ID A0A429NA03_9ACTN Unreviewed; 794 AA.
AC A0A429NA03;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE SubName: Full=Glycoside hydrolase family 65 protein {ECO:0000313|EMBL:RSS03039.1};
GN ORFNames=EF917_13815 {ECO:0000313|EMBL:RSS03039.1};
OS Streptomyces sp. WAC00469.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2487415 {ECO:0000313|EMBL:RSS03039.1, ECO:0000313|Proteomes:UP000269080};
RN [1] {ECO:0000313|EMBL:RSS03039.1, ECO:0000313|Proteomes:UP000269080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WAC00469 {ECO:0000313|EMBL:RSS03039.1,
RC ECO:0000313|Proteomes:UP000269080};
RA Culp E., Yim G., Waglechner N., Wang W., Pawlowski A.C., Wright G.D.;
RT "Unmasking the antibiotic production potential of Actinomycetes through
RT CRISPR/Cas9 inactivation of ubiquitous gene clusters.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 65 family.
CC {ECO:0000256|ARBA:ARBA00006768}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RSS03039.1}.
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DR EMBL; RPRX01000038; RSS03039.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A429NA03; -.
DR OrthoDB; 9816160at2; -.
DR Proteomes; UP000269080; Unassembled WGS sequence.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.70.98.40; Glycoside hydrolase, family 65, N-terminal domain; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR005194; Glyco_hydro_65_C.
DR InterPro; IPR005195; Glyco_hydro_65_M.
DR InterPro; IPR005196; Glyco_hydro_65_N.
DR InterPro; IPR037018; Glyco_hydro_65_N_sf.
DR InterPro; IPR017045; Malt_Pase/Glycosyl_Hdrlase.
DR PANTHER; PTHR11051; GLYCOSYL HYDROLASE-RELATED; 1.
DR PANTHER; PTHR11051:SF8; PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE; 1.
DR Pfam; PF03633; Glyco_hydro_65C; 1.
DR Pfam; PF03632; Glyco_hydro_65m; 1.
DR Pfam; PF03636; Glyco_hydro_65N; 1.
DR PIRSF; PIRSF036289; Glycosyl_hydrolase_malt_phosph; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000313|EMBL:RSS03039.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000269080};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 11..261
FT /note="Glycoside hydrolase family 65 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03636"
FT DOMAIN 314..708
FT /note="Glycoside hydrolase family 65 central catalytic"
FT /evidence="ECO:0000259|Pfam:PF03632"
FT DOMAIN 718..782
FT /note="Glycoside hydrolase family 65 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03633"
FT ACT_SITE 489
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036289-50"
FT BINDING 350..351
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
FT BINDING 609..610
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
SQ SEQUENCE 794 AA; 88933 MW; BB2B5229D871C7EB CRC64;
MTSITAGTWE YDGYDPAQER LREALCTLGN GRFATRGALP ECAADDVHYP GTYVAGCYNR
LVSRVADRQV ENEDLVNLPN WLPVRFRRPG GSWLTPDTST VLGHRQTLHL EAGVLERRTG
YDLGDGHTLM VRQLRIVHMA DPYLAALRTE LTVRGGPVDL EVEAAIDGGV TNAGVPRYRD
LDGRHLTDVD TGTLAPGTVW LHCRTRTSEI GIGVASRLTA HGDALVHTRH EQQQAIQTVH
LRLLADRPVT LDKIVALHTS HDPAISDPLR AATDRVLVAP HFDDLLTAHR AAWDGLWRRA
RIDVPGEPGR ILHLHLFHVL QTLSPNTADL DVGVPARGLH GEAYRGHVFW DELFVLPYLN
LHLPEVSRAL LQYRHRRLGA ACAAARALGR RGAMYPWQSG SDGREETQRL HLNPRSGRWL
PDHTHLQRHV GSAIAYNIWQ YCEASGDTEF LQTQGAEMLV QIARFWADSA TWDPHLGRYR
IRGVVGPDEY HDAYPGAERP GLDDNTYTNV TAAWVLARTL DTLRALPEPR RSELVERLEM
DDDEPLRWEE VSRTLYVPTH DGVISQFDGY GELEELDWAG YRTRYGDIRR LDRILEAEGD
TPNRYKASKQ ADLLMLGYLF SPAELQALFR RLGRPVDEEL WQRCVDYYLA RTSHGSTLSG
LVHGWILTRA RRAEAWSFVQ EALRGDIADL QGGTTGEGIH LGAMAGTLDL VQRGLTGLET
RDGAIWLDPV PLPELSFYGF RLRYRGHWGV GLRLEHGRLE IAVPPSGRAP IEVRLPDGTV
HVQPGEKCSL VLPD
//
