ID A0A452FK84_CAPHI Unreviewed; 2487 AA.
AC A0A452FK84;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN Name=LRRK2 {ECO:0000313|Ensembl:ENSCHIP00000024800.1};
OS Capra hircus (Goat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Capra.
OX NCBI_TaxID=9925 {ECO:0000313|Ensembl:ENSCHIP00000024800.1, ECO:0000313|Proteomes:UP000291000};
RN [1] {ECO:0000313|Ensembl:ENSCHIP00000024800.1, ECO:0000313|Proteomes:UP000291000}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Bickhart D.M., Koren S., Rosen B., Hastie A., Liachko I., Sullivan S.T.,
RA Burton J., Sayre B.L., Huson H.J., Lee J., Lam E., Kelley C.M.,
RA Hutchison J.L., Zhou Y., Sun J., Crisa A., Schwartz J.C., Hammond J.A.,
RA Schroeder S.G., Liu G.E., Dunham M., Shendure J., Sonstegard T.S.,
RA Phillippy A.M., Van Tassell C.P., Smith T.P.;
RT "Polished mammalian reference genomes with single-molecule sequencing and
RT chromosome conformation capture applied to the Capra hircus genome.";
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSCHIP00000024800.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
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DR EMBL; LWLT01000005; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_017903409.1; XM_018047920.1.
DR Ensembl; ENSCHIT00000032662.1; ENSCHIP00000024800.1; ENSCHIG00000021778.1.
DR GeneID; 102188019; -.
DR CTD; 120892; -.
DR GeneTree; ENSGT00940000158267; -.
DR OMA; FIVECMV; -.
DR OrthoDB; 148126at2759; -.
DR Proteomes; UP000291000; Chromosome 5.
DR Bgee; ENSCHIG00000021778; Expressed in spleen and 16 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:UniProt.
DR GO; GO:0043226; C:organelle; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:UniProt.
DR CDD; cd14068; STKc_LRRK2; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 2.
DR Gene3D; 3.30.70.1390; ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032171; COR.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR020859; ROC_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR48005; LEUCINE RICH REPEAT KINASE 2; 1.
DR PANTHER; PTHR48005:SF13; SERINE_THREONINE-PROTEIN KINASE DDB_G0278509-RELATED; 1.
DR Pfam; PF16095; COR; 1.
DR Pfam; PF00560; LRR_1; 1.
DR Pfam; PF13855; LRR_8; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF08477; Roc; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00364; LRR_BAC; 8.
DR SMART; SM00369; LRR_TYP; 7.
DR SMART; SM00175; RAB; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF52058; L domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS51450; LRR; 5.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS51419; RAB; 1.
DR PROSITE; PS51424; ROC; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Coiled coil {ECO:0000256|SAM:Coils};
KW Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Leucine-rich repeat {ECO:0000256|ARBA:ARBA00022614};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000291000};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022777}.
FT DOMAIN 1288..1471
FT /note="Roc"
FT /evidence="ECO:0000259|PROSITE:PS51424"
FT DOMAIN 1839..2098
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT COILED 280..307
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 1866
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 2487 AA; 281790 MW; 7D3AABFC9C422412 CRC64;
MASGSCQGCE EEDEESLKKL IVRLNNVQEG KQIETLVQIL EDMLVFTYAD HASKLFQDKN
VHVPLLIVLD SYMRVASVQQ VGWSLLCKLI EICPNTMQSL MGPHDIGHDW EVLGVHQLIL
KMLTVHNASV NLTTVGLKAL DLLLNSGKIT LLILDEENDI FLLIFDAMRT FAASDEVQKL
GCKVLHVLFE RVSEEQLTEF VENKDYMILL SALKNFEDEE EIVLHVLHCL HSLAIPCSNV
EVLMSGNVRC YNIVVEAMKA FPISEKIQEV SCCLLHRLTL AETIFLNQDL EEKNENQEND
DDEEDKLFWL EACYKALTWH RKNKHVQEAA CWALNNLLMY QNSLHEKIGD EDGQFPAHRE
VMLSMLMHSS SKEVFQASAN ALSTLLEQNV NFRKILLSKG IYLNVLELMQ KHMHSPEVAE
SGCKMLNHLF EESSTSLDTM AAVVPKIITV MKSHETSLSV QLEALRAMLH FIVPGIPEEY
REDTEYQHKL NMIEKQCFRN DIHKLVLGAL NRFIGNPGIQ KCGLKVISSI IHFPDALEVL
SLEGAIDSVL HTLQMYPDDQ EIQCLGLSLI GCLITKKNLC MGTGHLLAKI LISTLQRFKD
IGEVQIKGFQ TVLTILELSV SFSKLLVDYS FDSVIFHQLS SSIMEQKDQQ FLNLCCKCFA
KLAVDNELKS VMLEKACDQN NSIMVECLLL LGADANRAKE ATSLICQVCE KASSPKLVEL
LLNSGSREQD VRKALTISIG KGNSQIISLL LRRLALDLAN SSICLGGFCM GKIDPSWLGP
LFPDKTSYLR KETNIGSTLA RMVLRYQMKS SVEEGAGSGS NGNFSEEIVD KFDEWTFIPE
SYVDSVFGQS DDLDSEGSEG SFLVKKKSNS ISVGEFYRDP ALQRCSPNLQ RHSSSLGPIF
DHEDLIKRKR KIFSSDDSLR ASKFQSHMKH SESMSSLPSE REYITSLDLS ANELRDVDAL
SQNSCISGHL EHLEKLELHQ NALTSFPQQL CETLKCLTHL DLHSNKFTSF PTYLLKMNCI
ASLDVSRNDI GPSVVLDPAV KCPTLKQFNL SYNQISSLPD NIGDVVEKLE QLILEGNKIS
EICSPLSLKE LKILNLSKNH ISSLSEDFLE TCPKLEILSA KMNFLAAMPF LPSSITSLKL
SQNRFTCVPE AILHLPHLRS LDMSSNDIRY LPGPVHWRSL NLRELLFSHN QISILDLSEK
ACAWSRIEKL HLSHNKLKEI PPEIGCLENL TSLDVSYNLD LRSFPNEMGK LSKIWDLPLD
ELHLNFDFKH VGCKAKDIIR FLQQRLKKAV PYNRMKLMIV GNTGSGKTTL LQQLMKIKKS
DLGMQGATVG IDVKDWPIQI RGKGKKDLIL NVWDFAGREE FYSTHPHFMT QRALYLAVYD
LSKGQAEVDA MKPWLFNIKA RASSSPVILV GTHLDVTDEK QRKACISKIT KELLNKRGFP
AIRDYHFVNA TEESDALAKL RKTIINESLN FKIRDQPVVG QLIPDCYVEL ERIILSERKN
VPIEFPVIDR KRLLQLVREH QLQLDENELP HAVHFLNESG VLLHFQDPAL QLSDLYFVEP
KWLCKVMAQI LTVKVEGFPK HPKGIISRRD VEKFLSKKKR FPKNYMAQYF KLLEKFQIAL
PIGEEYLLVP SSLSDHRPVI ELPHCENSEI IIRLYEMPYF PMGFWSRLIN RLLEISPYML
SGRERALRPN RMYWRQGIYL NWSPEAYCLV GSEVLDHHPE SFLKITVPSC RKGCILLGQV
VDHIDSLMEE WFPGLLEIDI CGEGETLLKK WALYSFNDGE EHQKILLDNL MKKAEEGDLL
VNPDQPRLTI PISQIAPDLI LADLPRNIML NNDELEFEQA PEFLLGDGSF GSVYRAAYQG
EEVAVKIFNK HTSLRLLRQE LVVLCHLHHP SLISLLAAGI RPRMLVMELA SKGSLDRLLQ
QDKTSLTRTL QHRIVLHVAD GLRYLHSAMI IYRDLKPHNV LLFTLYPNAA IIAKIADYGI
AQYCCRMGIK TSEGTPGFRA PEVARGNVIY NQQADVYSFG LLLYDILTTG GRIAEGLKFP
NEFDELAIQG KLPDPVKEYG CAPWPMVEKL IKRCLKENPQ ERPTSAQVFD ILNSAELICL
MRRISIPKSF TVECMVATNH NSKNVKIWLG CGHTDKGQLS FLDLNTERYT SEEVTDSRIL
CLALVYLPVE KESWIVSGTQ SGMLLVINTE DGRKRHTLEK MTDSVTCLYC NSFSKQSKQK
NFLLVGTADG NLAIFEGKTV KCKGASPLKI LNIGNVSTPL MCLSESVNLT EKNIMWGGCG
TKVFSFSDDF TIQKLIETRT NQRFSYASFC DSNIIAVVVD TALYVAKKNS PFVEVWDKKT
EKLCELIDCV HFLKEELVRV SKELKHKMSY SGRVKTLCLQ KNTALWIGTG GGHILLLDLS
TRRIIRIIHN FYDSVRVMMT AQLGSLKNVM LVLGYNRKSA EGTQHQKEIQ SCLSVWDINL
PHEVQNLEKH IEVRKELAEK MRRISVE
//
