ID A0A445GP52_GLYSO Unreviewed; 470 AA.
AC A0A445GP52;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=D0Y65_039932 {ECO:0000313|EMBL:RZB62999.1};
OS Glycine soja (Wild soybean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3848 {ECO:0000313|EMBL:RZB62999.1, ECO:0000313|Proteomes:UP000289340};
RN [1] {ECO:0000313|EMBL:RZB62999.1, ECO:0000313|Proteomes:UP000289340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. W05 {ECO:0000313|Proteomes:UP000289340};
RC TISSUE=Hypocotyl of etiolated seedlings {ECO:0000313|EMBL:RZB62999.1};
RA Xie M., Chung C.Y.L., Li M.-W., Wong F.-L., Chan T.-F., Lam H.-M.;
RT "A high-quality reference genome of wild soybean provides a powerful tool
RT to mine soybean genomes.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZB62999.1}.
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DR EMBL; QZWG01000015; RZB62999.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A445GP52; -.
DR SMR; A0A445GP52; -.
DR OrthoDB; 930987at2759; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000289340; Chromosome 15.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF24; 3-KETOACYL-COA SYNTHASE 12; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417,
KW ECO:0000313|EMBL:RZB62999.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000289340};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417, ECO:0000313|EMBL:RZB62999.1}.
FT DOMAIN 25..312
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 337..418
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 168
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 247
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 339
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 343
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 372
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 376
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 470 AA; 53163 MW; C07EEA2B46EDC6BB CRC64;
MEFLSLLYGA MALYFCFLIW KLFDQRRDQE CYVLDYQLYK PSDERKLGTE RCGKIIGRNK
HLGLNEYKFL LKAIVNSGIG EETYAPRNVI EGREANPTLD DGVTEMEEFF HGSIEKLLER
SGISPSQIDV LVVNVSMFAV VPSLTSRIIN HYKMREDIKA YNLTGMGCSA SLISLDIIRN
IFKSQKNKCA LLVTSESLSP NWYNGKDRSM ILANCLFRTG GCVILLTNKR SLKQRAMFKL
KCLVRTHHGA KEDSYSCCNQ KEDEQGKLGF YLAKNLPKAA TRAFVENLRV LSPKVLPTRE
LLRFMIVSLI KKLSQTSSLK SSGGGSSKSP LNFKTGVEHF CLHTGGKAVI DGIGKSLDLC
EYDLEPARMT LHRFGNTSAS SLWYVLGYME AKKRLKKGDR VLMISFGAGF KCNSCLWEVM
KDLGDHTNVW SYCIDDYPPE SLANPFMETF GWINNVEDAT NFKLPDLFLK
//
