ID A0A452S773_URSAM Unreviewed; 4507 AA.
AC A0A452S773;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=RCR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012249};
DE EC=2.3.2.33 {ECO:0000256|ARBA:ARBA00012249};
GN Name=MYCBP2 {ECO:0000313|Ensembl:ENSUAMP00000028009.1};
OS Ursus americanus (American black bear) (Euarctos americanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae; Ursus.
OX NCBI_TaxID=9643 {ECO:0000313|Ensembl:ENSUAMP00000028009.1, ECO:0000313|Proteomes:UP000291022};
RN [1] {ECO:0000313|Proteomes:UP000291022}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Korstanje R., Srivastava A., Sarsani V.K., Sheehan S.M., Seger R.L.,
RA Barter M.E., Lindqvist C., Brody L.C., Mullikin J.C.;
RT "De novo assembly and RNA-Seq shows season-dependent expression and editing
RT in black bear kidneys.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSUAMP00000028009.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine.;
CC EC=2.3.2.33; Evidence={ECO:0000256|ARBA:ARBA00000333};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Cell projection, axon
CC {ECO:0000256|ARBA:ARBA00004489}.
CC -!- SIMILARITY: Belongs to the RING-Cys relay (RCR) family.
CC {ECO:0000256|ARBA:ARBA00005415}.
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DR Ensembl; ENSUAMT00000031292.1; ENSUAMP00000028009.1; ENSUAMG00000020804.1.
DR GeneTree; ENSGT00940000155756; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000291022; Unassembled WGS sequence.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd19799; Bbox2_MYCBP2; 1.
DR CDD; cd16463; RING-H2_PHR; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.60.120.820; PHR domain; 2.
DR Gene3D; 2.130.10.30; Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR004939; APC_su10/DOC_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR012983; PHR.
DR InterPro; IPR038648; PHR_sf.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR45943; E3 UBIQUITIN-PROTEIN LIGASE MYCBP2; 1.
DR PANTHER; PTHR45943:SF1; E3 UBIQUITIN-PROTEIN LIGASE MYCBP2; 1.
DR Pfam; PF03256; ANAPC10; 1.
DR Pfam; PF08005; PHR; 2.
DR Pfam; PF00415; RCC1; 1.
DR Pfam; PF13540; RCC1_2; 1.
DR SMART; SM01337; APC10; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF50985; RCC1/BLIP-II; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51284; DOC; 1.
DR PROSITE; PS00626; RCC1_2; 2.
DR PROSITE; PS50012; RCC1_3; 3.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000291022};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT REPEAT 573..628
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 928..978
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 979..1036
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT DOMAIN 3550..3728
FT /note="DOC"
FT /evidence="ECO:0000259|PROSITE:PS51284"
FT DOMAIN 4257..4308
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 87..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 581..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 867..897
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2567..2763
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2811..2852
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3437..3461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3747..3774
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..110
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 586..600
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 877..893
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2570..2589
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2615..2688
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2692..2722
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3751..3774
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4507 AA; 494960 MW; 6827B3DCC1EFB4B9 CRC64;
MCSATASPAA ASSGPGGDGF FPAATISSSP APGALFMPVP EGSVAAVGLG LGLPAADSRG
HYQLLLSGRA LADRYRRIYT AALSDRDQGG SSKILNKKKL KRKQKSKSKV KTRSKSENLE
STVIIPDIKL HSNPSAFNIY CNVRHCVLEW QKKETSLAAA SKNSVQSGES DSDEEEESKE
PPIKLPKIIE VGLCEVFELI KETRFSHPSL CLRSLQALLN VLQGQQPEGL QSEPPEVLES
LFQLLLEITV RSTGMNDSTG QSLTALSCAC LFSLVASWGE TGRTLQAISA ILTNNGSHAC
QTIQVPTILN SLQRSVQAVL VGKIQIQDWF SNGIKKAALM HKWPLKEITV DEDDQCLLQN
DGFFLYLLCK DGLYKIGSGY SGTVRGHIYN STSRIRNRKE KKSWLGYAQG YLLYRDVNNH
SMTAIRISPE TLEQDGTSFV FFTDDFKRCD GFVVRIFATS TEPVLQQELQ LKLARKCLHA
CGISLFDLEK DLHIISTGFD EESAILGAGR EFALMKTASG KIYYTGKYQS LGIKQGGPSA
GKWVELPITK SPKIVHFSVG HDGSHALLVA EDGSIFFTGS ASKGEDGEST KSRRQSKPYK
PKKIIKMEGK IVVYTACNNG SSSVISKDGE LYMFGKDAIY SDSSSLVTDL KGHFVTQVAM
GKAHTCVLMK NGEVWTFGVN NKGQCGRDTG AMNQGGKGFG VENMATAMDE DLEEELDEKD
EKSMMCPPGM HKWKLEQCMV CTVCGDCTGY GASCVSSGRP DRVPGGICGC GSGESGCAVC
GCCKACAREL DGQEARQRGI LDAVKEMIPL DLLLVPGVNI EEHLQLRQEE KRQRVIRRHR
LEEGRGPLVF AGPIFMNHRE QALARLRSHP AQLKHKRDKH KDGSGERGEK DASKITTYPP
GSVRFDCELR AVQVSCGFHH SVVLMENGDV YTFGYGQHGQ LGHGDVNSRG CPTLVQALPG
PSTQVTAGSN HTAVLLMDGQ VFTFGSFSKG QLGRPILDVP YWNAKPAPMP NIGSKYGRKA
TWIGASGDQT FLRIDEALIN SHVLATSEIF ASKHIIGLVP ASISEPPPFK CLLINKVDGS
CKTFNDSEQE DLQGFGVCLD PVYDVIWRFR PNTRELWCYN AVVADARLPS AADMQSRCSI
LSPELALPTG SRALTTRSHA ALHILGCLDT LAAMQDLKMG VASTEEETQA VMKVYSKEDY
SVVNRFESHG GGWGYSAHSV EAIRFSADTD ILLGGLGLFG GRGEYTAKIK LFELGPDGGD
HETDGDLLAE TDVLAYDCAA REKYAMMFDE PVLLQAGWWY VAWARVSGPS SDCGSHGQAS
ITTDDGVVFQ FKSSKKSNNG TDVNAGQIPQ LLYRLPTSDG SASKGKQQTS EPVHILKRSF
ARTVSVECFE SLLSILHWSW TTLVLGVEEL RGLKGFQFTA TLLDLERLRF VGTCCLRLLR
VYTCEIYPVS ATGKAVVEET SKLAECIGKT RTLLRKILSE GVDHCMVKLD NDPQGYLSQP
LSLLEAVLQE CHNTFTACFH SFYPTPALQW ACLCDLLNCL DQDIQEANFK TSSSRLLAAV
MSALCHTSVK LTSIFPIAYD GEVLLRSIVK QVSTENDSTL VHRFPLLVAH MEKLSQSEEN
ISGMTSFREV LEKMLVIVVL PVRNSLRREN ELFSSHLVSN TCGLLASIVS ELTASALGSE
VDGLNSLHSV KASANRFTKT SQGRSWNTGN GSPDAICFSV DKPGIVVVGF SVYGGGGIHE
YELEVLVDDS EHVGDSTHSH RWTSLELVKG TYTTDDSPSD IAEIRLDKVV PLKENVKYAV
RLRNYGSRTA NGDGGMTTVQ CPDGVTFTFS TCSLSSNGTN QTRGQIPQIL YYRSEFDGDL
QSQLLSKANE EDKNCSRALS VVSTVVRAAK DLLHRALAVD ADDIPELLSS SSLFSMLLPL
IIAYIGPVAA AIPKVAVEVF GLVQQLLPSV AILNQKYAPP AFNPNQSTDS TTGNQPEQGL
SACTTSNHYA VIESEHPYKP ACVMHYKVGV LASIGYGPKL TSVLENLNSW IELKKFSGSS
GWPTMVLVLP GNEALFSLET ASDYVKDDKA SFYGFKCFAI GYEFSPGPDE GVIQLEKELA
NLGGVCAAAL MKKDLALPIG KSGQVCKTHS GILGKGLALS HSPTILEALE GNLPLQIQSN
EQSFLDDFIA CVPGSSGGRL ARWLQPDSYA DPQKTSLILN KDDIRCGWPT TITVQTKDQY
GDVVHVPNMK VEVKAVPVSQ KKTSLQQEQV KKPQRIPGSP AVAAAPSNTD MTFGGLASPK
LDVSYEPMIV KEARYIAITM MKVILNFLIS ILFLFRPSEN MLIRVNNDGT YCANWTPGAI
GLYTIHVTID GIEIDAGLEV KVKDPPKGMI PPGTQLVKPK TEPQPNKVRK FVAKDSAGLR
IRSHPSLQSE QIGIVKVNGT ITFIDEIHND DGVWLRLNDE TIKKYVPNMN GYTEAWCLSF
NQHLGKSLLV PVDVTNSEGA WVQLDKNSMV EFCESDEGEA WSLARDRGGN QYLRHEDEQV
LLDQNSQTPP PSPFSVQAFN KGASCSAQGF DYGLGNNKGR CLKVKLSSKH SRSLKPDGRM
SRTTVDQKKP RGTEGLSASE SLMLKSDAAK LRSDSHSRSL SPNHNTLQTL KSDGRMSSSF
RAESPGPGSR SSSPKPKTLP ANRSSPSGAS SPRSSSPHDK NLPQKSTAPV KTKLDPPRER
SKSDSYTLDP DTLRKKKMPL TEPLRGRSTS PKPKPVPKDS KDSPGSENRA PSPHVVQENL
HSEVVEVCTS STLKTNSLTD STCDESSEFK SVDESSNKVH FSIGKAPLKD EQEMRASPKI
SRKCANRHTR PKKEKSSFLF KGDGSKPLEP AKQAMSPSVA ECARAVFASF LWHEGIVHDA
MACSSFLKFN PELSKEHAPI RNSLNSQQPT EEKETKLKNR HSLEISSALN MFNIAPHGPD
ISKMGSINKN KVLSMLKEPP LHEKCEDGKT EATFEMSMHH PMKSKSPLPL TLQHLVAFWE
DISLATIKAA SQNMIFPSPG SCAVLKKKEC EKENKKAKKD KKKKEKTEVR PRGNLFGEMA
QLAVGGPEKD TICELCGESH PYPVTYHMRQ AHPGCGRYAG GQGYNSIGHF CGGWAGNCGD
GGIGGSTWYL VCDRCREKYL REKQAAAREK VKQSRRKPMQ VKTPRALPTM EAHQVIKANA
LFLLSLSSAA EPSILCYHPA KPFQSHLPSV KEGISEDLPV KMPCLYLQTL ARHHHENFVG
YQDDNLFQDE MRYLRSTSVP APYISVTPDA SPNVFEEPES NMKSMPPSLE TSPITDTDLA
KRTVFQRSYS VVASEYDKQH SILPARVKAI PRRRVNSGDT EVGSSLLRHP SPELSRLISA
HSSLSKGERN FQWPVLAFVI QHHDLEGLEI AMKQALRKSA CRVFAMEAFN WLLCNVIQTT
SLHDILWHFV ASLTPAPVEP EEEEDEENKT NKENAEQEKD TRVCEHPLSD IVIAGEAAHP
LPHTFHRLLQ TISDLMMSLP SGSSLQQMAL RCWSLKFKQS DHQFLHQSNV FHHINNILSK
SDDGDSEESF SISIQSGFEA MSQELCIVMC LKDLTSIVDI KTSSRPAMIG SLTDGSTETF
WESGDEDKNK TKNITINCVK GINARYVSVH VDNSRDLGNK VTSMTFLTGK AVEDLCRIKQ
VDLDSRHIGW VTSELPGGDN HIIKIELKGP ENTLRVRQVK VLGWKDGEST KIAGQISASV
AQQRNCEAET LRVFRLITSQ VFGKLISGDA EPTPEQEEKA LLSSPEGEEK ATSDADLKEH
MVGIIFSRSK LTNLQKQVCA HIVQAIRMEA TRVREEWEHA ISSKENANSQ PNDEDASSDA
YCFELLSMVL ALSGSNVGRQ YLAQQLTLLQ DLFSLLHTAS PRVQRQVTSL LRRVLPEVTP
NRLASIIGVK ALPPADISDI IHSTEKGDWN KLGILDMFLG CIAKALTVQL KAKGTTITGT
AGTTVGKGVT TVTLPMIFNS SYVRRGESHW WMKGSTPTQI SEIIIKLIKD MAAGHLSEAW
SRVTKNAIAE TIIALTKMEE EFRSPVRCIA TTRLWLALAS LCVLDQDHVD RLSSGRWMGK
DGQQKQMPMC DNHDDGETAA IILCNVCGNL CTDCDRFLHL HRRTKTHQRQ VFKEEEEAIK
VDLHEGCGRT KLFWLMALAD SKTMKAMVEF REHTGKPTTS SSEACRFCGS RSGTELSAVG
SVCSDADCQE YAKIACSKTH PCGHPCGGVK NEEHCLPCLH GCDKSATTLK QDADDMCMIC
FTEALSAAPA IQLDCSHVFH LQCCRRVLEN RWLGPRITFG FISCPICKNK INHIVLKDLL
DPIKELYEDV RRKALMRLEY EGLHKSEAIT TPGVRFYNDP AGYAMNRYAY YVCYKCRKAY
FGGEARCDAE AGQGDDYDPR ELICGACSDV SRAQMCPKHG TDFLEYKCRY CCSVAVFFCF
GTTHFCNACH DDFQRMTSIP KEELPHCPAG PKGKQLEGTE CPLHVVHPPT GEEFALGCGV
CRNAHTF
//
