ID A0A430JY77_9FLAO Unreviewed; 515 AA.
AC A0A430JY77;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Replicative DNA helicase {ECO:0000256|ARBA:ARBA00021957, ECO:0000256|RuleBase:RU362085};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU362085};
GN Name=dnaB {ECO:0000313|EMBL:RTE51685.1};
GN ORFNames=EHW67_20230 {ECO:0000313|EMBL:RTE51685.1};
OS Arenibacter aquaticus.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Arenibacter.
OX NCBI_TaxID=2489054 {ECO:0000313|EMBL:RTE51685.1, ECO:0000313|Proteomes:UP000267585};
RN [1] {ECO:0000313|EMBL:RTE51685.1, ECO:0000313|Proteomes:UP000267585}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GUO666 {ECO:0000313|EMBL:RTE51685.1,
RC ECO:0000313|Proteomes:UP000267585};
RA Guo J., Sun J.;
RT "Arenibacter aquaticus sp.nov., a marine bacterium isolated from surface
RT seawater in the South China Sea.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity.
CC {ECO:0000256|RuleBase:RU362085}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU362085};
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000256|ARBA:ARBA00008428, ECO:0000256|RuleBase:RU362085}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RTE51685.1}.
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DR EMBL; RQPJ01000024; RTE51685.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A430JY77; -.
DR OrthoDB; 9773982at2; -.
DR Proteomes; UP000267585; Unassembled WGS sequence.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-UniRule.
DR CDD; cd00984; DnaB_C; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00665; DnaB; 1.
DR PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51199; SF4_HELICASE; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362085};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU362085};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362085};
KW Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:RTE51685.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362085};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362085};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|RuleBase:RU362085}.
FT DOMAIN 194..469
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT REGION 483..515
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..508
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 515 AA; 57225 MW; D4E9DE714F098217 CRC64;
MEKTNPVIGH RIDKSTIINL EKGKIPPQAV DLEEVVLGAM MIDKKGVDEV IDILHPDVFY
KEAHKHIYEA VFKLFENSEP VDLLTVSSQL KKDGRLEGVG GDFYLIKLTQ KVASSAHIEF
HARIILQKFI QRSLIKISNE IIEEAYDEAT DVFDLLDTAE SKLYDVTQGN LKRSAETAQN
LVIQAKKKIE EISGKEGLSG IPSGFDKLDK LTSGWQPSDL IIVAARPGMG KTALTLSMAR
NIAVNSGYGV AFFSLEMSSV QLITRLISSE TGLSSEKLRT GKLEKHEWEQ LNVKVKALES
APLFIDDTPS LSIFDLRAKA RRLASQHGIK MIIIDYLQLM TAGGTQKGGN REQEISTISR
NLKALAKELN VPVIALSQLS RAVETRGGSK RPVLSDLRES GAIEQDADIV SFIYRPEYYK
IDEWDDEERS PTAGQGEFIV AKHRNGGLEN IRLKFVGHLG KFDNLDDFDS PFEFQSKMNA
DEDNPFVTKN LPNANEAFGS SMNNDGPQDD NDVPF
//
