ID A0A445K5R8_GLYSO Unreviewed; 535 AA.
AC A0A445K5R8;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 08-NOV-2023, entry version 16.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=D0Y65_013853 {ECO:0000313|EMBL:RZC06006.1};
OS Glycine soja (Wild soybean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3848 {ECO:0000313|EMBL:RZC06006.1, ECO:0000313|Proteomes:UP000289340};
RN [1] {ECO:0000313|EMBL:RZC06006.1, ECO:0000313|Proteomes:UP000289340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. W05 {ECO:0000313|Proteomes:UP000289340};
RC TISSUE=Hypocotyl of etiolated seedlings {ECO:0000313|EMBL:RZC06006.1};
RA Xie M., Chung C.Y.L., Li M.-W., Wong F.-L., Chan T.-F., Lam H.-M.;
RT "A high-quality reference genome of wild soybean provides a powerful tool
RT to mine soybean genomes.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZC06006.1}.
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DR EMBL; QZWG01000006; RZC06006.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A445K5R8; -.
DR SMR; A0A445K5R8; -.
DR OrthoDB; 930987at2759; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000289340; Chromosome 6.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF2; 3-KETOACYL-COA SYNTHASE 10; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417,
KW ECO:0000313|EMBL:RZC06006.1}; Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000289340};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417, ECO:0000313|EMBL:RZC06006.1};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 114..403
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 429..509
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 259
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 338
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 430
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 434
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 463
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 467
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 535 AA; 60714 MW; AB8DC35B51DE86D6 CRC64;
MANERDLFSA EIVNRGIESS GPNAGSLTFS VRVRRRLPDF LQSVNLKYVK LGYHYLINHG
IYLFTIPVLL VVFSAEVGSL SKEDLWKKLW EDARYDLATV LASFGVFVFT LSVYFMSRPR
PIYLIDFACY KPDDELKVSR EQLMEVARKS GKFDEASLEF QKRMLMSSGI GDETYIPKAV
VASTENTATM KEGRGEASMV MFGALDELFE KSRVRPKDVG VLVVNCSIFN PTPSLSAMII
NHYKMRGNIL SYNLGGMGCS AGIIGVDLAK DILQANPNNY AVVVSTEMVG YNWYQGKDRS
MLIPNSFFRM GCSAVLLSNR RRDYSRAKYR LEHIVRTHKG ADDRSFRCVY QEEDEQKLKG
LKISKDLIEI GGDALKTNIT TLGPLVLPFS EQLLFFATLV WRHLFGSKNG GNSPSMKKPY
IPDYKLAFEH FCVHAASKTI LDELQRNLEL SDKNIEASRM TLHRFGNTSS SSIWYELAYM
EAKESVRRGD RVWQLAFGSG FKCNSVVWRA MRRVTKPSRN PWLDCINRYP APLNN
//
