ID A0A453K5Y4_AEGTS Unreviewed; 3446 AA.
AC A0A453K5Y4;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
OS Aegilops tauschii subsp. strangulata (Goatgrass).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Aegilops.
OX NCBI_TaxID=200361 {ECO:0000313|EnsemblPlants:AET5Gv20304400.4, ECO:0000313|Proteomes:UP000015105};
RN [1] {ECO:0000313|Proteomes:UP000015105}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. AL8/78 {ECO:0000313|Proteomes:UP000015105};
RX PubMed=25035499; DOI=10.1126/science.1250092;
RG International Wheat Genome Sequencing Consortium,;
RA Marcussen T., Sandve S.R., Heier L., Spannagl M., Pfeifer M.,
RA Jakobsen K.S., Wulff B.B., Steuernagel B., Mayer K.F., Olsen O.A.;
RT "Ancient hybridizations among the ancestral genomes of bread wheat.";
RL Science 345:1250092-1250092(2014).
RN [2] {ECO:0000313|Proteomes:UP000015105}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. AL8/78 {ECO:0000313|Proteomes:UP000015105};
RX PubMed=29158546; DOI=10.1038/s41477-017-0067-8;
RA Zhao G., Zou C., Li K., Wang K., Li T., Gao L., Zhang X., Wang H., Yang Z.,
RA Liu X., Jiang W., Mao L., Kong X., Jiao Y., Jia J.;
RT "The Aegilops tauschii genome reveals multiple impacts of transposons.";
RL Nat. Plants 3:946-955(2017).
RN [3] {ECO:0000313|EnsemblPlants:AET5Gv20304400.4}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2019) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00104}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EnsemblPlants; AET5Gv20304400.4; AET5Gv20304400.4; AET5Gv20304400.
DR Gramene; AET5Gv20304400.4; AET5Gv20304400.4; AET5Gv20304400.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000015105; Chromosome 5D.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd14327; UBA_atUPL1_2_like; 1.
DR Gene3D; 6.10.250.1630; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF398; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF06012; DUF908; 2.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
PE 3: Inferred from homology;
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000015105};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1314..1355
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 3312..3446
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 946..966
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1013..1053
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1805..1825
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2094..2226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2346..2612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2710..2740
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2945..2988
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3192..3222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1023..1040
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1807..1822
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2100..2150
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2151..2184
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2185..2199
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2200..2226
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2443..2471
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2473..2488
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2498..2515
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2526..2540
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2549..2577
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2594..2611
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3196..3220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3446 AA; 381153 MW; 409BAAD5CE2D8CA7 CRC64;
ADVLSSSVEN PEGEAQVRNP DRRWQRRRWQ RTGPASRCGC SRSCLAAAPC RRRSKSSPSR
PAKVKAFIDR VINIPLHDIA IPLSGFHWEF NKGNFHHWKP LFMHFDTYFK TYISSRKDLL
LSDDMSESEP LTKNTILQIL RVVQIVLENC QNKTTFAGLE HFKNLLASSD PEVVVAALET
LASVVKINPS KLHMNGKLIS CGAINSHLLS LAQGWGSKEE GLGLYSCVVA NERNQLEGLC
LFPADMENKY DGTQHRLGST LHFEYNLAPV QDSDQANDKS SNLCVIHMPD LHLRKEDDLS
ILKQCIDKFN VPPEHRFALF TRIRYAHAFN SPRTCRLYSR ISLLSFIVLV QSSDAHDELT
SFFTNEPEYI NELIRLVRSE DIVPGPIRAL AMLALGAQLA AYASSHERAR ILSGSSIISA
GGNRMVLLSV LQKAISSLSS PNDTSSPLIV DALLQFFLLH VLSSSSSGTT VRGSGMVPPL
LPLLQDKDPS HMHLVCLAVK TLQKLMEYSS PAVSLFKDLG GVELLSQRLH VEVQRVIGVA
EITSVLASDT SKSEDDHLYS QKRLIKALLK ALGSATYSPA NPARSQSSND NSLPMSLSLI
FQNVGKFGGD IYFSSVTVMS EIIHKDPTCF PALKELGLPD AFLSSVTAGV IPSCKALICV
PNGLGAICLN NQGLESVRET SVLRFLVETF TSRKYLIPMN EGVVLLANAV EELLRHVQSL
RSTGVDIIIE IINKLSCPRG DKITEAARAE EKTDMETDVE GRDLVSAMDS GTDGTNDEQF
SHLSIFHVMV LVHRTMENSE TCRLFVEKGG LQTLLTLLLR PTITQSSGGM PIALHSTMVF
KGFTQQHSTP LARAFCSSLK EHLKNALQEL DTVFRSCEVT KLEKGAIPSL FIVEFLLFLA
ASKDNRWMNA LLSEFGDVSR DVLEDIGRVH REVLWQISLF DEKKIEPEAS SPSANEAQQV
DAAVGDTDDN RYTSFRQYLD PLLRRRGSGW NIESQVSDLI NIYRDMGRAA TDSHRVGADR
YPSTGLPSSS QDQPSSSSDA NAKSEEDKKR SEHSSCCDMM RSLSYHINHL FMELGKAMLL
TSRRENSPIN LSPSVVSVAS NIASIVLEHL NFEGHTISPE REITVATKCR YLGKVVEFID
GILLDRPESC NPIMVNSFYC RGVIQAILTT FEATSELLFA MNRPPSSPME TDSKTGKEEK
DTDCSWIYGP LSSYGAAMDH LVTSSFILSS STRQLLEQPI FSGTVRFPQD AERFMKLLQS
KVLKTVLPIW AHPQFPECNL ELISSVTSIM RHVYSGVEVK NNVSNIAARL AGPPPDENAI
SLIIEMGFSR ARAEEALRQV GTNSVEIATD WLFSHPEEPP EDDELARALA MSLGNSDTPV
QEEDDRTNDL ELEEVNVQLP PMDEVLSSCL RLLQAKETLA FPVRDMLVTI SSQNDGQNRV
KVLTYLIDHL KQCLVASDPL KNTALSAFFH VLALILHGDT AAREVASKAG LVKVVLNLLC
SWELEPREGQ TTKVPNWVTS CFLSVDRMLQ LEPKLPDVTE LDVLKKDNSP TQTSVVIDDS
KKDSESSSSV GLLDLEDQEQ LLRICCKCIQ KQLPSGTMHA ILQLCATLTK VHVAAISFLE
SGGLHALLSL PTSSLFSGFN SVVSTIIRHI LEDPHTLQQA MELEIRHSLV TAANRHANPR
VTPRNFVQNL AFVVYRDPVI FMKAAQAVCQ IEMVGDRPYV VLLKDREKEK SKEKEKDKLV
DKDKSSGVAT KITSGDMVMA SPVSAKGKQS DLSARNMKSH RKPPQTFVTV IEHLLDLVMS
FVPPQRAEDQ SDGSSSMDMD IDSSSAKGKG KAVAVTHEES KQAIQDATAC LAKNAFVLKL
LTDVLLTYAS SVQVVLRHDA ELSSTRGPTR TSGGIFNHIL QHLLPHATKQ KKERKPDGDW
RYKLATRGNQ FLVASSIRSS EGRKRICSEI CSIFVEFTDN TGCKPPMLRM DAYVDLLNDI
LSARSPTGSS LSAESVVTFV EVGLVQCLTK TLQVLDLDHP DSAKIVTGIV KALEVVTKEH
VHLADFNAKG ENSSKTVLEQ NNVDSSSNRF QVLDTTSQPT AMVTDHRETF NAVHASRSSD
SVADEMDHDR DIDGGFARDG EDDFMHEIAE DRTGNESTMD IRFDIPRNRE DDMAEDEDDS
DEDMSGDDGE EVDEDDDDEE NNNLEEDDAH QRSHADTDQD DREIDEEEFD EDLLEEEDDD
DEDEEGVILR LEEGINGINV FDHIEVFGGS NNVSGDTLRV MPLDIFGTRR QGRSTSIYNL
LGRASDQGVL DHPLLEEPSM LLPQQRQPEN LVEMAFSDRN HENSSSRLDA IFRSLRSGRN
GHRFNMWLDD GPQRNGSAAP TVPEGIEELL LSQLRRPMAE HPDEQSTPAV DAQVNDPPSN
FHGPETDARE GSAEQNENNE NVDIPAVRSE VDGSASAGPA PPHSDELRRD ASNASEHVAD
MQYERSDTAV RDVEAVSQAS SGSGATLGES LRSLDVEIGS VEGHDDGDRH GASDRTPLGD
VQAATRSRRP SGNAVPVSSR DISLESVREI PPNTVQESDQ NASEGDQEPN RATGTDSIDP
TFLEALPEDL RAEVLSSRQN QVTQTSSEQP QHDADIDPEF LAALPPDIRE EVLAQQRAQR
LQQQSQELEG QPVEMDAVSI IATFPSEIRE EVLLTSPDTL LATLTPALVA EANMLRERFA
HRYHSGSLFG MNSRNRRGES SRRGDIIGSG LDRNTGDSSR QTASKLIETV GTPLVDKDAL
NALIRLLRVV QPIYKGQLQR LLLNLCAHRE SRKSLVQILL DMLMLDLQGS SKKSIDATEP
SFRLYGCHAN ITYSRPQSSD GVPPLVSRRV LETLTYLARN HPNVAKLLLF LQFPCPPTCH
TETLDQRRGK AVLVEDGEQQ SAFALVLLLT LLNQPLYMRS VAHLEQLLNL LEVVMLNAEN
EVNQAKLESS AERPSGPENA TQDALEDASV AGSSGVKPNA DDSGKSSANN ISDLQAVLHS
LPQAELRLLC SLLAHDGLSD NAYLLVAEVL KKIVALAPFI CCHFINELSR SMQNLTVCAM
NELHLYEDSE KAILSTSSAN GMAVLRVVQA LSSLVTSLQE RKDPELLAEK DHSDALSQIS
DINTALDALW LELSNCISKI ESSSEYTSNL SPTSANATRV STGVAPPLPA GTQNILPYIE
SFFVTCEKLR PGQPDAVQEP STSDMEDAST SSSGQKSSAS HTSLDEKHTA FVKFSEKHRR
LLNAFIRQNS GLLEKSFSLM LKVPRLIDFD NKRAYFRSKI KHQHDHHHSP VRISVRRAYI
LEDSYNQLRM RSPQDLKGRL TVHFQGEEGI DAGGLTREWY QLLSRVIFDK GALLFTTVGN
DLTFQPNPNS VYQTEHLSYF KFVGRVVGKA LFDAQLLDVH FTRSFYKHIL GAKVTYHDIE
AIDPAYYRNL KWMLENDISD VLDLTX
//
