ID A0A494VZ35_9SPHN Unreviewed; 724 AA.
AC A0A494VZ35;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE SubName: Full=3-hydroxyacyl-CoA dehydrogenase {ECO:0000313|EMBL:BBD97131.1};
GN ORFNames=SAMIE_1006320 {ECO:0000313|EMBL:BBD97131.1};
OS Sphingobium amiense.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=135719 {ECO:0000313|EMBL:BBD97131.1, ECO:0000313|Proteomes:UP000279959};
RN [1] {ECO:0000313|EMBL:BBD97131.1, ECO:0000313|Proteomes:UP000279959}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16289 {ECO:0000313|EMBL:BBD97131.1,
RC ECO:0000313|Proteomes:UP000279959};
RA Ootsuka M., Nishizawa T., Ohta H.;
RT "Complete Genome Sequence of the Nonylphenol-Degrading Bacterium
RT Sphingobium amiense DSM 16289T.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
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DR EMBL; AP018664; BBD97131.1; -; Genomic_DNA.
DR RefSeq; WP_066698950.1; NZ_AP018664.1.
DR AlphaFoldDB; A0A494VZ35; -.
DR KEGG; sami:SAMIE_1006320; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000279959; Chromosome.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000279959}.
FT DOMAIN 325..501
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 504..600
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 724 AA; 77328 MW; 647D66492C928184 CRC64;
MKTIRFDIDA DGIATLVIDV PGQSMNVIGP DLLADLDTAI TRIGSEDSIK GAVIASGKDS
GFMAGMDLKY FGSMLATGNG ERPAPADIFD RVFVLNRLLR RLETCGKPVA CAIEGTCVGG
GFELALACHR RFVGDSSKTQ LGLPEILIGL FPGGGGSQRL PRVMGVQAAL MYMLQGKLFR
PAEAAMLKVV DAVVPQGTAL DAAREWVKAN PTASTQPWDV KGFKVPGGAG GFNPAFVQTM
AGALPMTLKQ TQRNMNAPIA LLSAVYEGIT LPMDRAIQIE SKYFAKVAAD PQASNMIRTL
FVNKQAAERG ARRPKDEAKA PTRKLAMLGA GMMGAGIATV AAQAGMEVVL FDRDLAYAEK
GKAHVEEVLK KRLGKSMTPE KMAETLARVT PTTDYAALAG SDFVIEAVFE DVAIKAEVTK
QVEAVLGADT IFGSNTSTLP ITKLAQAWSR PENFIGIHFF SPVEKMPLVE IILGEKTGPA
AIAKALDFVA QIRKTPIVVH DSRGFYTSRS FGTYVQEGAE MVGEGINPAL IENAGKQLGM
PTGPLAVSDE VSIELGVKIM TAAKKELGDA YVPQASDDVA LKMVEAGRLG RKNGKGWYDY
PEGGKKHLSP QIGEMFPRAL HQPDVEAVKE RLLYRQLIEC ARCFAEGVLE TPEDGDIGAI
FGWGFAPYTG GPFSHMDTVG IAQVVETLDR LAREHGPRFA PTQQLRDMAA SGATFYGKVS
ARAA
//
