ID A0A429ZK93_9ENTE Unreviewed; 746 AA.
AC A0A429ZK93;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN ORFNames=CBF35_10465 {ECO:0000313|EMBL:RST94130.1};
OS Vagococcus salmoninarum.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus.
OX NCBI_TaxID=2739 {ECO:0000313|EMBL:RST94130.1, ECO:0000313|Proteomes:UP000287239};
RN [1] {ECO:0000313|EMBL:RST94130.1, ECO:0000313|Proteomes:UP000287239}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCFB 2777 {ECO:0000313|EMBL:RST94130.1,
RC ECO:0000313|Proteomes:UP000287239};
RA Gulvik C.A.;
RT "Vagococcus spp. assemblies.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RST94130.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; NGJU01000016; RST94130.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A429ZK93; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000287239; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000287239}.
FT DOMAIN 9..288
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 289..572
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 746 AA; 84650 MW; 1C1784612CFFCA65 CRC64;
MSTRNNLVNG MNPMQKEAVL QTEGPLLIMA GAGSGKTRVL THRIAYLITE KEVNPWNILA
ITFTNKAAKE MKERVNSLLT TGGNDVWVST FHSMCVRMLR RDVDQIGYNS NFTIIDGSEQ
LTLMKRILKE QNIDPKQHDP RSIIGAISNA KNELQTPKIY EAKNQGFVEA IVAKCYHAYQ
KELRHNQCMD FDDLIMLTIR LFEDSPETLQ YYQNKFHYIH VDEYQDTNHA QYTLVNMLAD
RFKNLCVVGD ADQSIYGWRG ADMENILDFE KDYPTAKVVL LEQNYRSTKN ILTAANHVIG
NNSKRKDKKL WTDNLEGEKI TYYRGETERG ETQYIVQSIQ KEILENQRNY GDFAVLYRTN
ALSRVMEEML LKSNIPYKMV GGHKFYDRKE IKDILAYLNV IANPMDSLSY ARVVNEPKRG
IGATSIEKLR DFANIHEWSL LEASLNVDLA NISGKAGKEL ANFAIMMEDL QQMVPFLTIT
ELVEETLKRS GYEADLKRQN TLESQNRLEN LEEFLTVTQA FDKQQAAAEE ELADPTEKLT
IFLNDLALVA DIDSLEEETS QVTLMTLHAA KGLEFPVVFL LGLEEGIFPL SRALMEESEL
EEERRLAYVG ITRAEQKLFI SNAFSRTLYG RTQYNRPSRF ITEINQELLM NEGSATALQN
RQSYTTGASV VRPTAPQAQA VKTSAKVATG GDKEAWRSGD KVNHKKWGVG TVVKVSGSAK
DIELDIAFPA QGIKRLLAAF APIEKM
//
