ID A0A452CIB5_BALAS Unreviewed; 464 AA.
AC A0A452CIB5;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 08-NOV-2023, entry version 17.
DE RecName: Full=Bleomycin hydrolase {ECO:0000256|ARBA:ARBA00022227, ECO:0000256|PIRNR:PIRNR005700};
DE EC=3.4.22.40 {ECO:0000256|ARBA:ARBA00012465, ECO:0000256|PIRNR:PIRNR005700};
GN Name=BLMH {ECO:0000313|RefSeq:XP_028022754.1};
OS Balaenoptera acutorostrata scammoni (North Pacific minke whale)
OS (Balaenoptera davidsoni).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha; Cetacea; Mysticeti;
OC Balaenopteridae; Balaenoptera.
OX NCBI_TaxID=310752 {ECO:0000313|Proteomes:UP000261681, ECO:0000313|RefSeq:XP_028022754.1};
RN [1] {ECO:0000313|RefSeq:XP_028022754.1}
RP IDENTIFICATION.
RC TISSUE=Muscle {ECO:0000313|RefSeq:XP_028022754.1};
RG RefSeq;
RL Submitted (JAN-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by
CC hydrolysis of a carboxyamide bond of beta-aminoalanine, but also
CC shows general aminopeptidase activity. The specificity varies
CC somewhat with source, but amino acid arylamides of Met, Leu and Ala
CC are preferred.; EC=3.4.22.40;
CC Evidence={ECO:0000256|ARBA:ARBA00000423,
CC ECO:0000256|PIRNR:PIRNR005700};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR005700}.
CC -!- SIMILARITY: Belongs to the peptidase C1 family.
CC {ECO:0000256|PIRNR:PIRNR005700}.
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DR RefSeq; XP_028022754.1; XM_028166953.1.
DR AlphaFoldDB; A0A452CIB5; -.
DR Proteomes; UP000261681; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070005; F:cysteine-type aminopeptidase activity; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00585; Peptidase_C1B; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR004134; Peptidase_C1B.
DR PANTHER; PTHR10363; BLEOMYCIN HYDROLASE; 1.
DR PANTHER; PTHR10363:SF2; BLEOMYCIN HYDROLASE; 1.
DR Pfam; PF03051; Peptidase_C1_2; 1.
DR PIRSF; PIRSF005700; PepC; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR005700};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005700};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PIRNR:PIRNR005700};
KW Reference proteome {ECO:0000313|Proteomes:UP000261681};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807,
KW ECO:0000256|PIRNR:PIRNR005700}.
FT ACT_SITE 82
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
FT ACT_SITE 381
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
FT ACT_SITE 405
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
SQ SEQUENCE 464 AA; 53177 MW; F7F3FB39CC3E4296 CRC64;
MNDAGEAPLL PAKGLNSEKV AALLQKLNSD PQFVLAQNVG TTHDLLDICL KRATVQGVQH
VFQHTVHPEG KPVTSQKSSG RCWIFSCLNV MRLPFMRKLN IEEFEFSQSY LFFWDKVERC
YFFLNAFVDT AQKKEPEDGR LVQYLLTNPA NDGGQWDMLV NIVEKYGVIP KKCFPESYTT
EATKRMNDIL NHKMREFCLR LRNLVHSGAT KGEISATQDA MMEEIFRVVC ICLGNPPETF
TWEYRDKDKN YQKIGPITPL KFYKEHVKPL FNMEDKICLV NDPRPHHKYS RLYTVDYLSN
MVGGRKTLYN NQPIDLLKKV VAASIKDGEA VWFGCDVGKH FNGKLGLSDM NVYDHELVFG
VSMKNMNKAE RLTFGESLMT HAMTFTAVSE KDDQEGAFVK WRVENSWGED HGHKGYLCMT
DEWFSEYVYE VVVDRKHIPE GVLAVLEQEP IVLPAWDPMG ALAE
//
