ID A0A445HZD1_GLYSO Unreviewed; 389 AA.
AC A0A445HZD1;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=chalcone synthase {ECO:0000256|ARBA:ARBA00012975};
DE EC=2.3.1.74 {ECO:0000256|ARBA:ARBA00012975};
DE Flags: Fragment;
GN ORFNames=D0Y65_029483 {ECO:0000313|EMBL:RZB79143.1};
OS Glycine soja (Wild soybean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3848 {ECO:0000313|EMBL:RZB79143.1, ECO:0000313|Proteomes:UP000289340};
RN [1] {ECO:0000313|EMBL:RZB79143.1, ECO:0000313|Proteomes:UP000289340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. W05 {ECO:0000313|Proteomes:UP000289340};
RC TISSUE=Hypocotyl of etiolated seedlings {ECO:0000313|EMBL:RZB79143.1};
RA Xie M., Chung C.Y.L., Li M.-W., Wong F.-L., Chan T.-F., Lam H.-M.;
RT "A high-quality reference genome of wild soybean provides a powerful tool
RT to mine soybean genomes.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin. {ECO:0000256|ARBA:ARBA00002969}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004966}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|RuleBase:RU003633}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZB79143.1}.
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DR EMBL; QZWG01000011; RZB79143.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A445HZD1; -.
DR UniPathway; UPA00154; -.
DR Proteomes; UP000289340; Chromosome 11.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR PANTHER; PTHR11877:SF10; TYPE III POLYKETIDE SYNTHASE B; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003633};
KW Flavonoid biosynthesis {ECO:0000256|ARBA:ARBA00023241};
KW Reference proteome {ECO:0000313|Proteomes:UP000289340};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003633}.
FT DOMAIN 12..227
FT /note="Chalcone/stilbene synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00195"
FT DOMAIN 237..387
FT /note="Chalcone/stilbene synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02797"
FT ACT_SITE 163
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000451-1"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:RZB79143.1"
SQ SEQUENCE 389 AA; 42591 MW; 836FC02445204EDF CRC64;
RNMGNEGIMN GVTPGKATIL ALGKAFPHQL VMQEYLVDGY FRDTNCDSPE LKQKLTRLCK
TTTVKTRYVV MSEEILKKYP ELAAEGIPTV KQRLEICNEA VTEMAIEASQ ACINNWGGSL
SDITHLVYVS SSEARLPGGD LYLAKGLGLS PDTQRVMLYF AGCSGGVAGL RVAKDIAENN
PGSRVLIATS ETTIIGFKPP SADRPYDLVG VALFGDGAGA MIIGSDPILE SEKPLFELHT
AVQEFLPHTE KKIDGRLTEE GISFKLAREL PQIIEDNVEG FCDKLISVVG FENKEYNKMF
WAVHPGGPAI LNRIEKRLDL LPEKLSASRR ALMDYGNASS NTIVYVLEYM IEEGLKIRKD
ARGDLEWGLI LAFGPGITFE GILARNLCA
//
