ID A0A452S1F1_URSAM Unreviewed; 909 AA.
AC A0A452S1F1;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=Solute carrier family 12 member 1 {ECO:0000313|Ensembl:ENSUAMP00000025741.1};
GN Name=SLC12A1 {ECO:0000313|Ensembl:ENSUAMP00000025741.1};
OS Ursus americanus (American black bear) (Euarctos americanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae; Ursus.
OX NCBI_TaxID=9643 {ECO:0000313|Ensembl:ENSUAMP00000025741.1, ECO:0000313|Proteomes:UP000291022};
RN [1] {ECO:0000313|Proteomes:UP000291022}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Korstanje R., Srivastava A., Sarsani V.K., Sheehan S.M., Seger R.L.,
RA Barter M.E., Lindqvist C., Brody L.C., Mullikin J.C.;
RT "De novo assembly and RNA-Seq shows season-dependent expression and editing
RT in black bear kidneys.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSUAMP00000025741.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR AlphaFoldDB; A0A452S1F1; -.
DR Ensembl; ENSUAMT00000028728.1; ENSUAMP00000025741.1; ENSUAMG00000018211.1.
DR GeneTree; ENSGT00940000158030; -.
DR Proteomes; UP000291022; Unassembled WGS sequence.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IEA:UniProt.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002445; Slc12a1.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF93; SOLUTE CARRIER FAMILY 12 MEMBER 1; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01209; NAKCLTRSPRT2.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000291022};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 184..203
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 210..240
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 260..281
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 302..321
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 327..348
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 380..399
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 546..565
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 571..592
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 604..624
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 630..645
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 90..157
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 183..686
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 695..830
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 29..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 149..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..50
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 909 AA; 99270 MW; 17706F798DA0E9D4 CRC64;
MSLNNSSTVF LDSVPGNSNR FQVNVVNESH ESSAAVDDNA DPPHYEETSF GDETQNRFRI
SFRPGNRECY DNFLQNGENA KTDASFHAYD SHTNTYYLQT FGHNTVDAVP KIEYYRNTGS
ISGPKVNRPS LLDLHEQLAK NIAVAPGSAD RVANGDGMPG DEEAEPKEGE DKAGAVKFGW
VKGVLVRCML NIWGVMLFIR LSWIVGEAGI GLGVIIIGLA VTVTGITGLS TSAIATNGYV
RGGGAYYLIS RSLGPEFGGS IGLIFAFANA VAVAMYVVGF AETVVDLLKE SNSMMVDPTN
DIRIIGSITV VILLGISVAG MEWEAKAQVI LLVILLIAIA NFFIGTVIPS NNEKKSRGFF
NYQASIFAEN FGPSFTKGEG FFSVFAIFFP AATGILAGAN ISGDLEDPQD AIPRGTMLAI
FITTVAYIGV AICVGACVVR DATGNMNDTI ISGMNCNGSA ACGLGYDFSR CRHEPCQYGL
MNNFQVMSMV SGFGPLITAG IFSATLSSAL ASLVSAPKVF QALCKDNIYK ALQFFAKGYG
KNNEPLRGYF LTFVIAMAFI LIAELNTIAP IISNFFLASY ALINFSCFHA SYAKSPGWRP
AYGIYNMWVS LFGAILCCAV MFVINWWAAV ITYVIEFFLY IYVTYKKPDV NWGSSTQALS
YVSALDNALE LTTVEDHVKN FRPQCIVLTG GPMTRPALLD ITHAFTKNSG LCICCEVFVG
PRKLCVKEMN SGMAKKQAWL IKNKIKAFYA AVAADCFRDG VRSLLQASGL GRMKPNTLVI
GYKKNWRKAP LTEIENYVGI IHDAFDFEIG VVIVRIGHGF DISQVLQVQG MYFLYSNDKY
LLSTYSMPGL FLSAGDRVVY NTDKPPSDGA CLFFNCQINF CKMFEKIKIN SFLEKARILF
LRVYDLVCS
//
