ID A0A495IWP1_9SPHI Unreviewed; 1116 AA.
AC A0A495IWP1;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=BDD43_1301 {ECO:0000313|EMBL:RKR81157.1};
OS Mucilaginibacter gracilis.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Mucilaginibacter.
OX NCBI_TaxID=423350 {ECO:0000313|EMBL:RKR81157.1, ECO:0000313|Proteomes:UP000268007};
RN [1] {ECO:0000313|EMBL:RKR81157.1, ECO:0000313|Proteomes:UP000268007}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18602 {ECO:0000313|EMBL:RKR81157.1,
RC ECO:0000313|Proteomes:UP000268007};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKR81157.1}.
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DR EMBL; RBKU01000001; RKR81157.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A495IWP1; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000268007; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000268007}.
FT DOMAIN 575..736
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 757..911
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1116 AA; 127290 MW; 72FAF480851516AC CRC64;
MNIREILERY KADERIQTLA TALNGAKNPR IQLRGLVGSS DAAMAIALYF LKHSHMLFIL
PDREEAGYFQ SDLESLLDKE ILLFPSSYRK PFEFTQPDSS NVLARAEVLN ELNHTSEYGQ
LIVTYPEALA EKVIDRASLE KNTLEIAVGN KLGLDFINEF LIEYDFERVD FVYSPGQFSI
RGGIVDIFSF SHDLPYRVEF FGDFIESIRT FEIESQLSVE QVKTITIVPN VQSKFLTESN
ISLLEYIDPS TQIWIRDVQF TLDIVQTGFK KATALWKALS AEEKNANPDW IDPKFAFTDE
KMIADQLHDF SLIEFGKQFF YTPTSSIQFD IRPQPSFNKD FSLLIHNFKN NEAEKVENCI
FTDSSRQLER LYAIFEDLDK TIKFTPVNIS IREGFIDHAQ KIACYTDHQI FDRYYKYKLK
KGYQRSQAIT LKELRELKPG DYITHIDHGI GKYAGLEKVE VGGKMQEMIR LLYADNDLLY
VNINSLNRIS KYSGKEGTVP KMNKLGTDTW EKLKKTTKKK VKDIARDLIK LYAVRKAQSG
NAFSPDSYLQ TELEASFIYE DTPDQEKATI DFKKDMESPH PMDRLICGDV GFGKTEVAIR
AAFKAVADSK QVAVLVPTTI LAAQHYKTFT ERLKGFPCNI DYVNRFKTSK QIKDTLQNLA
DGKLDIIIGT HRLVSKDVKF KDLGLMIIDE EQKFGVSTKE KLKAMRINVD TLTLTATPIP
RTLHFSLMGA RDLSIISTPP PNRQPVVTEL HVFNDKLIKE AVEFEIDRNG QLFFIHNRVA
DLPQLGGMIK KLVPKARIGI AHGQLEGDDL EDVMLKFVNN DYDVLVATTI IEAGLDIPNA
NTIIINYAHM FGLSDLHQMR GRVGRSNKKA YCYLLSPPLS TLTSEARKRL SAIEEFSDLG
SGFNVAMRDL DIRGSGNLLG AEQSGFIAEI GFEMYHKILD EAIQELKDDE FKGLFDDKPR
PFVSFTQIDT DLEILIPDEY VTSIPERYNL YTELSKLENE TELAAFAQQL HDRFGPVPPQ
VHDLLNTMRL QWLGKAIGFE KLSIKKNVLR GYFITNQQSP YFETAQFRQV LNFMQANPRR
CNMKEVKSTL RISIENVRGI DEAVAILEEM AEPVGV
//
