ID A0A429ZXT7_9ENTE Unreviewed; 387 AA.
AC A0A429ZXT7;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=Hydroxymethylglutaryl-CoA synthase {ECO:0000313|EMBL:RST98714.1};
GN ORFNames=CBF37_06600 {ECO:0000313|EMBL:RST98714.1};
OS Vagococcus vulneris.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus.
OX NCBI_TaxID=1977869 {ECO:0000313|EMBL:RST98714.1, ECO:0000313|Proteomes:UP000287857};
RN [1] {ECO:0000313|EMBL:RST98714.1, ECO:0000313|Proteomes:UP000287857}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SS1995 {ECO:0000313|EMBL:RST98714.1,
RC ECO:0000313|Proteomes:UP000287857};
RA Gulvik C.A.;
RT "Vagococcus spp. assemblies.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000256|ARBA:ARBA00007061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RST98714.1}.
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DR EMBL; NGJS01000008; RST98714.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A429ZXT7; -.
DR OrthoDB; 9769523at2; -.
DR Proteomes; UP000287857; Unassembled WGS sequence.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:InterPro.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IEA:InterPro.
DR CDD; cd00827; init_cond_enzymes; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR011554; HMG_CoA_synthase_prok.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01835; HMG-CoA-S_prok; 1.
DR PANTHER; PTHR43323; 3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE; 1.
DR PANTHER; PTHR43323:SF2; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF08540; HMG_CoA_synt_C; 2.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000287857}.
FT DOMAIN 3..164
FT /note="Hydroxymethylglutaryl-coenzyme A synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01154"
FT DOMAIN 177..247
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT DOMAIN 264..385
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT ACT_SITE 78
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 110
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 232
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT BINDING 28
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 142
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 147
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 241
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 274
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
SQ SEQUENCE 387 AA; 43017 MW; 08A87FAC052D6B95 CRC64;
MAGIDKISFF TPNTYVNLEK LAIHRGIDPN KFLIGIGQTN MAVPTITQDT VSMGANAALS
ILSEEDRELI DLVIVGTESG VDQSKSCASF IHDILNIQPF AKSYEIKHAC YGATAGLMSA
IDYVQNNPDR KALVIGSDIA RYGLNSGGEV TQGAGAVAML ISNNPRILEF DTPSISLTKN
IFDFWRPNYS DVAFVDGKFS NEAYIEFFNT LWQEFSNKNN LLLEDIDAFC LHMPYTKMGQ
KALSPLLAEA KTEIRDSLLS QYELSTTYSR QIGNIYTGSL YLSLISLLDQ SSTLVSGDRI
AFFSYGSGAV AEMFSATLVK DYTDQLLAQH HQTMLNQRTS LTIEQYETIF SQELPKEDGL
YTFEQELDTA SIYLSAITDH QRIYTKK
//
