ID A0A437GYT4_9SPHN Unreviewed; 463 AA.
AC A0A437GYT4;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Small-conductance mechanosensitive channel {ECO:0000256|RuleBase:RU369025};
GN ORFNames=EKN06_06300 {ECO:0000313|EMBL:RVQ67847.1};
OS Croceicoccus ponticola.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Erythrobacteraceae; Croceicoccus.
OX NCBI_TaxID=2217664 {ECO:0000313|EMBL:RVQ67847.1, ECO:0000313|Proteomes:UP000283003};
RN [1] {ECO:0000313|EMBL:RVQ67847.1, ECO:0000313|Proteomes:UP000283003}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GM-16 {ECO:0000313|EMBL:RVQ67847.1,
RC ECO:0000313|Proteomes:UP000283003};
RA Yoon J.-H.;
RT "Croceicoccus ponticola sp. nov., a lipolytic bacterium isolated from
RT seawater.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mechanosensitive channel that participates in the regulation
CC of osmotic pressure changes within the cell, opening in response to
CC stretch forces in the membrane lipid bilayer, without the need for
CC other proteins. Contributes to normal resistance to hypoosmotic shock.
CC Forms an ion channel of 1.0 nanosiemens conductance with a slight
CC preference for anions. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBUNIT: Homoheptamer. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU369025}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU369025}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|ARBA:ARBA00008017, ECO:0000256|RuleBase:RU369025}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU369025}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RVQ67847.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; RXOL01000002; RVQ67847.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A437GYT4; -.
DR OrthoDB; 9793781at2; -.
DR Proteomes; UP000283003; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008381; F:mechanosensitive monoatomic ion channel activity; IEA:InterPro.
DR Gene3D; 1.10.287.1260; -; 1.
DR Gene3D; 2.30.30.60; -; 1.
DR Gene3D; 3.30.1340.30; -; 1.
DR Gene3D; 3.30.70.100; -; 1.
DR InterPro; IPR007055; BON_dom.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR045275; MscS_archaea/bacteria_type.
DR InterPro; IPR023408; MscS_beta-dom_sf.
DR InterPro; IPR006685; MscS_channel_2nd.
DR InterPro; IPR011066; MscS_channel_C_sf.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR PANTHER; PTHR30221; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR PANTHER; PTHR30221:SF1; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR Pfam; PF04972; BON; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR SUPFAM; SSF82689; Mechanosensitive channel protein MscS (YggB), C-terminal domain; 1.
DR SUPFAM; SSF82861; Mechanosensitive channel protein MscS (YggB), transmembrane region; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS50914; BON; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|RuleBase:RU369025};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Ion channel {ECO:0000256|RuleBase:RU369025};
KW Ion transport {ECO:0000256|RuleBase:RU369025};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU369025};
KW Reference proteome {ECO:0000313|Proteomes:UP000283003};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU369025};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU369025}; Transport {ECO:0000256|RuleBase:RU369025}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..463
FT /note="Small-conductance mechanosensitive channel"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5019384986"
FT TRANSMEM 136..159
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 179..197
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 203..224
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT DOMAIN 50..116
FT /note="BON"
FT /evidence="ECO:0000259|PROSITE:PS50914"
FT REGION 393..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 405..420
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..453
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 463 AA; 49499 MW; 76E0026C651602AC CRC64;
MTALTLAMVT ALAAPYPAWA AIAAIPDVAE ETTTPTPPTA AIETERDADA DARIAERIGA
IFAAIDALGA VEVEANEGVV TLTGSVASAS DAKQAETIAS RVAGVVTVDN AIERDLAVDR
NLAPTISAII DEARKLWRML PLFAVALVIA AIVMVLFRWV SGLNSLWRRI TPNPFIAELL
QSAIRFVGVL AGLYLALKIL GATALLGALL GISGVIGIAI GFAVRDTIDN YISSIMLSLR
QPFRANDLVV IEGNEGRVIR LTSRATVLMT LDGNHLRIPN STVFKAVILN YTRNPQRRFE
FDLGVDADDN PLDAMAVGVA ALSALDFVLN DPEPGARIRE VGDSNIVLTF LGWIDQSQTD
YHKARSLAIE AAKAALETNG FALPEPIYRL RIDPRSAPLP GTPQEVRDRP DREVAQPEKP
SRKPRTAAAS DSTAPETHVS DLVDRERAAG GETDLLDASK PIE
//
