ID A0A495JEL7_9ACTN Unreviewed; 1177 AA.
AC A0A495JEL7;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=BDK92_1444 {ECO:0000313|EMBL:RKR87171.1};
OS Micromonospora pisi.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=589240 {ECO:0000313|EMBL:RKR87171.1, ECO:0000313|Proteomes:UP000277671};
RN [1] {ECO:0000313|EMBL:RKR87171.1, ECO:0000313|Proteomes:UP000277671}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45175 {ECO:0000313|EMBL:RKR87171.1,
RC ECO:0000313|Proteomes:UP000277671};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKR87171.1}.
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DR EMBL; RBKT01000001; RKR87171.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A495JEL7; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000277671; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000277671};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..73
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1177 AA; 129459 MW; 509785377C448CEB CRC64;
MSDSFVHLHT HTEYSMLDGA ARLKELFAET NRLGMPALAM TDHGNLYGAY DFHKQATAAG
VKPIIGLEAY LTPGTDRRDR TRVRWADGGE NDVSGGGAYT HMTMLAQDAD GLRNLFALGS
RSSLEGYFYK PRADRELLNQ YGKGIIATTG CPSGEVQTWL RIGNFEKACE SAAEFRDIFG
ADNFYLELMD HGLDIETRIR QDLIRLGKTL NLKPIATNDL HYTHAADADA HEVLLCVQSG
STMADPKRFR FDARDFYLKS PAEMRAIWDA EVPGACDNTL EIAEKIGDYS VMFASRNLMP
QFPVPEGETE ETFLRKEVMR GLARRFPNGV PEDRQRQAEY ELDVILKMGF PGYFLVTADL
IAYAKREGIR VGPGRGSAAG ALIAYALGIT ELDPMQHGLL FERFLNPDRI SMPDIDMDFD
ERRRGDMIRY ATEKYGEARV AQIITYGTIK AKAAIKDAAR VLGYPFAVGD RITKAMPPAV
MGKDIPLGGI FDKNHPRYQE AAEFRSLYDS DAEVAKIVDT AKGLEGLKRQ WGVHAAGVIL
SRDPLLDVLP IHKREQDGSI ITQWDMGACE TIGLLKMDFL GLRNLTIMDD CLESIKENRG
IDLVLEDLPL DDAATYALLG RGDTLGVFQF DGGPMRALLR SMAPDNFEDI SAVGALYRPG
PMGANAHNEY ADRKNKRKPV VPIHPELAES LAEILGDTYG LIVYQEQVMA IAQKVAGYTL
GAADLLRRAM GKKKKEILDK EFKPFAAGMK ANGYSDEAIK TLWDILVPFS DYAFNKAHSA
AYGVVSYWTA YLKANYPAEY MGALLTSVGD DKDKSAVYLA ECRRMGIKVL PPDVNESNAR
FAAVGEDIRF GLAAVRNVGA NVVESIRRTR KEKGRFTDFY DYLRKVDAVA CNKKTIESLI
KAGAFDSLGH TRKGLLAVHT DAIDSFLDVK KKEATGQYDL FGTAFESEAS AVPGISVTPT
IPTTEWDKTD LLTFEREMLG LYVSDHPLFG VEHVLAAAAD MSIASLAEEG NVADGQIVNL
AGILSGVQRR ITKQGRAWAS ATLEDLGGAV EVLFFPNTYE VVGQYIAEDA IVVIKGRVDR
RDDQPRLMAM DMSMPEITAA DEVKPVVLAL PPSRCTPPLV ERLREVLTSH PGSAEVHVRL
VNGSRGTLLR LGRMRVAPST ALMADLKALL GPSAVAG
//
