ID A0A437GXL6_9SPHN Unreviewed; 819 AA.
AC A0A437GXL6;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 13.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN ORFNames=EKN06_09315 {ECO:0000313|EMBL:RVQ67109.1};
OS Croceicoccus ponticola.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Erythrobacteraceae; Croceicoccus.
OX NCBI_TaxID=2217664 {ECO:0000313|EMBL:RVQ67109.1, ECO:0000313|Proteomes:UP000283003};
RN [1] {ECO:0000313|EMBL:RVQ67109.1, ECO:0000313|Proteomes:UP000283003}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GM-16 {ECO:0000313|EMBL:RVQ67109.1,
RC ECO:0000313|Proteomes:UP000283003};
RA Yoon J.-H.;
RT "Croceicoccus ponticola sp. nov., a lipolytic bacterium isolated from
RT seawater.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RVQ67109.1}.
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DR EMBL; RXOL01000003; RVQ67109.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A437GXL6; -.
DR OrthoDB; 9803459at2; -.
DR Proteomes; UP000283003; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd18032; DEXHc_RE_I_III_res; 1.
DR CDD; cd18799; SF2_C_EcoAI-like; 1.
DR Gene3D; 3.90.1570.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013670; EcoEI_R_C_dom.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR PANTHER; PTHR47396:SF1; ATP-DEPENDENT HELICASE IRC3-RELATED; 1.
DR PANTHER; PTHR47396; TYPE I RESTRICTION ENZYME ECOKI R PROTEIN; 1.
DR Pfam; PF08463; EcoEI_R_C; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Helicase {ECO:0000313|EMBL:RVQ67109.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000283003};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT DOMAIN 180..362
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT REGION 582..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 587..607
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 819 AA; 92114 MW; 7721D383172A09FC CRC64;
MLDKQSLSER DICTKFITPA VKEAGWDEMT QIREEVSFTK GRIIVRGKMV SRGQGKRADY
VLYYRPNIPL ALIEAKDNRQ PIGAGIQQGL GYAETLDIPF VFASNGDGFV FHDRTASGEA
LETTLGLDAF PSPEELWQRY AAWKGLTPEA EGIVLEDYFD DGSGKTPRYY QVNAVNAAMV
AIAKGQRRVL LVMATGTGKT YTSFQIIWRL WKGGQKKRIL FLADRNVLVD QTMVNDFRPF
GPAMAKLSAN AKTIERDDGT KIDLTLAMDK KRRVDTAYEI YLGLYQSITG PEERQKLFKE
FSPGFFDLIV IDECHRGSAA EDSAWREILE HFSEATQIGL TATPKETKYA SNMHYFGEPV
YSYTLKQGIR DGFLAPYKVI KVHIDRDVEG YRPEKGQVDR EGNEVEDRIY NTKDFDRNLV
LDDRTKLVAK KVTEFLKASG DRYQKTIVFC VDQEHAARMR QALINENADL VAENHRYVMR
ITGNDPDGQA QLGNFIDPES RYPVLVTTSR LLSTGVDAQT CRLIVLDREV GSMTEFKQIV
GRGTRVHEDT HKFFFTLMDF RGATNHFADP DFDGEPVQIY EPSADDPVTP PDDETDIADE
DTDQDAITGD GETIIDDGSG TDISIDGGEQ KKGKVYVDGV PVVIVAERVE YLDESGKLVT
ESLRDFSKKA LRKHFASLDD FLKRWNSSDR KQAIVDELAD EGLSLEVIAD ELGKGLDPFD
VICHIAFDRK PLTRHERAEQ VKKRDIFGKY GEQARNVLDA LLAKYADEGV LNLDDANVLR
IPPLNKMGTP FQLLKAFGGK AGFEEAVHAL QHEIYQEVA
//
