID=2493092 {ECO:0000313|EMBL:RRS05352.1, ECO:0000313|Proteomes:UP000269265};
RN [1] {ECO:0000313|EMBL:RRS05352.1, ECO:0000313|Proteomes:UP000269265}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SJQ9 {ECO:0000313|EMBL:RRS05352.1,
RC ECO:0000313|Proteomes:UP000269265};
RA Sun L., Gao X., Chen W., Huang K.;
RT "The whole draft genome of Aquabacterium sp. SJQ9.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RRS05352.1}.
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DR EMBL; RSED01000004; RRS05352.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A426VEN0; -.
DR OrthoDB; 9802472at2; -.
DR Proteomes; UP000269265; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd07906; Adenylation_DNA_ligase_LigD_LigC; 1.
DR CDD; cd07971; OBF_DNA_ligase_LigD; 1.
DR CDD; cd04862; PaeLigD_Pol_like; 1.
DR Gene3D; 3.30.1490.70; -; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR014146; LigD_ligase_dom.
DR InterPro; IPR014144; LigD_PE_domain.
DR InterPro; IPR014145; LigD_pol_dom.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014143; NHEJ_ligase_prk.
DR InterPro; IPR033651; PaeLigD_Pol-like.
DR NCBIfam; TIGR02777; LigD_PE_dom; 1.
DR NCBIfam; TIGR02778; ligD_pol; 1.
DR NCBIfam; TIGR02779; NHEJ_ligase_lig; 1.
DR NCBIfam; TIGR02776; NHEJ_ligase_prk; 1.
DR PANTHER; PTHR42705; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR PANTHER; PTHR42705:SF2; MULTIFUNCTIONAL NON-HOMOLOGOUS END JOINING DNA REPAIR PROTEIN LIGD; 1.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF13298; LigD_N; 1.
DR Pfam; PF21686; LigD_Prim-Pol; 1.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:RRS05352.1};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000269265};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 304..404
FT /note="ATP-dependent DNA ligase family profile"
FT /evidence="ECO:0000259|PROSITE:PS50160"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 830 AA; 91482 MW; A5BF87E72C58EB8B CRC64;
MAKDDKLHHY RSKRDFSITS EPEGQVGKPG QALGFVIQKH AARRLHYDFR LELDGTLKSW
AVPKGPSLDP ADKRMAVHVE DHPLAYGGFE GVIPAGQYGA GTVIVWDRGE WVPLGDPAKG
YKDGKLKFEL RGHKLQGHWT LVRMRGRGQE RQEPWLLIKE RDEAARPASE YDVVEALPDS
VLKASQPTRK KAKAVHSESM PLTQAPQLAT LVNSVPSEGD WLYEIKFDGY RLMARVDQGT
VRLFTRNGHD WTSRLKRLAA ELGQLGLKSG WLDGEIVVIG SHGGTDFQAL QNAFDLVRTE
PIQYFVFDLL HVDGEDLRDQ PLKARRERLR ALIDGAAADA HPHVRYSEDF QVEPAHLLHT
ACAMRMEGVI GKRLDAPYRA GRSDAWIKLK CTQRQEFVIG GYTDPKGSRQ GLGALLLGTH
DEQGQLRYAG NVGSGFDAHA LRDVHKRLQA LATGKPAFVG LPGDVKGHWV KPTLVAEVSF
AEWTKEGRLR QAVFHGLRAD KPARAITRET AKPPPGDAAA LPARKATKRA STAKPGAQHH
SVAGVRISHA DRIIDPSTGI TKGELADYHA RAARRMLLHL AGRPVSLVRA PEGIQGELFF
QKHADRMSIP GIERLDPALD PGHAPLLAVP SREALVGAAQ MNVIELHTWN ALIDHIEQPD
RMTFDLDPGE GVAWAHVQEG AELVRVMLEE LSLRSFLKTS GGKGLHVVVP FKPSLGWDQV
KGLSKALVEH LASAIPDRFV SKSGPRNRVG KIFVDYLRNG RGATTASAWT ARARPGMGVS
VPVDWSELPE LSSGAHWTLR TVDERLTGDD PWAGYKRVKQ SLREALKAMG
//
