ID   A0A430JYM9_9FLAO        Unreviewed;       540 AA.
AC   A0A430JYM9;
DT   08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT   08-MAY-2019, sequence version 1.
DT   24-JAN-2024, entry version 22.
DE   SubName: Full=M1 family peptidase {ECO:0000313|EMBL:RTE51796.1};
GN   ORFNames=EHW67_16425 {ECO:0000313|EMBL:RTE51796.1};
OS   Arenibacter aquaticus.
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Arenibacter.
OX   NCBI_TaxID=2489054 {ECO:0000313|EMBL:RTE51796.1, ECO:0000313|Proteomes:UP000267585};
RN   [1] {ECO:0000313|EMBL:RTE51796.1, ECO:0000313|Proteomes:UP000267585}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GUO666 {ECO:0000313|EMBL:RTE51796.1,
RC   ECO:0000313|Proteomes:UP000267585};
RA   Guo J., Sun J.;
RT   "Arenibacter aquaticus sp.nov., a marine bacterium isolated from surface
RT   seawater in the South China Sea.";
RL   Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRSR:PIRSR634015-3};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634015-3};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RTE51796.1}.
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DR   EMBL; RQPJ01000021; RTE51796.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A430JYM9; -.
DR   OrthoDB; 100605at2; -.
DR   Proteomes; UP000267585; Unassembled WGS sequence.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd09603; M1_APN_like; 1.
DR   Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR   Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR   InterPro; IPR045357; Aminopeptidase_N-like_N.
DR   InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR   InterPro; IPR034015; M1_LTA4H.
DR   InterPro; IPR014782; Peptidase_M1_dom.
DR   InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR   PANTHER; PTHR45726; LEUKOTRIENE A-4 HYDROLASE; 1.
DR   PANTHER; PTHR45726:SF3; LEUKOTRIENE A-4 HYDROLASE; 1.
DR   Pfam; PF01433; Peptidase_M1; 1.
DR   Pfam; PF17900; Peptidase_M1_N; 1.
DR   SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR   SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE   4: Predicted;
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR634015-3};
KW   Zinc {ECO:0000256|PIRSR:PIRSR634015-3}.
FT   DOMAIN          44..215
FT                   /note="Aminopeptidase N-like N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF17900"
FT   DOMAIN          266..465
FT                   /note="Peptidase M1 membrane alanine aminopeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF01433"
FT   ACT_SITE        331
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   ACT_SITE        405
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   BINDING         330
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         334
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         353
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
SQ   SEQUENCE   540 AA;  62777 MW;  BBD94E771C583FDE CRC64;
     MKKKIAVLLL TIGFSSYGQE QTFTEQDTLR GSITPERSWW DLNFYHLKVE VEPDKKYISG
     SNTIRYTVLS PHQRLQVDLQ PPLKIEKVTQ DGQSLTVDSN GNAHFINLKK KQQVGATNEV
     VVYYSGHPKE AVRAPWDGGF SWKKDDNGKH FVATSCQGLG ASIWWPNKDH MYDEVDSMAI
     SITTPKDLMD VSNGRLKKIE DHQDTRTYHW YVDNPINNYG VNINMGDYVH FGETYPGEKG
     KLSMDYYVLR DNKEKAEKQF KQAPLMMEAF EHWFGPYPFY EDGFKLVEVP YLGMEHQSSV
     TYGNKYMNGY LGRDLSDTGW GLKFDFIIIH ESGHEWFANN ITYKDIADMW IHESFTAYSE
     NLYLDYHYGK KASADYVIGT RSNIKNNTPI IGTYNVNKEG SGDMYSKGAN MLHTLRQLID
     DDDKWRSILR GLNSEFYHKT VTTQEIEDYI SEQSGIDLTQ FFDQYLRNAQ LPIFEYKIEG
     QQLHYRYSNI VSGFDMPLKI MVNGSEEWIY PNLDWKKNTY QKDVQSVVVD RNFYIEVAKK
//