ID A0A432ZJP6_9GAMM Unreviewed; 961 AA.
AC A0A432ZJP6;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Protease 3 {ECO:0000256|ARBA:ARBA00017565};
DE EC=3.4.24.55 {ECO:0000256|ARBA:ARBA00012449};
DE AltName: Full=Pitrilysin {ECO:0000256|ARBA:ARBA00033450};
DE AltName: Full=Protease III {ECO:0000256|ARBA:ARBA00031184};
DE AltName: Full=Protease pi {ECO:0000256|ARBA:ARBA00029597};
GN ORFNames=CWI83_04160 {ECO:0000313|EMBL:RUO78237.1};
OS Pseudidiomarina taiwanensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC Idiomarinaceae; Pseudidiomarina.
OX NCBI_TaxID=337250 {ECO:0000313|EMBL:RUO78237.1, ECO:0000313|Proteomes:UP000288279};
RN [1] {ECO:0000313|EMBL:RUO78237.1, ECO:0000313|Proteomes:UP000288279}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PIT1 {ECO:0000313|EMBL:RUO78237.1,
RC ECO:0000313|Proteomes:UP000288279};
RX PubMed=30364313; DOI=.3389/fmicb.2018.02453;
RA Liu Y., Lai Q., Shao Z.;
RT "Genome-Based Analysis Reveals the Taxonomy and Diversity of the Family
RT Idiomarinaceae.";
RL Front. Microbiol. 9:2453-2453(2018).
CC -!- FUNCTION: Endopeptidase that degrades small peptides of less than 7
CC kDa, such as glucagon and insulin. {ECO:0000256|ARBA:ARBA00002184}.
CC -!- SIMILARITY: Belongs to the peptidase M16 family.
CC {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RUO78237.1}.
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DR EMBL; PIQG01000002; RUO78237.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A432ZJP6; -.
DR OrthoDB; 9811314at2; -.
DR Proteomes; UP000288279; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR011765; Pept_M16_N.
DR InterPro; IPR001431; Pept_M16_Zn_BS.
DR InterPro; IPR007863; Peptidase_M16_C.
DR InterPro; IPR032632; Peptidase_M16_M.
DR PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR PANTHER; PTHR43690; NARDILYSIN; 1.
DR Pfam; PF00675; Peptidase_M16; 1.
DR Pfam; PF05193; Peptidase_M16_C; 2.
DR Pfam; PF16187; Peptidase_M16_M; 1.
DR SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR PROSITE; PS00143; INSULINASE; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000288279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 76..210
FT /note="Peptidase M16 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00675"
FT DOMAIN 235..414
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
FT DOMAIN 420..698
FT /note="Peptidase M16 middle/third"
FT /evidence="ECO:0000259|Pfam:PF16187"
FT DOMAIN 703..882
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
SQ SEQUENCE 961 AA; 108851 MW; A3E5138E20A58CAD CRC64;
MTSSYLTKVI SSVLKTSIFR WNSKPSVNQR SNTIRSVMVS PSHGGDSVVH ATDIIQSPAD
QCHYQALTLA NGLRCCLVHS PEAQHSAAAM AVATGHFDDP EDAQGLAHFL EHMLFLGTEG
YPAAEDYQNF IANHGGGHNA WTGTELSNYY FHILPEYFGQ ALDRFCRFFY QPLLHPDWVA
KELESIDAEF HLKRQDELRR LYQVHKSTVN QAHPFAKFSV GNRNTLSDRP DRPLAQQLRD
FFQSNYQAQW MTLVLVGPHS LAELQQLALQ YGEEIPGQPE RVKPAIDVPL YLPEQLAARI
QMRPLKDVQR LIISFALPSI DADYAHKTTS FIAHILGYEG PGSLFSLLRE RQWIVSLAAG
GGISGSNFKD FNINMQLTDE GLAHIDDIIA EVLAYIRLID AMAIEEWRYQ ERQNTIAQAF
RYQERSRSKD LAPALAVNLQ QYKLEDVVFG DYRMDGLNHS FARQILQLMT PDNMRTTVIH
KQVETDQIEP IYGSEYSFSP LSNDSLARFR AISASDSQAR LPQANPYTGQ LRPLHPVKRV
QSVPELTVIN SKLRHWHLQE AEFRVPRAHL YLSFRLPAVT ATARSFALAR LWCELLLDYL
SESCYDAEVA GLHYNIYPQQ QGITIHISGL SSGVATLSQQ LLITLQNATF SPHRFHVLRQ
KLAQNWRSAL ASRPLNLLFS KFNVLVQPNT FAVCQLAEEL AEIDLADYQQ WRSQLFNEVA
IESFSCGDII TEELHPVLEQ IQQLTAAAKL APLAQLDARG LAELQAQDFK VCEGLRQTHP
DSALILAIQG EQTELREQAL FLLFNHLVNS AFFHQLRTEK QLGYLVGTSY LPIQQRPHLL
FYVQSSNTDT EQLTTAVEHF LADFQLHLQQ FDADQLASAQ QAIRLQLLEP DTNLRLRAQR
FWSSITQADT DFARLNQLAE ALEHLTPKDL QQFFNTAVLQ TTSRLYLKSE PTALTAVAEP
D
//
