ID A0A433X8S9_9BACL Unreviewed; 2411 AA.
AC A0A433X8S9;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 14.
DE SubName: Full=Pullulanase {ECO:0000313|EMBL:RUT30521.1};
DE EC=3.2.1.41 {ECO:0000313|EMBL:RUT30521.1};
GN ORFNames=EJP77_11845 {ECO:0000313|EMBL:RUT30521.1};
OS Paenibacillus zeisoli.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=2496267 {ECO:0000313|EMBL:RUT30521.1, ECO:0000313|Proteomes:UP000272464};
RN [1] {ECO:0000313|EMBL:RUT30521.1, ECO:0000313|Proteomes:UP000272464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3-5-3 {ECO:0000313|EMBL:RUT30521.1,
RC ECO:0000313|Proteomes:UP000272464};
RA Sun L., Chen Z.;
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RUT30521.1}.
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DR EMBL; RZNX01000004; RUT30521.1; -; Genomic_DNA.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000272464; Unassembled WGS sequence.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd10315; CBM41_pullulanase; 2.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR CDD; cd12962; X25_BaPul_like; 1.
DR Gene3D; 2.60.40.1080; -; 1.
DR Gene3D; 2.60.40.1110; -; 2.
DR Gene3D; 2.60.40.1220; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 2.
DR Gene3D; 2.60.40.10; Immunoglobulins; 4.
DR InterPro; IPR003343; Big_2.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR005323; CBM41_pullulanase.
DR InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR008964; Invasin/intimin_cell_adhesion.
DR InterPro; IPR011838; Pullulan_Gpos.
DR InterPro; IPR001119; SLH_dom.
DR InterPro; IPR040806; SpuA_C.
DR NCBIfam; TIGR02102; pullulan_Gpos; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF03714; PUD; 2.
DR Pfam; PF00395; SLH; 3.
DR Pfam; PF18033; SpuA_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SMART; SM00635; BID_2; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 2.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR SUPFAM; SSF49373; Invasin/intimin cell-adhesion fragments; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 2.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR PROSITE; PS51272; SLH; 3.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000313|EMBL:RUT30521.1};
KW Hydrolase {ECO:0000313|EMBL:RUT30521.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000272464};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..33
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 34..2411
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5019544734"
FT DOMAIN 2230..2288
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT DOMAIN 2289..2352
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT DOMAIN 2356..2411
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT REGION 2024..2047
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2411 AA; 263379 MW; A907D08A6F508117 CRC64;
MKISLWGKRA FAVSMVLALV FSCFGMYPAG ANAATSKVTL VGDLQTKLGS DKDWDPASAV
TVMTSQGDGL YAFKGNLPAG TYQYKVALNG AWDESYGFSN YTNPQGIDQG GNIKLTLAEA
SEVTFYYNDT THKIADSTYY TPAAADKLPR VVGTVQTALG ETANSPADAK TLLTDPDFDN
VYTATAEVAQ GDYKYQVYVP GATEADNRSY PDAPADLNLP SKLPVTFTYN AKDHTSDAKF
TAPQDPGQVT PVPAGNVRIH YARTDKNYTG QSLWIYDDVK TPSSGDFPAG ATPFPAGQTD
SYGAYVDIPV IDGAKKVSFV VVNRSTNVKD GGNKTFFILT PETNEAWIKQ GDDKVYKSPS
GEEPIVLVSA GLAGTTKLAL NFSTTNGLAV DKLKTELKVK DKDGKDLAVN NATIASINTV
EVETDAYTLD QAPLTIEYSG KTRTTTSDWR FLDEEYNYTG DDLGATYHEA DKSATLKLWA
PKASKVAVDV YDKSDSAVLV KSLDLTQGEH GVWSIELTPS DLGGGITDVR GYFYQYEVTN
NGVTNKVLDP YAKSMAVFTV DTKGDPGADG DTVGKAAIVD LSQTNPDSYG YADIKGYEKR
EDAVIYEVHI RDFTSDPSIE DNFTDVRWGS YKAFEKKLDY LKSLGVTHIQ LLPVMAWYYG
DETKMGEREL NYSNKDNEYN WGYDPQNYFS PDGAYSEDPK DPELRIKELK GLIDAVHKAG
MGVVLDVVYT HMAKKEFLND IVPNYYHFID PSGKFIGGFS NNLATNHKMA EKLMVDSVKY
WFKEYKIDGM RWDMMGDATY EAVQHALDAA KEINPKALFI GEGWRTFGGQ DAEPDLLGKA
ADQDWVNKTD DMGVFSDEFR NEVKSGYNSE GQPRFITNGA RSISTIMNNI KGEPSNVKFD
DPGDIVPYIE AHDNLTLHDV IALSIKKDPS IPENELEIQK RIRLGNMLEL TSQGTAFIHA
GQEYGRTKQW KTDKEPEDKG RATVLNDQDG KALGYFIHDS YDSSDAVNMF DWSKATDETN
FPVQTTTQKY TSGLIELRKS TNAFRLGDKL LVDSNVTLIP AKEIKTDDLV IGYKNKSTDG
TGNYYVFMNG DSTSRTLTLS EDLTKGVVLV DSDEAGVTAI KDQSGFKLTA DSITLDPLTA
VIIKQDAAAA KLTSLVPDSR NYSLQVGGTH QTAVVAKYDD GSKATVTGKA TYVSDKPEVA
TVTDKGLVKA TGLGTATITI SYGGRSKTVT VFVNPKRYVE FTYIREDGNY KDWNLWVWNT
GVKNDQIDFT TIKDGKASVL IEVAPNATNV GFIVRKGADW STAKQDISYN REIPLTPGEQ
FTKVTVKSMV GELNIKPANT GPVMKDGSIT FIYRDDELFR QGTMTDIKAV KVKVNGTEYP
MVYDEAKEWF SYKLTKANEG NNNYTFLVTA KDGNTTEVTD PKNTVNGKSV LVYHNPTVKV
SASVNPAAVT SNQNAVLTVK ADSSLAGVSY REGTLDLSTL GGPSQVKLDT TLMKQTIAVK
DTVAAGIKTL PITLVDQYGN THKGSTTIEV KARTYTGDKL DFDWDEARIY FALTDRFKDG
DTTNDFNVDK THPEAYHGGD FRGMIDNLDY IQKLGINTLW ITPIVDNIDF NKGADFDAKQ
YGYHGYWAKD FTQLDEHLGD MATFKELINK AHDRGIKIMV DVVLNHTGYG VKEDDDDYPT
ITPEDKERFK GMLRMDNVSA DSDPVKGELA YLPDFKTEVP AVRDKVVAWQ SGWLNNARTD
RGDTIDYFRV DTVKHVDGTT WKAFKNALTA IDPKFKMVGE YFGGTVDNNG DMLQTGQMDG
LLDFGFKDAA QNFANGKIND TDAYMQDRES KLDNTKTMAQ FLSSHDENGF LTEYVDGDKG
KLKVAAALQI TAKGQPVIYY GEELGNSGKK EGDMSKGQFS QNRSDMPWDK LTTEQALHDH
YQKLLNIRAK YSKVFSKGTR TKLAGSDELG YLAFNKHYGN EDVVTVINTN TTATTATFTV
PFAANTNLKD EYSGNTYIVS ADGKVKVSLP GRDNGGTVIL AVAQASTPGN PGPTDPTVPT
TPTDDSQVVN ESSLKNGKDG KVEVQIAQGK KSVLLPAQAA KALGTNDLTI KLGSLDVTLP
NEVLNTIQGM VSAAEADGSQ IRFEANPLES SSANTTVGGL QKKGAKVAPA SDVYEFKLSI
IKKDGTSIPV TTFQEPITVS FKLKGNPNKD LVGVYYLADN GTLEYVGGVL TGDVLTAQVK
HFSKYAALEI DKSFADVPAS HWASAAIKSL TAKQIVTGVT DTKYQPNSKV SRAEFTALLA
RALGLQAGEH SAFADVKAGA WYASYVDAAV KAGIVTGRSK DAFAPNETIS REEMAVMLIR
ALEVKQGKKV DAAVSAGAFA DATKISSWAA AYVNAAASLE LVQGKENHQF DPQGFMTRAE
SAQVIYKLLA K
//
