ID A0A493U2A7_ANAPP Unreviewed; 1010 AA.
AC A0A493U2A7;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE SubName: Full=Leucyl and cystinyl aminopeptidase {ECO:0000313|Ensembl:ENSAPLP00000032262.1};
GN Name=LNPEP {ECO:0000313|Ensembl:ENSAPLP00000032262.1};
OS Anas platyrhynchos platyrhynchos (Northern mallard).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Anseriformes; Anatidae;
OC Anatinae; Anas.
OX NCBI_TaxID=8840 {ECO:0000313|Ensembl:ENSAPLP00000032262.1, ECO:0000313|Proteomes:UP000016666};
RN [1] {ECO:0000313|Ensembl:ENSAPLP00000032262.1, ECO:0000313|Proteomes:UP000016666}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Hou Z.-C., Zhou Z.-K., Zhu F., Hou S.-S.;
RT "A new Pekin duck reference genome.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSAPLP00000032262.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
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DR AlphaFoldDB; A0A493U2A7; -.
DR Ensembl; ENSAPLT00000019492.1; ENSAPLP00000032262.1; ENSAPLG00000014098.2.
DR GeneTree; ENSGT00940000157902; -.
DR OMA; ERFFLSM; -.
DR Proteomes; UP000016666; Chromosome Z.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533:SF42; LEUCYL-CYSTINYL AMINOPEPTIDASE; 1.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000016666};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 159..344
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 378..596
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 675..993
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT ACT_SITE 451
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 450
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 454
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 473
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 535
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 1010 AA; 115486 MW; 578A22EE0A476A40 CRC64;
MIENSMFEEE PDVVDLAKEP CLHPLEPDEV EYEPRSSRLL VRGLGEHEMD DDEEDYESSA
KLLGMSFINR SSGLRNNMAG YRQSPDGSCS VPSTRTMVVC AAVFVTVISV IMVIYLLPKC
TFTKEGCHKK NRTMELIYPL ATNGKLFPWA KMRLPSDVVP LHYDLELQPN LTTLKFSGSV
KIVVNVLQVT RKIVLHSSGL NITKASLTST GGRQAKPVEL LEYPLHDQIA IIAPEALLVG
QNYTVNIEYS ANLSDTYYGF YKISYKDEDS KQRWFAATQF EPLAARSAFP CFDEPALKAT
FLIKIKRDEK LTALSNMPKK ATTPVTNGIV QDEFSVSLKM STYLVAFVVA DLKNISRETN
GSLVSVYAIP QHINQVEYAL NTTVKLLEFY QKYFLINYPL EKLDLVAIPD FQSGAMENWG
LVTFRETTLL YDSKTSSARD KKLITAVIAH ELAHQWFGNL VTMEWWNDLW LNEGFATFME
YFAMEEVFPE LHSDEDFLNL IFKAMMKDSM NSSHPVSSAV QSSEQIEEMF DTLSYIKGAS
LLLMLKNYLT EDVFQAGIGV YLHNHDYRSA QSDDLWDSMN EITNGTLDVK KLMKTWILNK
GFPLVTVVRS GRNISIQQEK FLYRVEPENW TSDASYLWHI PLTYMTNRCN FTHCVNAYLL
DQKSAVIELP EEVEWIKFNV DMNGYYIVHY AEDWKTLIDL LKKNHTALSA KDRANLINNI
FNLASLGKES LENAFELIDY LKEESSTAPL TQALFQLSRI YNLLEKKGEQ QLAARVMYRI
DNLLGNKIDQ QHWTDNGTLS ERELRSILLA FACTHDIRQC RTAATGMFEK WMKSEGTTSL
PSDLMKAIFI TGAKTNEGWE FLLKMYSSSV SEAEKSKMIE ALASTEDVRK LIWLMHNSLE
GEVIRAQELS HIVATVSQSL PGYLLAWDFV KENWEKLTRK FHLGSYTIQN LISSSTYQFA
TRAHLLEVKT FFESKSEESS KLRCVKEALD TIQLNIQWME RNLAKLQKWL
//
