ID A0A445G3C4_GLYSO Unreviewed; 570 AA.
AC A0A445G3C4;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 12.
DE SubName: Full=Leucine aminopeptidase 2, chloroplastic {ECO:0000313|EMBL:RZB55720.1};
DE EC=3.4.11.1 {ECO:0000313|EMBL:RZB55720.1};
GN ORFNames=D0Y65_045151 {ECO:0000313|EMBL:RZB55720.1};
OS Glycine soja (Wild soybean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3848 {ECO:0000313|EMBL:RZB55720.1, ECO:0000313|Proteomes:UP000289340};
RN [1] {ECO:0000313|EMBL:RZB55720.1, ECO:0000313|Proteomes:UP000289340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. W05 {ECO:0000313|Proteomes:UP000289340};
RC TISSUE=Hypocotyl of etiolated seedlings {ECO:0000313|EMBL:RZB55720.1};
RA Xie M., Chung C.Y.L., Li M.-W., Wong F.-L., Chan T.-F., Lam H.-M.;
RT "A high-quality reference genome of wild soybean provides a powerful tool
RT to mine soybean genomes.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
CC Evidence={ECO:0000256|ARBA:ARBA00001585};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa
CC is preferably Leu, but may be other amino acids including Pro
CC although not Arg or Lys, and Yaa may be Pro. Amino acid amides and
CC methyl esters are also readily hydrolyzed, but rates on arylamides
CC are exceedingly low.; EC=3.4.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000135};
CC -!- SUBUNIT: Homohexamer (dimer of homotrimers).
CC {ECO:0000256|ARBA:ARBA00011867}.
CC -!- SIMILARITY: Belongs to the peptidase M17 family.
CC {ECO:0000256|ARBA:ARBA00009528}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZB55720.1}.
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DR EMBL; QZWG01000017; RZB55720.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A445G3C4; -.
DR OrthoDB; 2899215at2759; -.
DR Proteomes; UP000289340; Chromosome 17.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0070006; F:metalloaminopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00433; Peptidase_M17; 1.
DR Gene3D; 3.40.220.10; Leucine Aminopeptidase, subunit E, domain 1; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR HAMAP; MF_00181; Cytosol_peptidase_M17; 1.
DR InterPro; IPR011356; Leucine_aapep/pepB.
DR InterPro; IPR043472; Macro_dom-like.
DR InterPro; IPR000819; Peptidase_M17_C.
DR InterPro; IPR023042; Peptidase_M17_leu_NH2_pept.
DR InterPro; IPR008283; Peptidase_M17_N.
DR PANTHER; PTHR11963; LEUCINE AMINOPEPTIDASE-RELATED; 1.
DR PANTHER; PTHR11963:SF23; ZGC:152830; 1.
DR Pfam; PF00883; Peptidase_M17; 1.
DR Pfam; PF02789; Peptidase_M17_N; 1.
DR PRINTS; PR00481; LAMNOPPTDASE.
DR SUPFAM; SSF52949; Macro domain-like; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
DR PROSITE; PS00631; CYTOSOL_AP; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000256|ARBA:ARBA00022438,
KW ECO:0000313|EMBL:RZB55720.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:RZB55720.1};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000289340}.
FT DOMAIN 419..426
FT /note="Cytosol aminopeptidase"
FT /evidence="ECO:0000259|PROSITE:PS00631"
SQ SEQUENCE 570 AA; 59838 MW; 6947A2873D13F510 CRC64;
MAAIATIFVS FTTTSSSLFL TRLTSRLHFA AFPLRSPTRK LMSHTLARAT LGLTHPSNIE
TPKISFGAKD VDVTEWKGDI LAVGVTEKDL ARDANSKFEN VILSKIDSKL GGLLAEASSE
EDFSGKVGQS TVLRITGLGS KRVGLIGLGQ SPSTPSPFKG LGEAVVEAAK SSQASSVAVV
LASSEGLSAQ SKLSTAYAIA SGAVLGLFED NRYKSEAKKS TLRSIDIIGF GMGPELEKKL
KYAGDVSSGI IFGRELVNSP ANVLTPGVLA EEAAKIASTY SDVFTAKILN AEQCAELKMG
SYLGVAAASE NPPHFIHLCY KPLSGPVNVK LALVGKGLTF DSGGYNIKTG PGCSIELMKF
DMGGSAAVLG TAKALGQIKP LGVEVHFIVA ACENMISGTG MRPGDIVTAS NGKTIEVNNT
DAEGRLTLAD ALVYACNQGV EKIIDLATLT GACVVALGPS IAGVFTPSDD LAKEVFEASD
VSGEKLWRLP LEESYWETMK SGVADMLNTG GRQGGAIVAA LFLKQFVDEK VQWMHIDMAG
PVWNDKKRSA TGFGIATLVE WVLKNASKAN
//
