ID A0A429G4B5_9ACTN Unreviewed; 720 AA.
AC A0A429G4B5;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Anthranilate synthase {ECO:0000256|PIRNR:PIRNR036934};
DE EC=4.1.3.27 {ECO:0000256|PIRNR:PIRNR036934};
GN ORFNames=DMH08_10235 {ECO:0000313|EMBL:RSN68691.1};
OS Actinomadura sp. WAC 06369.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Thermomonosporaceae; Actinomadura.
OX NCBI_TaxID=2203193 {ECO:0000313|EMBL:RSN68691.1, ECO:0000313|Proteomes:UP000288610};
RN [1] {ECO:0000313|EMBL:RSN68691.1, ECO:0000313|Proteomes:UP000288610}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WAC 06369 {ECO:0000313|EMBL:RSN68691.1,
RC ECO:0000313|Proteomes:UP000288610};
RA Waglechner N., Wright G.D.;
RT "Evolution of GPA BGCs.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chorismate + L-glutamine = anthranilate + H(+) + L-glutamate +
CC pyruvate; Xref=Rhea:RHEA:21732, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16567, ChEBI:CHEBI:29748, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58359; EC=4.1.3.27;
CC Evidence={ECO:0000256|PIRNR:PIRNR036934};
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 1/5. {ECO:0000256|PIRNR:PIRNR036934}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RSN68691.1}.
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DR EMBL; QHJN01000027; RSN68691.1; -; Genomic_DNA.
DR RefSeq; WP_026406189.1; NZ_QHJN01000027.1.
DR AlphaFoldDB; A0A429G4B5; -.
DR UniPathway; UPA00035; UER00040.
DR Proteomes; UP000288610; Unassembled WGS sequence.
DR GO; GO:0004049; F:anthranilate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01743; GATase1_Anthranilate_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.60.120.10; Anthranilate synthase; 1.
DR InterPro; IPR005801; ADC_synthase.
DR InterPro; IPR019999; Anth_synth_I-like.
DR InterPro; IPR006805; Anth_synth_I_N.
DR InterPro; IPR015890; Chorismate_C.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR010112; TrpE-G_bact.
DR InterPro; IPR006221; TrpG/PapA_dom.
DR NCBIfam; TIGR01815; TrpE-clade3; 1.
DR NCBIfam; TIGR00566; trpG_papA; 1.
DR PANTHER; PTHR11236; AMINOBENZOATE/ANTHRANILATE SYNTHASE; 1.
DR PANTHER; PTHR11236:SF9; ANTHRANILATE SYNTHASE COMPONENT 1; 1.
DR Pfam; PF04715; Anth_synt_I_N; 1.
DR Pfam; PF00425; Chorismate_bind; 1.
DR Pfam; PF00117; GATase; 1.
DR PIRSF; PIRSF036934; TrpE-G; 1.
DR PRINTS; PR00097; ANTSNTHASEII.
DR PRINTS; PR00096; GATASE.
DR SUPFAM; SSF56322; ADC synthase; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 4: Predicted;
KW Amino-acid biosynthesis {ECO:0000256|PIRNR:PIRNR036934};
KW Aromatic amino acid biosynthesis {ECO:0000256|PIRNR:PIRNR036934};
KW Glutamine amidotransferase {ECO:0000256|PROSITE-ProRule:PRU00605};
KW Lyase {ECO:0000256|PIRNR:PIRNR036934, ECO:0000313|EMBL:RSN68691.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000288610};
KW Tryptophan biosynthesis {ECO:0000256|PIRNR:PIRNR036934}.
FT DOMAIN 39..203
FT /note="Anthranilate synthase component I N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04715"
FT DOMAIN 246..496
FT /note="Chorismate-utilising enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00425"
FT DOMAIN 534..706
FT /note="Glutamine amidotransferase"
FT /evidence="ECO:0000259|Pfam:PF00117"
FT REGION 218..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 507..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 511..530
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 609
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 696
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 698
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 720 AA; 78601 MW; 6193FD66DD0CAE3B CRC64;
METVSQQPTT GEFVTAGGVR VTRTAAPVDP ERKSDVLNAL VAAVGERRGG VLSSGMEYPG
RYSRWHMAYV DPCLEIVARG RTVAARALNG RGRVVLPAVA EVLRGTGEVR ADGPGECAVF
VPPTEEFFPE EERSRQPTVF TALRAVVDLF RCDDPHLGLY GAFGYDLSLQ FEPLRPRLER
PDDQRDLVLH LADEMVVVDR KRETSHRISY DFEVGGTATA GLPRDTEPTP PVVPAELPPQ
PVPGVYAQMV RDAKERFVRG DLFEVTPGHA MHGRCDSPAA FFERLRETNP APYEFLFNLG
DGEYLVGASP EMFVRVTGDR VETCPIAGTI KRGGDALQDA EQIRTILASA KDESELTMCT
DVDRNDKSRV CVPGSVRVLG RRQIEMYSRL IHTVDHIEGR LRPEFDALDA FLTHMWAVTV
TGAPKTWAMQ FIEDHEDSPR RWYGGAVGFV GFDGSMNTGL TLRTAQIRDG VATVRAGATL
LYDSDPDEEE RETHLKASAL LGALAATARD GAEPEADREP EPAPERPGDG LKVLLVDHED
SFVNTLADYF RQHGAGVVTL RHGFPARLLD EHAPDLVVLS PGPGRPDDFA TRDLLDALDA
RGLPVFGVCL GLQAMVEHAG GSLDLLPEPS HGKPGQVKVT GGGLFEGLPD EFTAARYHSL
HATPDRVRGF QVTATTGDVV MAIENAERRR WAVQFHPESI LTAAGGAGHR IVANVLRLCR
//
