ID A0A445HUI4_GLYSO Unreviewed; 591 AA.
AC A0A445HUI4;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 12.
DE RecName: Full=carotenoid 9,10-dioxygenase {ECO:0000256|ARBA:ARBA00039084};
DE EC=1.14.99.n4 {ECO:0000256|ARBA:ARBA00039084};
GN ORFNames=D0Y65_035316 {ECO:0000313|EMBL:RZB77352.1};
OS Glycine soja (Wild soybean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3848 {ECO:0000313|EMBL:RZB77352.1, ECO:0000313|Proteomes:UP000289340};
RN [1] {ECO:0000313|EMBL:RZB77352.1, ECO:0000313|Proteomes:UP000289340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. W05 {ECO:0000313|Proteomes:UP000289340};
RC TISSUE=Hypocotyl of etiolated seedlings {ECO:0000313|EMBL:RZB77352.1};
RA Xie M., Chung C.Y.L., Li M.-W., Wong F.-L., Chan T.-F., Lam H.-M.;
RT "A high-quality reference genome of wild soybean provides a powerful tool
RT to mine soybean genomes.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-zeaxanthin + 2 O2 = 2 (3R)-hydroxy-beta-ionone +
CC 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial; Xref=Rhea:RHEA:26393,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:27547, ChEBI:CHEBI:53171,
CC ChEBI:CHEBI:53173; EC=1.14.99.n4;
CC Evidence={ECO:0000256|ARBA:ARBA00036367};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZB77352.1}.
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DR EMBL; QZWG01000012; RZB77352.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A445HUI4; -.
DR Proteomes; UP000289340; Chromosome 12.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR PANTHER; PTHR10543:SF89; CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964, ECO:0000313|EMBL:RZB77352.1};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000289340}.
FT BINDING 272
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 320
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 386
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 576
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 591 AA; 66545 MW; 4A213527A7CA42B2 CRC64;
MGDDGKKMSG EGGIVKVEPK PSNGFTSKVV DLLEKLVVKF LYDSSLTHHY LTGNFAPVPE
TPPTKDLPVK GYLPDCLNGE FVRVGPNPKF SPVAGYHCLS VLEKCVLKVE FDAYTMLSPT
VFAGLMEMVN FTTQNRSSGI CFSNFDLLNC RMIHGLRIKD GKATYVSRFV RTSRLKQEEY
FGGSKFMKIG DLKGLFGLLM VNIHMLRTKW KVLDVSYGTG TANTALVYHH GKLLALSEAD
KPYAIKVFED GDLQTLGMLD YDKRLGHSFT AHPKVDPFTG EMFTFGYAHT PPYITYRVIS
KDGYMHDPVP ITVSDPIMMH DFAITENYAI FLDLPLIFRP KEMVKNKTLI FSFDSTKKAR
FGVLPRYAKD EKLIRWFELP NCFIFHNANA WEEEDEVVLI TCRLQNPNLD LVGGTAKEKL
ENFSNELYEM RFNMKTGEAS QKKLSASAVD FPRVNESYTG RKQRYVYGTT LDSIAKVTGI
IKFDLHAEPD HGKTKLEVGG NVQGLYDLGP GKYGSEAVYV PRVPGTDSEE DDGYLIFFAH
DENTRKSFVH VINAKTMSAD PVAVVELPHR VPYGFHAFFV TEEQLQEQGK L
//
