ID A0A430ALR6_9ENTE Unreviewed; 698 AA.
AC A0A430ALR6;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 13.
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
GN Name=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN ORFNames=CBF29_12455 {ECO:0000313|EMBL:RSU09016.1};
OS Vagococcus elongatus.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus.
OX NCBI_TaxID=180344 {ECO:0000313|EMBL:RSU09016.1, ECO:0000313|Proteomes:UP000287605};
RN [1] {ECO:0000313|EMBL:RSU09016.1, ECO:0000313|Proteomes:UP000287605}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 51432 {ECO:0000313|EMBL:RSU09016.1,
RC ECO:0000313|Proteomes:UP000287605};
RA Gulvik C.A.;
RT "Vagococcus spp. assemblies.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RSU09016.1}.
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DR EMBL; NGKA01000027; RSU09016.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A430ALR6; -.
DR OrthoDB; 9775440at2; -.
DR Proteomes; UP000287605; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01045; TRNASYNTHGB.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000287605}.
FT DOMAIN 585..681
FT /note="DALR anticodon binding"
FT /evidence="ECO:0000259|Pfam:PF05746"
FT COILED 368..395
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 698 AA; 79728 MW; 971A748D30C2744C CRC64;
MTKTYLLEIG LEEMPARVVT SSVNQLKEGM ANFLKDNRLS YDTIETYATP RRLALKVINL
AEKQTDVEEN AKGPAKRIAV DEDGNWTRAA EGFARGQGVS LEELYMKEVK GVEYVYADKF
ISGEAAEKVL TSALDILKAL HFPVTMRWGS FDTEFIRPIH WIVSLLDDQI IPLNFLNVTS
GNVSRGHRFL GTDTVIEHSK DYETNLREQF VIADAMERKA MIREQIREIA EKNHWHISLD
EKLLEEVNNL VEYPTAFAGN YAEKYLDIPE EALIISMKEH QRYFEVRDRD EKLLPYFIGV
RNGNDQYLDN VISGNEKVLV ARLEDAVFFY EEDQQVAIEE YVNRLRKVTF HEKIGSIYEK
MQRVASIAKL IGEQVKLTEE ELKDLERAAA IYKFDLMTSM VGEFPELQGV MGEKYALIKG
ERPAVAQAIR EHYLPSSSEG VLPVSNVGAV LAVADKLDTV TTFFNAGMVP TGSNDPYALR
RQTYGIIRIV EDKGWDYPIL RLQEEIAEII NDNPDKFGVQ LSTDNQKVFD FMKARLKQFL
SSDDIRHDVI EAVTNAKQSN LIKMIDAARI LKKHQQDSDY KSAMEALGRV TNLAKKGEEL
LQEDIHIPVD ESLFENEEEK ELFHAVNQLL TKVKKHEPED TYLALKDLAP LIEKYFDSTM
VMADDEGKRN NRLRQLMLIS DLASAFASID LLTIKNKE
//
