ID A0A452S720_URSAM Unreviewed; 4749 AA.
AC A0A452S720;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=RCR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012249};
DE EC=2.3.2.33 {ECO:0000256|ARBA:ARBA00012249};
GN Name=MYCBP2 {ECO:0000313|Ensembl:ENSUAMP00000027977.1};
OS Ursus americanus (American black bear) (Euarctos americanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae; Ursus.
OX NCBI_TaxID=9643 {ECO:0000313|Ensembl:ENSUAMP00000027977.1, ECO:0000313|Proteomes:UP000291022};
RN [1] {ECO:0000313|Proteomes:UP000291022}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Korstanje R., Srivastava A., Sarsani V.K., Sheehan S.M., Seger R.L.,
RA Barter M.E., Lindqvist C., Brody L.C., Mullikin J.C.;
RT "De novo assembly and RNA-Seq shows season-dependent expression and editing
RT in black bear kidneys.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSUAMP00000027977.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine.;
CC EC=2.3.2.33; Evidence={ECO:0000256|ARBA:ARBA00000333};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Cell projection, axon
CC {ECO:0000256|ARBA:ARBA00004489}.
CC -!- SIMILARITY: Belongs to the RING-Cys relay (RCR) family.
CC {ECO:0000256|ARBA:ARBA00005415}.
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DR STRING; 9643.ENSUAMP00000027977; -.
DR Ensembl; ENSUAMT00000031256.1; ENSUAMP00000027977.1; ENSUAMG00000020804.1.
DR GeneTree; ENSGT00940000155756; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000291022; Unassembled WGS sequence.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:Ensembl.
DR GO; GO:0031267; F:small GTPase binding; IEA:Ensembl.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:Ensembl.
DR GO; GO:0008270; F:zinc ion binding; IEA:Ensembl.
DR GO; GO:0032922; P:circadian regulation of gene expression; IEA:Ensembl.
DR GO; GO:0031398; P:positive regulation of protein ubiquitination; IEA:Ensembl.
DR GO; GO:0070936; P:protein K48-linked ubiquitination; IEA:Ensembl.
DR CDD; cd19799; Bbox2_MYCBP2; 1.
DR CDD; cd16463; RING-H2_PHR; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.60.120.820; PHR domain; 2.
DR Gene3D; 2.130.10.30; Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR004939; APC_su10/DOC_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR012983; PHR.
DR InterPro; IPR038648; PHR_sf.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR45943; E3 UBIQUITIN-PROTEIN LIGASE MYCBP2; 1.
DR PANTHER; PTHR45943:SF1; E3 UBIQUITIN-PROTEIN LIGASE MYCBP2; 1.
DR Pfam; PF03256; ANAPC10; 1.
DR Pfam; PF08005; PHR; 2.
DR Pfam; PF00415; RCC1; 1.
DR Pfam; PF13540; RCC1_2; 1.
DR SMART; SM01337; APC10; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF50985; RCC1/BLIP-II; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51284; DOC; 1.
DR PROSITE; PS00626; RCC1_2; 2.
DR PROSITE; PS50012; RCC1_3; 3.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000291022};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT REPEAT 601..656
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 959..1009
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1010..1067
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT DOMAIN 3789..3967
FT /note="DOC"
FT /evidence="ECO:0000259|PROSITE:PS51284"
FT DOMAIN 4499..4550
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 87..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 170..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 609..628
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 898..928
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2780..3003
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3051..3092
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3676..3700
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..121
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 614..628
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 908..924
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2780..2807
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2808..2829
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2855..2928
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2932..2962
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4749 AA; 521467 MW; F187DD4180D87246 CRC64;
MMMCSATASP AAASSGPGGD GFFPAATISS SPAPGALFMP VPEGSVAAVG LGLGLPAADS
RGHYQLLLSG RALADRYRRI YTAALSDRDQ GGSSAGHPAS RNKKILNKKK LKRKQKSKSK
VKTRSKSENL ESTVIIPDIK LHSNPSAFNI YCNVRHCVLE WQKKETSLAA ASKNSVQSGE
SDSDEEEESK EPPIKLPKII EVGLCEVFEL IKETRFSHPS LCLRSLQALL NVLQGQQPEG
LQSEPPEVLE SLFQLLLEIT VRSTGMNDST GQSLTALSCA CLFSLVASWG ETGRTLQAIS
AILTNNGSHA CQTIQVPTIL NSLQRSVQAV LVGKIQIQDW FSNGIKKAAL MHKWPLKEIT
VDEDDQCLLQ NDGFFLYLLC KDGLYKIGSG YSGTVRGHIY NSTSRIRNRK EKKSWLGYAQ
GYLLYRDVNN HSMTAIRISP ETLEQDGTVM LPDCHTEGQN ILFTDGEYIN QIAASRDDGF
VVRIFATSTE PVLQQELQLK LARKCLHACG ISLFDLEKDL HIISTGFDEE SAILGAGREF
ALMKTASGKI YYTGKYQSLG IKQGGPSAGK WVELPITKSP KIVHFSVGHD GSHALLVAED
GSIFFTGSAS KGEDGESTKS RRQSKPYKPK KIIKMEGKIV VYTACNNGSS SVISKDGELY
MFGKDAIYSD SSSLVTDLKG HFVTQVAMGK AHTCVLMKNG EVWTFGVNNK GQCGRDTGAM
NQGGKGFGVE NMATAMDEDL EEELDEKDEK SMMCPPGMHK WKLEQCMVCT VCGDCTGYGA
SCVSSGRPDR VPGGICGCGS GESGCAVCGC CKACARELDG QEARQRGILD AVKEMIPLDL
LLAVPVPGVN IEEHLQLRQE EKRQRVIRRH RLEEGRGPLV FAGPIFMNHR EQALARLRSH
PAQLKHKRDK HKDGSGERGE KDASKITTYP PGSVRFDCEL RAVQVSCGFH HSVVLMENGD
VYTFGYGQHG QLGHGDVNSR GCPTLVQALP GPSTQVTAGS NHTAVLLMDG QVFTFGSFSK
GQLGRPILDV PYWNAKPAPM PNIGSKYGRK ATWIGASGDQ TFLRIDEALI NSHVLATSEI
FASKHIIGLV PASISEPPPF KCLLINKVDG SCKTFNDSEQ EDLQGFGVCL DPVYDVIWRF
RPNTRELWCY NAVVADARLP SAADMQSRCS ILSPELALPT GSRALTTRSH AALHILGCLD
TLAAMQDLKM GVASTEEETQ AVMKVYSKED YSVVNRFESH GGGWGYSAHS VEAIRFSADT
DILLGGLGLF GGRGEYTAKI KLFELGPDGG DHETDGDLLA ETDVLAYDCA AREKYAMMFD
EPVLLQAGWW YVAWARVSGP SSDCGSHGQA SITTDDGVVF QFKSSKKSNN GTDVNAGQIP
QLLYRLPTSD GSASKGKQQT SEPVHILKRS FARTVSVECF ESLLSILHWS WTTLVLGVEE
LRGLKGFQFT ATLLDLERLR FVGTCCLRLL RVYTCEIYPV SATGKAVVEE TSKLAECIGK
TRTLLRKILS EGVDHCMVKL DNDPQGYLSQ PLSLLEAVLQ ECHNTFTACF HSFYPTPALQ
WACLCDLLNC LDQDIQEANF KTSSSRLLAA VMSALCHTSV KLTSIFPIAY DGEVLLRSIV
KQVSTENDST LVHRFPLLVA HMEKLSQSEE NISGMTSFRE VLEKMLVIVV LPVRNSLRRE
NELFSSHLVS NTCGLLASIV SELTASALGS EVDGLNSLHS VKASANRFTK TSQGRSWNTG
NGSPDAICFS VDKPGIVVVG FSVYGGGGIH EYELEVLVDD SEHVGDSTHS HRWTSLELVK
GTYTTDDSPS DIAEIRLDKV VPLKENVKYA VRLRNYGSRT ANGDGGMTTV QCPDGVTFTF
STCSLSSNGT NQTRGQIPQI LYYRSEFDGD LQSQLLSKAN EEDKNCSRAL SVVSTVVRAA
KDLLHRALAV DADDIPELLS SSSLFSMLLP LIIAYIGPVA AAIPKVAVEV FGLVQQLLPS
VAILNQKYAP PAFNPNQSTD STTGNQPEQG LSACTTSNHY AVIESEHPYK PACVMHYKVT
FPECVRWMTI EFDPQCGTAQ SEDVLRLLIP VRTVQNSGYG PKLTSVLENL NSWIELKKFS
GSSGWPTMVL VLPGNEALFS LETASDYVKD DKASFYGFKC FAIGYEFSPG PDEGVIQLEK
ELANLGGVCA AALMKKDLAL PIGNELEEDL EILEEAALQV CKTHSGILGK GLALSHSPTI
LEALEGNLPL QIQSNEQSFL DDFIACVPGS SGGRLARWLQ PDSYADPQKT SLILNKDDIR
CGWPTTITVQ TKDQYGDVVH VPNMKVEVKA VPVSQKKTSL QQEQVKKPQR IPGSPAVAAA
PSNTDMTFGG LASPKLDVSY EPMIVKEARY IAITMMKVIL NFLISILFLF RPSENMLIRV
NNDGTYCANW TPGAIGLYTI HVTIDGIEID AGLEVKVKDP PKGMIPPGTQ LVKPKTEPQP
NKVRKFVAKD SAGLRIRSHP SLQSEQIGIV KVNGTITFID EIHNDDGVWL RLNDETIKKY
VPNMNGYTEA WCLSFNQHLG KSLLVPVDSI FNASQGVRDL DVFSWTSKAF FPQEPKTNTD
DFFKDINSCC PQEATMQEQE MPFLRGGPGM YKVVKTGPSG HNIRSCPNLR GIPIGMLVLG
NKVKAVGEVT NSEGAWVQLD KNSMVEFCES DEGEAWSLAR DRGGNQYLRH EDEQVLLDQN
SQTPPPSPFS VQAFNKGASC SAQGFDYGLG NNKGDQLSAI LNSIQSRPNL PAPSIFDQAA
KPPSSLVHSP FVFGQPLSFQ QPQLQSDRGT ISTSSRPVST SGKSELSSKH SRSLKPDGRM
SRTTVDQKKP RGTEGLSASE SLMLKSDAAK LRSDSHSRSL SPNHNTLQTL KSDGRMSSSF
RAESPGPGSR SSSPKPKTLP ANRSSPSGAS SPRSSSPHDK NLPQKSTAPV KTKLDPPRER
SKSDSYTLDP DTLRKKKMPL TEPLRGRSTS PKPKPVPKDS KDSPGSENRA PSPHVVQENL
HSEVVEVCTS STLKTNSLTD STCDESSEFK SVDESSNKVH FSIGKAPLKD EQEMRASPKI
SRKCANRHTR PKKEKSSFLF KGDGSKPLEP AKQAMSPSVA ECARAVFASF LWHEGIVHDA
MACSSFLKFN PELSKEHAPI RNSLNSQQPT EEKETKLKNR HSLEISSALN MFNIAPHGPD
ISKMGSINKN KVLSMLKEPP LHEKCEDGKT EATFEMSMHH PMKSKSPLPL TLQHLVAFWE
DISLATIKAA SQNMIFPSPG SCAVLKKKEC EKENKKAKKD KKKKEKTEVR PRGNLFGEMA
QLAVGGPEKD TICELCGESH PYPVTYHMRQ AHPGCGRYAG GQGYNSIGHF CGGWAGNCGD
GGIGGSTWYL VCDRCREKYL REKQAAAREK VKQSRRKPMQ VKTPRALPTM EAHQVIKANA
LFLLSLSSAA EPSILCYHPA KPFQSHLPSV KEGISEDLPV KMPCLYLQTL ARHHHENFVG
YQDDNLFQDE MRYLRSTSVP APYISVTPDA SPNVFEEPES NMKSMPPSLE TSPITDTDLA
KRTVFQRSYS VVASEYDKQH SILPARVKAI PRRRVNSGDT VGSSLLRHPS PELSRLISAH
SSLSKGERNF QWPVLAFVIQ HHDLEGLEIA MKQALRKSAC RVFAMEAFNW LLCNVIQTTS
LHDILWHFVA SLTPAPVEPE EEEDEENKTN KENAEQEKDT RVCEHPLSDI VIAGEAAHPL
PHTFHRLLQT ISDLMMSLPS GSSLQQMALR CWSLKFKQSD HQFLHQSNVF HHINNILSKS
DDGDSEESFS ISIQSGFEAM SQELCIVMCL KDLTSIVDIK TSSRPAMIGS LTDGSTETFW
ESGDEDKNKT KNITINCVKG INARYVSVHV DNSRDLGNKV TSMTFLTGKA VEDLCRIKQV
DLDSRHIGWV TSELPGGDNH IIKIELKGPE NTLRVRQVKV LGWKDGESTK IAGQISASVA
QQRNCEAETL RVFRLITSQV FGKLISGDAE PTPEQEEKAL LSSPEGEEKV YNATSDADLK
EHMVGIIFSR SKLTNLQKQV CAHIVQAIRM EATRVREEWE HAISSKENAN SQPNDEDASS
DAYCFELLSM VLALSGSNVG RQYLAQQLTL LQDLFSLLHT ASPRVQRQVT SLLRRVLPEV
TPNRLASIIG VKALPPADIS DIIHSTEKGD WNKLGILDMF LGCIAKALTV QLKAKGTTIT
GTAGTTVGKG VTTVTLPMIF NSSYVRRGES HWWMKGSTPT QISEIIIKLI KDMAAGHLSE
AWSRVTKNAI AETIIALTKM EEEFRSPVRC IATTRLWLAL ASLCVLDQDH VDRLSSGRWM
GKDGQQKQMP MCDNHDDGET AAIILCNVCG NLCTDCDRFL HLHRRTKTHQ RQVFKEEEEA
IKVDLHEGCG RTKLFWLMAL ADSKTMKAMV EFREHTGKPT TSSSEACRFC GSRSGTELSA
VGSVCSDADC QEYAKIACSK THPCGHPCGG VKNEEHCLPC LHGCDKSATT LKQDADDMCM
ICFTEALSAA PAIQLDCSHV FHLQCCRRVL ENRWLGPRIT FGFISCPICK NKINHIVLKD
LLDPIKELYE DVRRKALMRL EYEGLHKSEA ITTPGVRFYN DPAGYAMNRY AYYVCYKCRK
AYFGGEARCD AEAGQGDDYD PRELICGACS DVSRAQMCPK HGTDFLEYKC RYCCSVAVFF
CFGTTHFCNA CHDDFQRMTS IPKEELPHCP AGPKGKQLEG TECPLHVVHP PTGEEFALGC
GVCRNAHTF
//
