ID A0A430BL27_9SPHN Unreviewed; 1142 AA.
AC A0A430BL27;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN Name=addA {ECO:0000313|EMBL:RSU52354.1};
GN ORFNames=BRX43_05245 {ECO:0000313|EMBL:RSU52354.1};
OS Sphingomonas sp. S-NIH.Pt15_0812.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=1920129 {ECO:0000313|EMBL:RSU52354.1, ECO:0000313|Proteomes:UP000287772};
RN [1] {ECO:0000313|EMBL:RSU52354.1, ECO:0000313|Proteomes:UP000287772}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S-NIH.Pt15_0812 {ECO:0000313|EMBL:RSU52354.1,
RC ECO:0000313|Proteomes:UP000287772};
RA Johnson R.C., Deming C., Conlan S., Zellmer C., Michelin A., Lee-Lin S.-Q.,
RA Thomas P.J., Park M., Weingarten R.A., Less J., Dekker J.P., Frank K.M.,
RA Musser K.A., Mcquiston J.R., Henderson D.K., Lau A.F., Palmore T.N.,
RA Segre J.A.;
RT "Genomic and Epidemiologic Investigation to Identify Sphingomonas koreensis
RT Point Sources in an Indolent Hospital Outbreak.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RSU52354.1}.
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DR EMBL; QLJH01000005; RSU52354.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A430BL27; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000287772; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.160.800; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR014151; DNA_helicase_AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02784; addA_alphas; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000287772}.
FT DOMAIN 10..497
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 501..787
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 805..829
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 924..960
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1142 AA; 122235 MW; 7458DD3D2D866C33 CRC64;
MAVTAELRPL PRLKGEQAAA SDPHAHVWLS ASAGTGKTQV LSARVFRLLL KGVDPSAILC
LTFTKAGAAE MAHRISQRLA AWVRMADTDL ANDLYALGER ADPEQRARAR TLFAKVLDAP
GGGLRIQTIH GFCQGLLAAF PVEAGLAPGF RPLEAREEAV LAREALARML TDAEREGRGA
VVETIGALSL RLGEGGAEGF LIACARSLAA LEALPVGIGP YLRRQLDLPQ GDVGEAVAAE
CAGFDETALR RLAACNAAWG TKNGLARADA IAAWLARDPA DRAATLADLH AVWARADGEP
RSFAKGQAPQ EPDYAETASR FHGQCAALLS LATRAAYADL LGAGLSVGRD YARAYDRAKR
QVGGVDFDDL IRVTVSLLEQ PGVGEWVRYK LDQVTEHVLI DEAQDTNLNQ WRIVSAVADE
FFVGRGIYAP RTRTLFTVGD WKQAIFGFQG TSPVNFAWAE RRFGGRAQEA LGDDDWPEHE
RGLPFERLSL THSFRSTRGI LRFVDAAVAV VPEPALGAIG LMEDHASEVA GPGHVALWPP
VVAGGSEEDE ESWVDDAVRK LAGDVARAVR RWLDEGLVLG AKGRPLRPED VMILVKRRGD
LASLLVARLY AEGVPVAGVD RLRLNAPLAV QDLLAAVRFA LQPDDDLSLA SLLVSPLIGW
SQDELMHAAP RTHGSLWRHL TQTLSEDRIA PLRLILNRAD FTTPYQFLEE LLSGPIGGRR
KLLARLGAEA RDPIEELLSA ALTFEHMTTP SLQRFLDWFD RGDVEIVRDP SAPMDAVRVM
TAHGAKGLQA PLVILADATV DPTQSPRTTL RWSPEPGAAP LPVFPPRATE RGGPLDMVKA
EAEAAELEEH WRLFYVAATR AEERLVIAGA LGPKAKGVPP QASWYAAAAR GFDSLGVPAG
EGERVYEEGA ALAPRGGAAA MPVTEPTAPL PGWSRTPAPK ESRPSRPLAP SAMGEDLVAD
PPPTAAMRAA AERGRLLHAL FERLPMVVPA RRAGAAERWL ATAGAVADAT ERRQLTEAAL
SVIEDPRFAA LFGPDSLAEA PIAATIGGGF VVSGKVDRLL IGDTIRVVDF KTGRRVPDAP
PDYHIRQMAG YVAALSVVFP GRPVEASLLY TAGPVLVTLT PEQLAGHKPG FPATEQSSVV
GG
//
