ID A0A431V0K1_9GAMM Unreviewed; 555 AA.
AC A0A431V0K1;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 13.
DE RecName: Full=Small-conductance mechanosensitive channel {ECO:0000256|RuleBase:RU369025};
GN ORFNames=EKG36_14560 {ECO:0000313|EMBL:RTR01084.1};
OS Halomonas nitroreducens.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=447425 {ECO:0000313|EMBL:RTR01084.1, ECO:0000313|Proteomes:UP000267400};
RN [1] {ECO:0000313|EMBL:RTR01084.1, ECO:0000313|Proteomes:UP000267400}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=11S {ECO:0000313|EMBL:RTR01084.1,
RC ECO:0000313|Proteomes:UP000267400};
RA Yu L.;
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mechanosensitive channel that participates in the regulation
CC of osmotic pressure changes within the cell, opening in response to
CC stretch forces in the membrane lipid bilayer, without the need for
CC other proteins. Contributes to normal resistance to hypoosmotic shock.
CC Forms an ion channel of 1.0 nanosiemens conductance with a slight
CC preference for anions. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBUNIT: Homoheptamer. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU369025}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU369025}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|RuleBase:RU369025}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU369025}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RTR01084.1}.
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DR EMBL; RXNS01000014; RTR01084.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A431V0K1; -.
DR OrthoDB; 9792218at2; -.
DR Proteomes; UP000267400; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008381; F:mechanosensitive monoatomic ion channel activity; IEA:InterPro.
DR Gene3D; 1.10.287.1260; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR045275; MscS_archaea/bacteria_type.
DR InterPro; IPR006685; MscS_channel_2nd.
DR PANTHER; PTHR30221; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR PANTHER; PTHR30221:SF1; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|RuleBase:RU369025};
KW Cell membrane {ECO:0000256|RuleBase:RU369025};
KW Ion channel {ECO:0000256|RuleBase:RU369025};
KW Ion transport {ECO:0000256|RuleBase:RU369025};
KW Membrane {ECO:0000256|RuleBase:RU369025}; Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|RuleBase:RU369025};
KW Transmembrane helix {ECO:0000256|RuleBase:RU369025};
KW Transport {ECO:0000256|RuleBase:RU369025}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..555
FT /note="Small-conductance mechanosensitive channel"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5019196214"
FT TRANSMEM 202..229
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 241..262
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 274..296
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 328..347
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 359..385
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT DOMAIN 377..442
FT /note="Mechanosensitive ion channel MscS"
FT /evidence="ECO:0000259|Pfam:PF00924"
SQ SEQUENCE 555 AA; 62354 MW; 9B40BF415F341F57 CRC64;
MGQRFAWMLL MMILVFPAVC AAQEAEDHWF EVSALNPGLG AVPEEVERLT PRESIRSFFE
LAEKNDFAAA AHLLNLEDLS PQEQAAKGPE LARQLASVLQ HGEWVNISSL PARRDAMIDE
GANQHPRAGE ARRNLKLASL EANGQTYDIR LARYKAGDQQ PVWLFMPDTL SVVPALYEAF
GPAWVEPHIP ERFKQPLGML RLWEWLAIPL FIGMIGLLGF AVYSLTSLLA RWLPAGVSSI
FAGRVGMPLA FIVMSLVAQW TLGYVVSFSG AATTVFRILL ALIMAWGLGL IALRFVDTIL
LKVTSRLVGE IDDTRHQDER RLLTTLYALR RTIILITVVI AAVYVLGNLS IFDSLGMTLL
ASASVLTVLV GIAGQAVLGN ILASFQVALA KPVRIGDSVF FEGQWCYVEG IFYTFIRLRT
WDERRLIVPV TYFISKPFEN WSTKNAKMYR QFVLTLHLSA DVQCLRDKFQ ELAKDEEGVI
DHDRLLCYVT GQSADAQEIT FYLMTPEPMS GWLAETNLRE RMLAFVREQH PEWWPREVVV
ISHQDVARGH ASGHR
//
