ID A0A445KPK1_GLYSO Unreviewed; 879 AA.
AC A0A445KPK1;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 08-NOV-2023, entry version 20.
DE RecName: Full=Phospholipase D {ECO:0000256|ARBA:ARBA00012027, ECO:0000256|PIRNR:PIRNR036470};
DE EC=3.1.4.4 {ECO:0000256|ARBA:ARBA00012027, ECO:0000256|PIRNR:PIRNR036470};
GN ORFNames=D0Y65_012535 {ECO:0000313|EMBL:RZC12826.1};
OS Glycine soja (Wild soybean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3848 {ECO:0000313|EMBL:RZC12826.1, ECO:0000313|Proteomes:UP000289340};
RN [1] {ECO:0000313|EMBL:RZC12826.1, ECO:0000313|Proteomes:UP000289340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. W05 {ECO:0000313|Proteomes:UP000289340};
RC TISSUE=Hypocotyl of etiolated seedlings {ECO:0000313|EMBL:RZC12826.1};
RA Xie M., Chung C.Y.L., Li M.-W., Wong F.-L., Chan T.-F., Lam H.-M.;
RT "A high-quality reference genome of wild soybean provides a powerful tool
RT to mine soybean genomes.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Hydrolyzes glycerol-phospholipids at the terminal
CC phosphodiesteric bond. {ECO:0000256|PIRNR:PIRNR036470}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:14445,
CC ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:58608; EC=3.1.4.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000798,
CC ECO:0000256|PIRNR:PIRNR036470};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913,
CC ECO:0000256|PIRNR:PIRNR036470};
CC -!- SIMILARITY: Belongs to the phospholipase D family. C2-PLD subfamily.
CC {ECO:0000256|ARBA:ARBA00010683, ECO:0000256|PIRNR:PIRNR036470}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZC12826.1}.
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DR EMBL; QZWG01000005; RZC12826.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A445KPK1; -.
DR Proteomes; UP000289340; Chromosome 5.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0070290; F:N-acylphosphatidylethanolamine-specific phospholipase D activity; IEA:UniProtKB-EC.
DR GO; GO:0004630; F:phospholipase D activity; IEA:UniProtKB-EC.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046470; P:phosphatidylcholine metabolic process; IEA:InterPro.
DR CDD; cd04015; C2_plant_PLD; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.30.870.10; Endonuclease Chain A; 3.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR001736; PLipase_D/transphosphatidylase.
DR InterPro; IPR024632; PLipase_D_C.
DR InterPro; IPR015679; PLipase_D_fam.
DR InterPro; IPR011402; PLipase_D_pln.
DR PANTHER; PTHR18896; PHOSPHOLIPASE D; 1.
DR PANTHER; PTHR18896:SF153; PHOSPHOLIPASE D; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF12357; PLD_C; 1.
DR Pfam; PF00614; PLDc; 1.
DR PIRSF; PIRSF036470; PLD_plant; 3.
DR SMART; SM00239; C2; 1.
DR SMART; SM00155; PLDc; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56024; Phospholipase D/nuclease; 2.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50035; PLD; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRNR:PIRNR036470};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR036470};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963,
KW ECO:0000256|PIRNR:PIRNR036470};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022963,
KW ECO:0000256|PIRNR:PIRNR036470};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000289340}.
FT DOMAIN 6..156
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 725..752
FT /note="PLD phosphodiesterase"
FT /evidence="ECO:0000259|PROSITE:PS50035"
SQ SEQUENCE 879 AA; 98707 MW; 6D965987DFE13752 CRC64;
MDCHGGISQS SPNREYEGLN GPVFLHGDLD LLIIEAKSLP NLDLSSETIR KCITMGNMCH
PPFIKGLKTH SGKDKMITSD PYVSVCIAGA TIAQTRVIAN CENPLWDEQF IVPVAHPAQK
LEFLVKDNDI LGAELIGVVE IPVQKIIAGN TVNDWFPIIG QYGNCLKPYP ELHISVQYRQ
IGVNRSESIS SGDGKALGVP KTYFPLRKGG SVTLYQDAHL PDGMLPEITL EGGKVFQHNK
CWEDICHAIL EAHHLIYIIG WSVYHPVRLV REATKPLPSG GELSLGELLK YKSQEGLRVV
MLIWDDRTSH DKFLLKTDGV MQTHDEETKK FFKHSTVHCV LSPRYASNKL SIFKQQLHSN
SCAPRQPWHD LHCKIEGPAA YDILTNFEQR WRKAKKWRDF RLKKVTNWHD DALLRLDRIS
WIVKPSPSSN GDKSVHVTDE NDPESWNVQI FRSIDSGSVK GFPKDVDKAK AQNLFCGKNL
KVDQSIHTAY VRAIRSAEHF VYIENQYFLG SSYHWPSYKN NAGANHLVPM ELALKIAGKI
GANERFCVYI VIPMWPEGVP TSAAVQEILF WQGQTMSMMY KIVADALEKA GLSYQYHPQD
YLNFYCLGKR EPQSTNISPT PNPSENRALI WEKECSDLNK KNLDPVRLVA LLLHPAAEDV
LQLFTVALVV GDEQAAENGS NDGTIVVEHG FASGLGILVG DEGEVVVAGA GGRRRCVSVK
KFRRFMIYVH AKGMVVDDEY VIIGSANINQ RSLDGSRDTE IAMGAYQPKY TWTEKNAHPR
GQVYGYRMSL WAEHLGSLDH CFAEPHNLEC VRHVNKIAKR NWDIYVSEEE NRMRGHLMQY
PVKISRDGKV SALDDYESFP DVGGKILGSP NSLPDALTT
//
