ID   A0A430JPW5_9ACTO        Unreviewed;       796 AA.
AC   A0A430JPW5;
DT   08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT   08-MAY-2019, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:RTE48837.1};
GN   Name=glgX {ECO:0000313|EMBL:RTE48837.1};
GN   ORFNames=EKN07_08300 {ECO:0000313|EMBL:RTE48837.1};
OS   Actinobaculum sp. 352.
OC   Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC   Actinobaculum.
OX   NCBI_TaxID=2490946 {ECO:0000313|EMBL:RTE48837.1, ECO:0000313|Proteomes:UP000273303};
RN   [1] {ECO:0000313|EMBL:RTE48837.1, ECO:0000313|Proteomes:UP000273303}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=352 {ECO:0000313|EMBL:RTE48837.1,
RC   ECO:0000313|Proteomes:UP000273303};
RA   Zhu W.;
RL   Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RTE48837.1}.
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DR   EMBL; RQSN01000007; RTE48837.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A430JPW5; -.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000273303; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000273303}.
FT   DOMAIN          158..568
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          465..489
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          705..796
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        729..773
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   796 AA;  88318 MW;  6A771F70ED7C5591 CRC64;
     MQIWPGHSYP LGATFDGTGT NFAVFSSVAT KMDLCLLDDA DQQTIVELRE VDGGVWHCYL
     PGVRPGQRYG YRVHGPYDPA SGLRCDPSKL LLDPYAKAID GHIEDSQAVF SYNFNNPSER
     NEQDSLGHTM TSVVINPFFD WGHDRPPAHD YSDTVIYEAH VRGMTMRHPD IPEDIRGTYA
     GISHPAIIDY LKKLGATAIE LMPCHQFVND TGLQAKGLSN YWGYNTIGFF APHNGYASSD
     TRGGQVDEFK AMVKSFHEAE IEVIMDVVYN HTAEGNHMGP TLSFKGLDNA AYYRLVEGSQ
     EHYFDTTGTG NSLNMRSPHT LQLIMDSLRY WITNMHVDGF RFDLASTLAR ELHEVDKLSS
     FFDIIQQDPL ISQVKLIAEP WDVGEGGYNV GGFPPLWSEW NGKYRDTVRD FWRGEPSVLS
     ELAFRLTGSS DLYENSGRRP MASINFVTAH DGFTLNDLVS YNEKHNEANG EGGADGESNN
     RSWNCGAEGP TDDPDIVALR ERQRKNFLST LLLSQGVPMI AHGDEIGRTQ NGNNNTYCQD
     NEISWVDWKL TDSQFDLLDF TRHLIAFRKA HSVFRRRRFL SGVSTRGGRE KLGEVEWLRP
     DATQMQDSDW SVGYARSLMV YLNGSAIAEP DSRGAEIYDD DVLITFNGDS EPLSFTIPSS
     AYGKRWNNVF NTAARIDPAE EYLPGQTFQV EGRSMRVFIC QREDQGSLCP GKKPNESVGR
     LRSDAENEVE SAASATGTDS GNTPLNNDSP SAQPAPEPAE NATSSTSITE QETADGVDVL
     GTGEASSMEK KSSATG
//