ID A0A443NYK3_9MAGN Unreviewed; 651 AA.
AC A0A443NYK3;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE RecName: Full=proline--tRNA ligase {ECO:0000256|ARBA:ARBA00012831};
DE EC=6.1.1.15 {ECO:0000256|ARBA:ARBA00012831};
DE AltName: Full=Prolyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00029731};
GN ORFNames=CKAN_01236700 {ECO:0000313|EMBL:RWR83607.1};
OS Cinnamomum micranthum f. kanehirae.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Magnoliidae; Laurales; Lauraceae; Cinnamomum.
OX NCBI_TaxID=337451 {ECO:0000313|EMBL:RWR83607.1, ECO:0000313|Proteomes:UP000283530};
RN [1] {ECO:0000313|EMBL:RWR83607.1, ECO:0000313|Proteomes:UP000283530}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chaw 1501 {ECO:0000313|Proteomes:UP000283530};
RC TISSUE=Young leaves {ECO:0000313|EMBL:RWR83607.1};
RX PubMed=30626928; DOI=10.1038/s41477-018-0337-0;
RA Chaw S.M., Liu Y.C., Wu Y.W., Wang H.Y., Lin C.I., Wu C.S., Ke H.M.,
RA Chang L.Y., Hsu C.Y., Yang H.T., Sudianto E., Hsu M.H., Wu K.P., Wang L.N.,
RA Leebens-Mack J.H., Tsai I.J.;
RT "Stout camphor tree genome fills gaps in understanding of flowering plant
RT genome evolution.";
RL Nat. Plants 5:63-73(2019).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-
CC tRNA(Pro); Xref=Rhea:RHEA:14305, Rhea:RHEA-COMP:9700, Rhea:RHEA-
CC COMP:9702, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78532, ChEBI:CHEBI:456215;
CC EC=6.1.1.15; Evidence={ECO:0000256|ARBA:ARBA00000857};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RWR83607.1}.
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DR EMBL; QPKB01000004; RWR83607.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A443NYK3; -.
DR STRING; 337451.A0A443NYK3; -.
DR Proteomes; UP000283530; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004827; F:proline-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006433; P:prolyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00778; ProRS_core_arch_euk; 1.
DR HAMAP; MF_01571; Pro_tRNA_synth_type3; 1.
DR InterPro; IPR002314; aa-tRNA-synt_IIb.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR007021; DUF659.
DR InterPro; IPR002316; Pro-tRNA-ligase_IIa.
DR InterPro; IPR004499; Pro-tRNA-ligase_IIa_arc-type.
DR InterPro; IPR033721; ProRS_core_arch_euk.
DR PANTHER; PTHR43382:SF3; PROLINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR PANTHER; PTHR43382; PROLYL-TRNA SYNTHETASE; 1.
DR Pfam; PF04937; DUF659; 1.
DR Pfam; PF00587; tRNA-synt_2b; 1.
DR PRINTS; PR01046; TRNASYNTHPRO.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:RWR83607.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000283530}.
FT DOMAIN 105..339
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
FT REGION 414..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..440
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 651 AA; 73658 MW; B6DA8CBD0D178C5D CRC64;
MGVAVLRLTS SFTTPLFLTS SSSSLRRLPF RSLRFLSPSP RLPRKVLSAS LASDFSTQSL
ASAEQQQKQK QPNKDGFITL RSQDFSAWYL DVIASAELAD YGPVRGTMVI RPYGYAIWES
IQDYLNAKFK ETGHSNMYFP QFMPYSFIEK EASHVEGFSP EFALVTIGGG KELEEKLVVR
PTSETIVNHM FTQWIHSYRD LPLMVNQWAN VTRWEMRTKP FVRTLEFLWQ EGHTAHATPE
EAENEQAAIP VIAGRKSRAE TFAGASRTYT IEAMMGDRKA LQAGTSHNLG QNFSRAFETQ
FIDENGQRQH VWQTSWAVST RFVGGIIMTH GDDAGLMLPP KVAPIQVKQH LPGGFRNVTA
CPKCPAEVRE EIREYMTKKK KEKEEMNMLP HFDELANAYE EDEEDDVEVI ERGKRPLAHG
SEVSSARGST TISTVSKTKK PKQKGPIDVF FTPNPDVVVQ NRKAQGKQTR IDANDPYRKE
LRERACIRFA RWMYDAEIPF NAVNCDSFGP MIEAIGQYGP GMKPPSYHEV RVPLLKKETE
HSNNLMKGHR DDWVKYGCSI MMDGWTDKKG RTLLNFLVNC PKGTMFVESI DASSYSKDAE
KMFQLIEKFV ERIGEANVVQ IVIDSAAANV LAGKFLEAKF AHLYWTPCAA H
//
