ID A0A429ZUU8_9ENTE Unreviewed; 518 AA.
AC A0A429ZUU8;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 11.
DE RecName: Full=Alpha-glycerophosphate oxidase {ECO:0000256|ARBA:ARBA00021658};
DE EC=1.1.3.21 {ECO:0000256|ARBA:ARBA00013104};
DE AltName: Full=Glycerol-3-phosphate oxidase {ECO:0000256|ARBA:ARBA00032349};
GN ORFNames=CBF35_02095 {ECO:0000313|EMBL:RST97481.1};
OS Vagococcus salmoninarum.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus.
OX NCBI_TaxID=2739 {ECO:0000313|EMBL:RST97481.1, ECO:0000313|Proteomes:UP000287239};
RN [1] {ECO:0000313|EMBL:RST97481.1, ECO:0000313|Proteomes:UP000287239}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCFB 2777 {ECO:0000313|EMBL:RST97481.1,
RC ECO:0000313|Proteomes:UP000287239};
RA Gulvik C.A.;
RT "Vagococcus spp. assemblies.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=O2 + sn-glycerol 3-phosphate = dihydroxyacetone phosphate +
CC H2O2; Xref=Rhea:RHEA:18369, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57642; EC=1.1.3.21;
CC Evidence={ECO:0000256|ARBA:ARBA00000275};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007330}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RST97481.1}.
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DR EMBL; NGJU01000002; RST97481.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A429ZUU8; -.
DR OrthoDB; 9766796at2; -.
DR Proteomes; UP000287239; Unassembled WGS sequence.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase (quinone) activity; IEA:InterPro.
DR GO; GO:0004369; F:glycerol-3-phosphate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR Gene3D; 1.10.8.870; Alpha-glycerophosphate oxidase, cap domain; 1.
DR Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR031656; DAO_C.
DR InterPro; IPR038299; DAO_C_sf.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000447; G3P_DH_FAD-dep.
DR PANTHER; PTHR11985:SF37; AEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE; 1.
DR PANTHER; PTHR11985; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; 1.
DR Pfam; PF01266; DAO; 1.
DR Pfam; PF16901; DAO_C; 1.
DR PRINTS; PR01001; FADG3PDH.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS00977; FAD_G3PDH_1; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000287239}.
FT DOMAIN 21..322
FT /note="FAD dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF01266"
FT DOMAIN 378..502
FT /note="Alpha-glycerophosphate oxidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16901"
SQ SEQUENCE 518 AA; 58210 MW; 4289815699F1A2D9 CRC64;
MNLSRETRQK HLRIMTETPL DLLVIGGGIT GAGITIDGIS RGLGVALIEM GDFASGATSA
SHKLLRGEIS TNNGERKGLL ENAPHLTKAI KVLTPIFKQQ ELSLLKSAVA LKAYDFLSQT
DKDLAFFTLN RSETLKREPL LKKENLKSSS IYTEYRTNDS RLTLEIIKKA VDFGAYVANY
TKVVKFLYNH QTGKIIGVRA IDGLTGEEIA IYAKRVVNAT GSWSGELSRL DKGTPLPNPS
LKEQHYIVLS AKALPVTNAL NFLSEITDQS YFVIPVGDKI YVGPHVTSLN QQTLAQQQLL
DALNDFFTMP PIELKSIESS WTEPSVQDSP SDSYSLSRSG LISVTLTELY TYRLQSKLVV
DRLLRSICKE YHRLFIKCRT KNLSVAGGDV GGSTGFHRYQ KEFIRIGVEK FHLSHGHASL
LVNRYGSNVI DLYAYLLNVP PHTNLADLDY VMLRYALEAE MCLHPLDFLK RRTDYFYFNK
EQAIAIADHV IDEMAKYYQW SKIYHEQIIT ETWLALAN
//
