ID A0A452F8Z9_CAPHI Unreviewed; 1631 AA.
AC A0A452F8Z9;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=ubiquitinyl hydrolase 1 {ECO:0000256|ARBA:ARBA00012759};
DE EC=3.4.19.12 {ECO:0000256|ARBA:ARBA00012759};
GN Name=USP32 {ECO:0000313|Ensembl:ENSCHIP00000020761.1};
OS Capra hircus (Goat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Capra.
OX NCBI_TaxID=9925 {ECO:0000313|Ensembl:ENSCHIP00000020761.1, ECO:0000313|Proteomes:UP000291000};
RN [1] {ECO:0000313|Ensembl:ENSCHIP00000020761.1, ECO:0000313|Proteomes:UP000291000}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Bickhart D.M., Koren S., Rosen B., Hastie A., Liachko I., Sullivan S.T.,
RA Burton J., Sayre B.L., Huson H.J., Lee J., Lam E., Kelley C.M.,
RA Hutchison J.L., Zhou Y., Sun J., Crisa A., Schwartz J.C., Hammond J.A.,
RA Schroeder S.G., Liu G.E., Dunham M., Shendure J., Sonstegard T.S.,
RA Phillippy A.M., Van Tassell C.P., Smith T.P.;
RT "Polished mammalian reference genomes with single-molecule sequencing and
RT chromosome conformation capture applied to the Capra hircus genome.";
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSCHIP00000020761.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707};
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DR EMBL; LWLT01000022; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 9925.ENSCHIP00000020761; -.
DR Ensembl; ENSCHIT00000028597.1; ENSCHIP00000020761.1; ENSCHIG00000019250.1.
DR GeneTree; ENSGT00940000155797; -.
DR OMA; HICLGLR; -.
DR Proteomes; UP000291000; Chromosome 19.
DR Bgee; ENSCHIG00000019250; Expressed in prefrontal cortex and 17 other cell types or tissues.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR CDD; cd00051; EFh; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 2.
DR Gene3D; 3.30.2230.10; DUSP-like; 1.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR InterPro; IPR035927; DUSP-like_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR006615; Pept_C19_DUSP.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR028135; Ub_USP-typ.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR PANTHER; PTHR21646; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR PANTHER; PTHR21646:SF76; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 32; 1.
DR Pfam; PF06337; DUSP; 1.
DR Pfam; PF13202; EF-hand_5; 2.
DR Pfam; PF14836; Ubiquitin_3; 1.
DR Pfam; PF00443; UCH; 1.
DR PRINTS; PR00450; RECOVERIN.
DR SMART; SM00695; DUSP; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR SUPFAM; SSF143791; DUSP-like; 1.
DR SUPFAM; SSF47473; EF-hand; 2.
DR PROSITE; PS51283; DUSP; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022807};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000291000};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807}.
FT DOMAIN 228..263
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 264..299
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 369..586
FT /note="DUSP"
FT /evidence="ECO:0000259|PROSITE:PS51283"
FT DOMAIN 761..1594
FT /note="USP"
FT /evidence="ECO:0000259|PROSITE:PS50235"
FT REGION 1392..1458
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1392..1430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1442..1456
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1631 AA; 184648 MW; 0A43DC9FEFCCFE9C CRC64;
MGAKESRIGF LSYEEALRRV TDVELKRLKD AFKRTCGLSY YMGQHCFIRE VLGDGVPPKV
AEVIYCSFGG TSKGLHFNNL IVGLVLLTRG RDEEKAKYIF SLFASESGSY VIREEMERML
HVVDGKVPDT LRKCFSEGEK VNYEKFRNWL LLNKDAFTFS RWLLSGGVYV TLTDDSDTPT
FYQTLAGVTH LEESDIIDLE KRYWLLKAQS RTGRFDLETF GPLVSPPIRP SLSEGLFNAF
DENRDNHIDF KEISCGLSAC CRGPLAERQK FCFKVFDVDR DGVLSRAELR NMVVALLEVW
KDNRTDDIPE LHMDLCDIVE GILNAHDTTK MGHLTLEDYQ IWSVKNVLAN EFLNLLFQVC
HIVLGLRPAT PEEEGQIIRG WLERESRYGL QPGHNWFIIS MQWWQQWKDY VKYDASPVVI
EPSSVLNGGK YSFGTTVHPV EQSEDRIGGG SPSYVNTTEE KFSDNVSSAS EASETAGSGF
LYSATPGTDM CFARQHNTSD NNNQCLLGAN GNILLHLNPQ KPGAIDNQPL VTQEPVKATS
LTLEGGRLKR TPQLVHGRDY EMVPEPVWRA LYHWYGANLA LPRPVIKNSK TEIPELELFP
RYLLFLRQQP ATRTQQSNIW VNMGMMSLRV FPQHLPRDNM RGKMCCCAFR NVPSPNAPLK
RVLAYTGCFS RMQTIKEIHE YLSQRLRIKE EDMRLWLYNS ENYLTLLDDE DHRLEYLKIQ
DEQHLVIEVR NKDMSWPEEM SFIANSSKID RHKVPTEKGA TGLSNLGNTC FMNSSIQCVS
NTQPLTQYFI SGRHLYELNR TNPIGMKGHM AKCYGDLVQE LWSGTQKNVA PLKLRWTIAK
YAPRFNGFQQ QDSQELLAFL LDGLHEDLNR VHEKPYVELK DSDGRPDWEV AAEAWDNHLR
RNRSIVVDLF HGQLRSQVKC KTCGHISVRF DPFNFLSLPL PMDSYMHLEI TVIKLDGTTP
VRYGLRLNMD EKYTGLKKQL SDLCGLKSEQ ILLAEVHGSN IKNFPQDNQK VRLSVSGFLC
AFEIPIPGSP VSASSPIQTD FSSSPSTNGM FTLTTNGDLP RPIFIPNGMP NTVVPCGTEK
NFTNGMVNGH MPSLPDSSFT GYIIAVHRKM MRTELYFLSS QKNRPSLFGM PLIVPCTVHT
RKKDLYDAVW IQVSRLASPL PPQEASNHAQ DCDDSMGYQY PFTLRVVQKD GNSCAWCPWY
RFCRGCKIDC GEDRAFIGNA YIAVDWDPTA LHLRYQTSQE RVVDEHESVE QSRRAQAEPI
NLDSCLRAFT SEEELGENEM YYCSKCKTHC LATKKLDLWR LPPILIIHLK RFQFVNGRWI
KSQKIVKFPR ESFDPSAFLV PRDPALCHRK PLTPQGDDLS EPRIPAGEVK KADAQNSAGE
DAVLLSKSPS SLSANIVSSP KGSPSTSRKS GTSCPSSKNS SPNSSPRTLG RSKGRLRLPQ
IGSKNKLSSS KENLDASKEN GAGHICELSD ALSRGHMLGG SQPELVTPQD HEVALANGFL
YEHEACGNGY SNGQLENHSE EDITDDQRED IHVKPIYNLY AISCHSGILG GGHYVTYAKN
PNSKWYCYND SSCKELHPDE IDTDSAYILF YEQQGIDYAQ FLPKIDGKKM ADTSSMDEDF
ESDYKKYCVL Q
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