ID A0A482IEV2_9CAUD Unreviewed; 846 AA.
AC A0A482IEV2;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
OS Synechococcus phage S-B68.
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes.
OX NCBI_TaxID=2545437 {ECO:0000313|EMBL:QBP06038.1, ECO:0000313|Proteomes:UP000294868};
RN [1] {ECO:0000313|EMBL:QBP06038.1, ECO:0000313|Proteomes:UP000294868}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Liu X.;
RT "Isolation and Genetic Analysis of a Novel Cyanophage S-LB68 from the Huang
RT Bohai.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755}.
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DR EMBL; MK016664; QBP06038.1; -; Genomic_DNA.
DR Proteomes; UP000294868; Genome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1280.300; -; 1.
DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1.
DR Gene3D; 3.40.1820.10; DnaQ-like 3'-5' exonuclease; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10322; DNA POLYMERASE CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR10322:SF23; DNA POLYMERASE DELTA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 2.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:QBP06038.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000294868};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:QBP06038.1};
KW Viral DNA replication {ECO:0000256|ARBA:ARBA00023109}.
FT DOMAIN 98..265
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 348..474
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 495..600
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
SQ SEQUENCE 846 AA; 98818 MW; AF785D928E8775B0 CRC64;
MRFYTYARLY GNSVLVRGWD DEKGGHFQER VPFRPTLFLP AKTETEWRTL DGYYVNPVEP
GTIKECRQFI DQYSGISGTK VYGMERFLYQ YLSKEYDENI EYDSTKIKMW SLDIETASEN
GFPKPELAEE EVLLITLKNF NSKKMITFGS RPWERTRDDV DYILCDSEYS LLESFVAWWE
TESPEIITGW NVDLFDMTYL CNRIQRVMGE KDQKRLSPWG LISSKELEHF GRTQQKYDIA
GVTILDYLDV YKKFTYTSRE SYRLDVIAEI ELGQKKLDHS EFETFKDFYT NGWNKFVDYN
LVDVDLVDRL EEKMKLIDLV MLMAYDAHVN YADCFAMVRL WDIIIYNDLR RKGIVLSAIE
RSDKKDQYAG AYVKEPIPGS YDWVVSFDLN SLYPSLIRFL NISPETLVDR KHPGANVEKM
IVKEADLSCE FDYCVAANGA QYRKDKVGFM PELVTRIYEE RKSYKKTMLQ KKQELVDIKE
ALKSDPKNED LLARKEQTIK EVTKWNNFQM ARKIQINSLY GALGNQYFRH YRLDNAEAIT
LTGQVSIRWI ERKVNEFCNK ALGTDGKDYV IASDTDSIYL NLGDLVSKAV PAGTSAEKTV
DILNQFCEDK IVPFIDRSYD ELSDYLNCME RTLVMKRECI AEKGIWTAKK RYILNVWDNE
GVRYNEPSLK MMGIEAVKSS TPSPCRTYIK ESLDIIMGGT EDQLIAYIEE KREEHSKLPP
EQVSFPRSCN NLNKFRDPVT LYRKSTPIHV RGSLLYNHRL KEMNLGHKYN QINDGEKIKF
AFLRKPNPIA ENVISFLGDL PKEFGLHDYL DYEMMFTKGF IDPLQAILEV IGWRTEKVAT
LEDFFG
//
