ID A0A448V0G1_9FIRM Unreviewed; 407 AA.
AC A0A448V0G1;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000256|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000256|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000256|HAMAP-Rule:MF_01074};
GN ORFNames=NCTC13079_00331 {ECO:0000313|EMBL:VEJ34801.1};
OS Peptoniphilus ivorii.
OC Bacteria; Bacillota; Tissierellia; Tissierellales; Peptoniphilaceae;
OC Peptoniphilus.
OX NCBI_TaxID=54006 {ECO:0000313|EMBL:VEJ34801.1, ECO:0000313|Proteomes:UP000269544};
RN [1] {ECO:0000313|EMBL:VEJ34801.1, ECO:0000313|Proteomes:UP000269544}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC13079 {ECO:0000313|EMBL:VEJ34801.1,
RC ECO:0000313|Proteomes:UP000269544};
RG Pathogen Informatics;
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000256|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000256|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; LR134523; VEJ34801.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A448V0G1; -.
DR KEGG; piv:NCTC13079_00331; -.
DR OrthoDB; 9765625at2; -.
DR Proteomes; UP000269544; Chromosome 1.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.1380; Transcriptional regulatory protein pf0864 domain like; 1.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR NCBIfam; TIGR00299; nickel pincer cofactor biosynthesis protein LarC; 1.
DR PANTHER; PTHR36566; NICKEL INSERTION PROTEIN-RELATED; 1.
DR PANTHER; PTHR36566:SF1; NICKEL INSERTION PROTEIN-RELATED; 1.
DR Pfam; PF01969; Ni_insertion; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|HAMAP-Rule:MF_01074};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|HAMAP-Rule:MF_01074};
KW Reference proteome {ECO:0000313|Proteomes:UP000269544}.
FT REGION 71..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 407 AA; 44765 MW; 62B14393D19C7E70 CRC64;
MRALYIDPFS GISGNMYIGA LLDMGAEIGP FLEGIAPLGV TVEDILLKKV DRKGIRATYF
NLKGEEEKLR AHEHEHAHHH EHEHGHTHNH THGGHRTLAD VMDCIDKSAF DDEVKILAKA
IYMPLARAEA AIHGKCLADV HFHEVGETDA IVDVLGAAYF TFAWDVEEIY VGKINAGFGT
VHCAHGNYPL PAPATAKILA DLNAPLGGMP VETELTTPTG AAILKGIGAQ YRPMPEGRIL
KSAYGAGTKD GAWANVLRVL LLETEEAEAP IWYSCNIDDM TGEELGFCME KLFEAGAKDV
TFTPIYMKKN RPGQRIDVLG ARADAEALKE ILFTHTTTLG IKEIPLEKIE LARTVESGTI
GTWAVRTKTA RYGDRKKASP EYEDLRALAT EEDISLRAAY GLWERRR
//
