ID A0A495J232_9SPHI Unreviewed; 324 AA.
AC A0A495J232;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN ORFNames=BDD43_2958 {ECO:0000313|EMBL:RKR82772.1};
OS Mucilaginibacter gracilis.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Mucilaginibacter.
OX NCBI_TaxID=423350 {ECO:0000313|EMBL:RKR82772.1, ECO:0000313|Proteomes:UP000268007};
RN [1] {ECO:0000313|EMBL:RKR82772.1, ECO:0000313|Proteomes:UP000268007}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18602 {ECO:0000313|EMBL:RKR82772.1,
RC ECO:0000313|Proteomes:UP000268007};
RA Goeker M.;
RT "Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase II
RT (KMG-II): from individual species to whole genera.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKR82772.1}.
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DR EMBL; RBKU01000001; RKR82772.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A495J232; -.
DR OrthoDB; 629252at2; -.
DR Proteomes; UP000268007; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR Gene3D; 3.30.230.10; -; 1.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:RKR82772.1};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000268007};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 43..61
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 183..311
FT /note="DNA topoisomerase type IIA subunit B"
FT /evidence="ECO:0000259|Pfam:PF00204"
SQ SEQUENCE 324 AA; 37323 MW; DEF4D0B168FEA27C CRC64;
MDQRQKTFER SVRERPHMYL GNDGVIGLFA GLITDYIELY NTSNIFFTIT ILGANIFSFN
IQLKERLVMP KETSRVSANA IFFVQVLKVI SKKLEVQNGV MNQTEFNFNL DSDINLDLTA
DYQKLCEEIS LLAMLNTQIE IFVKDLRLNY TSQNYYNFPQ GIFYWFDRLN NEASGKPRFK
VTFEGMAQGF HYRIGLAYRT DWYPSPVILS FANNITTTRG GDLVDGVLSG LMSASRQFLK
DNGSTTFNIK RKKLYNGLIL VCAVKGKDLT YNGSFKEILV SKEVKAQAKE IVKKLITDLF
WQNKDVAESF LFRFDKQQIP SDIY
//
