ID A0A452IX10_9SAUR Unreviewed; 4092 AA.
AC A0A452IX10;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA-dependent protein kinase catalytic subunit {ECO:0000256|ARBA:ARBA00018077};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
OS Gopherus agassizii (Agassiz's desert tortoise).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Durocryptodira;
OC Testudinoidea; Testudinidae; Gopherus.
OX NCBI_TaxID=38772 {ECO:0000313|Ensembl:ENSGAGP00000032597.1, ECO:0000313|Proteomes:UP000291020};
RN [1] {ECO:0000313|Proteomes:UP000291020}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=28562605;
RA Tollis M., DeNardo D.F., Cornelius J.A., Dolby G.A., Edwards T.,
RA Henen B.T., Karl A.E., Murphy R.W., Kusumi K.;
RT "The Agassiz's desert tortoise genome provides a resource for the
RT conservation of a threatened species.";
RL PLoS ONE 12:e0177708-e0177708(2017).
RN [2] {ECO:0000313|Ensembl:ENSGAGP00000032597.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC {ECO:0000256|ARBA:ARBA00011031}.
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DR Ensembl; ENSGAGT00000036948.1; ENSGAGP00000032597.1; ENSGAGG00000023217.1.
DR Proteomes; UP000291020; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004677; F:DNA-dependent protein kinase activity; IEA:InterPro.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05172; PIKKc_DNA-PK; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR037706; DNA-PK_dom.
DR InterPro; IPR046804; DNA-PKcs_N.
DR InterPro; IPR046803; DNAPKcs_CC1-2.
DR InterPro; IPR012582; DNAPKcs_CC3.
DR InterPro; IPR045581; DNAPKcs_CC5.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF68; DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT; 1.
DR Pfam; PF20500; DNA-PKcs_N; 1.
DR Pfam; PF20502; DNAPKcs_CC1-2; 1.
DR Pfam; PF08163; DNAPKcs_CC3; 1.
DR Pfam; PF19704; DNAPKcs_CC5; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM01344; NUC194; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000291020};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2843..3504
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 3686..4017
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 4060..4092
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT REGION 2575..2597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4092 AA; 467978 MW; 8D761C52FCA05C5E CRC64;
QLIKEQGQEF CCFFALPTAL QTSLVFSKDN GLLVFVRRSL SIEEFRECRE EALKFLCAFL
EKIGQQVHPY AQDIKLTCIS VYTKEKAAKC KIPALELLIK LLQNLRSSFL MEELKVGEIF
NKFYGELAAR SKVPDTVLEK LYELLGVLGE VQPSDMVDNS EKLFRAFLGE LKTQMTSATR
EPKLPVVAGC LKGLTALMYN FTKSMDEDPH TSKEIFDFAV KAINPQVEFL MSIFQVSGLH
LFSMHAAQFG TFLLDNYSSL FEVLSKWCGH TNAELKKGGH SALDSFLKQI SLMVAKDAEM
HKSKLQFFME QFYDIIRKMD SSNKELSIAI RGYGLFAAPC KAINSKDVDF MYIELLQRCK
QMYLTEAETA DEHVYQLPSF LQSIASVILH IDTIPEVYTP VLEHLIVVQI DSFPQYSVKM
QSACCKSIVK IFLALAGKGP VLWSFISTVV HQGLVRIFGK DVSKSEEPTA AGEVRTGKWK
VPTYKDYLDL FRSLLNCDTM KESVLADETF LIANSPLLSL NRLLYDELIK SILKIIEKLD
LTVQKLNVSE QDENEADSAL VVPTSDPASN LRPTKPEDFV AFVNLVEFCR EILLEKHVEF
FEPWMYSFGY ELIIQSTRLP LISGFYKMLS VAMKIAKKIK YFEGVSPKSY QKCPDDPEKS
SCFALYAKFG KEVTAKMKQY KDELLASCLT FVLSLPHDII MLDIKAYIPA LQNAFKLGLS
YTPMADAGLD ALEDWSARIP KHIMQPYYKD VLPLFDGYLK TTGLADESQN NWEVRKLSRA
AQKGFNKVVI QRLKKAKTLS LEDHLSLEAV RTRVVRLLGN LGGQINRNLL TAASAEEMIK
KFVSWDTEKH LSFAVPFADM KPVIYLDLFL PRVTELALSA SDRQTKVAAC ELLHSIVMYM
LGKASQMPDG PPDSPAMYQL YKRMFPVLLR LACDVDQVTR QLYELLVMEL IHWFTNNKKF
ESQDTVALLE AILDGIVDPV DSSLRDFCGQ CVREFMKWSI KQTTPQQQEK SPVNTKSLFK
RLYSLALHPN ALKRLGAALA FNNIYREFRE ENSLVEQFVF EVLVVYLESL ALAHTDEKSL
GTVQQCCDAI DHLKRIIKHK ASSLNKAEKR RIPRGFPNAK SVCLLDLVMW LLVQCGRPQT
ECRHKSMELF YEFVPLLPGN QSSSLWLGDV LKKQGISFLI NKFEGGGNDG DNLSGILSQP
TLCDMKEPFS LRAVVQWMDM FLAVLDCYNT FIEQGMIKPN EILGTKMRSS FLRAVEFFLK
TIVLHDISAA ERCFDTGSKG NMFSPQEREE YNYSKCTIIV RIMAFVSMIL ETCQQDFWKL
LEKELLNANL IELLVRIVCD PANIGFNTAD VQVMKNLPDV SVRLIKALMK SPYKESLELS
IKERITSQSE RCKVNELYNP DARFDHVRLS SILSACRQLH KAGLLHSTLQ SQGQGLHFTV
GSKLLSVVYK SIAPGDEKKS LPSLDISSKR LADGLLQLAF AFGGQCEELV SLLLNTVMLS
VPLSGTSQRS LISFSHGEYF YSLFSETINR ELLKDLDVAI LQLMKSSVDS PKMVGTILNG
MLDQSFRERA VRKQQGMKLV TAVLRNWMRL DSWWAKDSAP ESKMAVLTLL AKVLQIDSSV
SFNTNHEAFS AVFNTYTSLL IDENLGLNLK SQAVIILPFF TNLTGERISE LKNALDQLVA
FNFPMKSDEF PKGTLRHNNY VDCTKKFLDA LELSQSPMLL QLMTEILCRD HRHFMEDLFQ
TSFKRISRRS SCEKQVVLLD TVHKMFQSEA LLSNATRQAF VDRSFLTLLL HCSLDALKDF
LSKIVLEAMD TLKTRFTKSN ESAFDTQITK KMGYYKILEV MYSRLSKEDI YSKDSRINQA
YQGSTNVEGN ELTKTLIKSC YDAFTENMAG ESQLLEKRRQ YHCAAYNCAI AVISCVFTES
KFYQGFLFTE KPEKNLFIFE NLLDLKHQYS FPVEVEVPME RKKRYITIRK EARDAVNRDS
DEPQYLPSAS YMVDSSLSEE MSQFDFSTGV QSFSYNSQDP TATSIHFRRK EPTESMDPDD
LMELEMDELN QHECMASMTT LIKHMQRNQI TPKVDEVSIV PRDLPPWMKF IHGKLGNPSV
PLNIRLFLAK LLVNTEEVFR PYARQWLGPL LQLVVSRDNG GEGIHYMVVE IVVTLLSWTS
VAAPKGNIKD EILANRLLEF LMKNVFHQKR AVFRHNLEII KTVVECWKDC LSIPYRLIFE
QFSGGDPNTK DNSVGIQLLG IVLANNLSPY DSKCEIDRVR YFQALANNMS LIRYKEVYAA
AAEVLGLILR YMAEKENIFE GPVYDCVIKQ IKQHQSTKED KFIVCLNKVA KNFPPLADRF
MNAVFFLIPK LHGVLKTYCL EVVMYRAEEI TDLFLQLKSK DFIQVMSHRD DERQRVCLDI
VYKMLSKLKP LELTELLPGV TGFISHPSPV CRERMYDILM WIYDNYRDPE SQAEEESQKV
FKLAKEMLLQ GLMDENSGLQ LIVRNFWSDE TRLPANTLDR MLALLNSLYS TKIETQFLSL
ATNFLLEMTS KSPDYSRKMF EHPLSECRFQ DFTIDSNWRY RSTVLTPMFV ETQATQSAKR
NRSQEGSLST QGSLGGQVRA TQQQYEFTPT QNVNGRSSFN WLTGSSIDTL AEYIVPSSSE
SLSSSMLFVS KRSEKSQRAT LQPLGPNFGK KRLGLPGDEV DSKAKGIDER AEILRLRRRF
LKDQEKLSLI YARKGVAEQK REKEIKSELK MKHDAQVTLY RSYRQGDLPD IQIEYSSLIT
PLQGVAQRDP PLAKQLFSSL FTGILKEMNK FKNPSEKNCI TQELLQNFNR FLSTTVSYFP
PFVACIQEMS YQHRELLDLD SATVSTSCLA SLQQPVGILL LEQALTLLSS EEPPRSKRMR
GRAELSPDVI RWIELAKLYR SVGDYDVLRG IFSGKIGTKE ITQNALLAEA RSDYAEAAKQ
YDEALSKQDW LDGEPSEAEK DFWELASLEC YNHLTEWKSL EYCSTVNIDS GQPPDLNKTW
SDPFYQETYL PYIIRSKLKL LLHGGNDQSL LTFIDEAMKI EQRKALIEMY YSQELSLLYI
LQDDFDRARY YISSGMQAFM QNYSSIDTLL HQSRLTKLQS VQALTEIQEF INFTSKPSSL
ASQASLKRLL RIWTSRYPDD KMDPMNIWDD VITNRCFFLG KIQEKLPNIQ ADDSMEVDGR
GDVGDKMETD MQEEDIHSMI RSCKFNMKMK MIESARKQNS FSVAMKLLKD LHRESKTRED
WLLRWNHSYC RFSHSRSQSQ NSPEQMLTVL KTISLLDESK SDYLSKNIVA FRNQNLLLGT
TYYILASALS KDPGCLEKIE EGKARKVLML SGERPENSEK VIAGLNKKAF QHFSTAVRKA
EEEVQSHSME HVDLTGVINA YMTLVDFCDK HLRKEEEDIS ALDLQTFPAI VVEKMIKALK
LNSRDARLKF PRLLQIIERY PAETLGLMAR EISSIPCWQF IGWISQLMAV LDKEEAVAVQ
HTVEEIANSY PQAIIYPFLI SSENYSFKDT AASHKNKEFV ARIKSKLDRG GVIQDFVHAL
EQLSNPVMLF KDWVEDVKNE LGKTQRNKNN LKEMYEGMYR NLGDLQAPGL GSFRRRFIQT
FGKDFNSHFG KGGSKLLDMK ADDFNRIAFS LLSKMKDQKE PGNLKECSPW MSDFKADFLR
NELEIPGQYD GKGKPLPEYH AKISGFDERI KVMESIRKPK RINIRGSDER EHPFLVKGGE
DLRQDQRIEQ LFDVMNIILS QDAACSQRNM QIKTYKVIPM TTRLGLIEWL ENTCTLKNFL
MESMSEEEKD DYHSRKGPRI AYSDWLSKMA GKETGIARYK TMYSRANRTE TVLSFRNREN
SVPGDLLRRG FVKMSTAPEA FLSLRSHFAS SHALMCISHW ILGIGDRHLS NFMINMETGG
MVGIDFGHAF GSATQFLQVP ELMPFRLTRQ FINLMLPMKE SGLIYSVMVH SLRAFRTEPD
LLISTMDVFV KEPSLDWQNF ELKQLKKGGT WSKEVNTAEI NWYPLQKVNC AKRKLAGANP
AVLTCDELRL GHEKSPAYKE YVAVAQGSRD HNIRAKEPED GLLEEIQVRC LIDQATDPNL
LGRVWEGWEP WM
//
