ID A0A452R4E5_URSAM Unreviewed; 1468 AA.
AC A0A452R4E5;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Alpha-2-macroglobulin {ECO:0000313|Ensembl:ENSUAMP00000013288.1};
GN Name=A2M {ECO:0000313|Ensembl:ENSUAMP00000013288.1};
OS Ursus americanus (American black bear) (Euarctos americanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae; Ursus.
OX NCBI_TaxID=9643 {ECO:0000313|Ensembl:ENSUAMP00000013288.1, ECO:0000313|Proteomes:UP000291022};
RN [1] {ECO:0000313|Proteomes:UP000291022}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Korstanje R., Srivastava A., Sarsani V.K., Sheehan S.M., Seger R.L.,
RA Barter M.E., Lindqvist C., Brody L.C., Mullikin J.C.;
RT "De novo assembly and RNA-Seq shows season-dependent expression and editing
RT in black bear kidneys.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSUAMP00000013288.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- SUBUNIT: Homotetramer; disulfide-linked.
CC {ECO:0000256|ARBA:ARBA00038769}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the protease inhibitor I39 (alpha-2-
CC macroglobulin) family. {ECO:0000256|ARBA:ARBA00010952}.
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DR Ensembl; ENSUAMT00000014903.1; ENSUAMP00000013288.1; ENSUAMG00000009280.1.
DR GeneTree; ENSGT00940000154904; -.
DR Proteomes; UP000291022; Unassembled WGS sequence.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd02897; A2M_2; 1.
DR Gene3D; 1.50.10.20; -; 1.
DR Gene3D; 2.20.130.20; -; 1.
DR Gene3D; 2.60.120.1540; -; 1.
DR Gene3D; 2.60.40.1930; -; 2.
DR Gene3D; 2.60.40.1940; -; 1.
DR Gene3D; 2.60.40.690; Alpha-macroglobulin, receptor-binding domain; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR009048; A-macroglobulin_rcpt-bd.
DR InterPro; IPR036595; A-macroglobulin_rcpt-bd_sf.
DR InterPro; IPR011625; A2M_N_BRD.
DR InterPro; IPR041813; A2M_TED.
DR InterPro; IPR047565; Alpha-macroglob_thiol-ester_cl.
DR InterPro; IPR011626; Alpha-macroglobulin_TED.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR001599; Macroglobln_a2.
DR InterPro; IPR019742; MacrogloblnA2_CS.
DR InterPro; IPR002890; MG2.
DR InterPro; IPR041555; MG3.
DR InterPro; IPR040839; MG4.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR InterPro; IPR010916; TonB_box_CS.
DR PANTHER; PTHR11412:SF165; ALPHA-2-MACROGLOBULIN; 1.
DR PANTHER; PTHR11412; MACROGLOBULIN / COMPLEMENT; 1.
DR Pfam; PF00207; A2M; 1.
DR Pfam; PF07703; A2M_BRD; 1.
DR Pfam; PF07677; A2M_recep; 1.
DR Pfam; PF01835; MG2; 1.
DR Pfam; PF17791; MG3; 1.
DR Pfam; PF17789; MG4; 1.
DR Pfam; PF07678; TED_complement; 1.
DR SMART; SM01360; A2M; 1.
DR SMART; SM01359; A2M_N_2; 1.
DR SMART; SM01361; A2M_recep; 1.
DR SMART; SM01419; Thiol-ester_cl; 1.
DR SUPFAM; SSF49410; Alpha-macroglobulin receptor domain; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 1.
DR PROSITE; PS00477; ALPHA_2_MACROGLOBULIN; 1.
DR PROSITE; PS00430; TONB_DEPENDENT_REC_1; 1.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Protease inhibitor {ECO:0000256|ARBA:ARBA00022690};
KW Reference proteome {ECO:0000313|Proteomes:UP000291022};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Serine protease inhibitor {ECO:0000256|ARBA:ARBA00022900};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Thioester bond {ECO:0000256|ARBA:ARBA00022966}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..1468
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5019411444"
FT DOMAIN 456..604
FT /note="Alpha-2-macroglobulin bait region"
FT /evidence="ECO:0000259|SMART:SM01359"
FT DOMAIN 737..827
FT /note="Alpha-2-macroglobulin"
FT /evidence="ECO:0000259|SMART:SM01360"
FT DOMAIN 1373..1460
FT /note="Alpha-macroglobulin receptor-binding"
FT /evidence="ECO:0000259|SMART:SM01361"
SQ SEQUENCE 1468 AA; 163387 MW; 9A9BB41CFE40DE2E CRC64;
MGKNKLFHPN LILLLLVFLP ADASVSGKPQ YMVLVPSLLH SETPEKGCLL LSYLNETVTV
SASLESLREN RSLFTDLVVE KDLFHCVSFT VPRSPSNEEV MFLTIQVKGS TQEFKRRTTV
VVKNQESLVF VQTDKPIYKP GQTVKFRIVS LDENFHPLNE LVSLQDPKGN RITQWQKLKL
ENGLKQLSFA LSSEPFQGSY KVVVRKESGG ITEHAFTVEE FVLPKFEVQI TMPRIITILE
EDVNVSVCGL YTYGKPVPGH MTMRVCRKYS NPSNCYSGES QAVCEKFSHQ LNSQGCFSQQ
VKTKIFQMKR QGYEMKLEAE AKIQEEGTEV ELTGKGSTEI TRTITKLSFV KVDPYFRQGI
PFFGQVQLLD GKGVPMPDEL VFITANEANY NSNATTDEHG LVQFSINTTD ITGTSLTVRV
KHKDSSPCYG YQWLLAEHEE AHHTANLVFS LSKSFVRLEP LPRELPCGQT QTVQAHYILN
ARVLQELNEL VFYYLIMAKG GVVLNGAHML PVKQGDMKGH FSVSIPVESD IAPVARMLIY
AILPDGEVIG DSAKYKVENC LANKVDLSFR PTQSLPASHA HLQVSASPQS LCALRAVDQS
VLLMKPEAEL SAASVYNLLP VKDLSGFPGS LNRQEEDDRD CINRHNVYIN GVMYSPVSNT
NEKDMYSFLQ DMGLKVFTNT KIRKPKVCSQ PEHEAVYSSR RLFTSPAHLE LILRLQDSMV
HVSEPLTETV RKYFPETWIW DLVAVDSSGV AEVGVIVPDT ITEWKAGALC LSNDTGLGLS
LPASLRAFQP FFVELTMPYS VIRGEAFTLK ATVLNYLPKC IQVGVQLDPN PAFLVQEKEQ
ESHCICGNGR HTVSWAVTPK ALGNVNFTVS AEALESQEPC GTEVAVVPEF GKKDTIIKSL
LVEPEGLEKE VTFNSLLCPS GVEVSEQLPL KLPENVVEES ARASVSVLGD LLGSAMHNIQ
NLLRMPYGCG EQNMVLFAPN IYVLNYLNET HQLTPEVMSK AVGYLNTGYQ RQLKYKHHDG
SYSTFGENYG KSEGNTWLTA FVLKSFAQAR AYIFIDEAHI TQALTWLSQK QKDSGCFRSS
GSLLNNAIKG GVEDEVTLSA YITIALLEIP LPATHPVVRN ALFCLESAWK SAKERPHGKH
VYTKALLAYA FALAGNQDKR RETLDLLHGE AVKEDNSVHW ERPQKPRAPV GHFYQPPAPS
AEVEMTSYVL LAYLTAQPAP TSEELTSAAR IVKWITKQQN SQGGFSSTQD TVVALQALSR
YGTATFTRTG KPAQVTVQYS GTFVTKFQVD DDNRLLLQQT SLPKVPEEYT MTVTGEGCVY
LQTSLKYNIL PEKAEFPFAL EVQTVPETCD GPKAHTSFQI SLNVSYIGSR PVSNMVIIDA
KMVSGFIPLK PTVKMLERSN HVSRTEVSNN HVLIYLDKVT NQTLSLSFRV LQDVPVRELK
PAIVKVYDYY ETDEFAIAEY SAPCSQGK
//
