ID A0A495KLB3_9FLAO Unreviewed; 1515 AA.
AC A0A495KLB3;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 13-SEP-2023, entry version 15.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=C8C84_1097 {ECO:0000313|EMBL:RKS01439.1};
OS Flavobacterium sp. 102.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=2135623 {ECO:0000313|EMBL:RKS01439.1, ECO:0000313|Proteomes:UP000270484};
RN [1] {ECO:0000313|EMBL:RKS01439.1, ECO:0000313|Proteomes:UP000270484}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=102 {ECO:0000313|EMBL:RKS01439.1,
RC ECO:0000313|Proteomes:UP000270484};
RA Chistoserdova L.;
RT "Synthetic bacterial communities of pure cultures isolated from sediment of
RT Lake Washington, USA.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKS01439.1}.
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DR EMBL; RBKX01000001; RKS01439.1; -; Genomic_DNA.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000270484; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd06127; DEDDh; 1.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..192
FT /note="Exonuclease"
FT /evidence="ECO:0000259|SMART:SM00479"
FT DOMAIN 262..350
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1515 AA; 172255 MW; C3A147644BFD9285 CRC64;
MYLIFDTETT GLPKRWGAPI SDTDNWPRCI QIAWQLHDAM GNLIEHQDYL VKPEGFNIPY
DAERIHGIST ELAQEQGIPL AVVLEKFNIA LAKAKFIVGQ NVGFDVNIMG CEFYRLGVNS
PMASMPVLDT CTEVTASLLK LPGGRGGKFK LPTLTELHSY LFNVPFAEAH NATADVEATT
RCFLELIRRE IFTKEELDVP TDYFREFGEN NPKEIQLIGL RHINLKQASE EIRKQFQKQE
EVVVPTNDKK QIHEALADVD FVHLHNHTQF SVLQSTISVK DLVAAAVQNK MPAVAMTDHA
NLMGAFHFVR DILYHNKSAQ AKNKQAEENG ETPTETIVKP IVGCEFYVCD DLKDKSRKDN
GYQIVFLAKT KKGYHNLAKL SSIAYTEGFY YVPRIDRKTI QQYKEDIIVL SGNLYGEIPS
KVLNIGENQA EEALIWWKQE FGDDFYIELM RHNQEDENRV NVSLIALAKK HQVKTVATNN
VFYINKEDAN AHDILLCVRD GEKQSTPIGR GRGYRYGLNN KEYYFKSGDE MKKLFHDLPE
SIASTAEIVN KIEIYDLSRE VLLPKFDIPS AFLVAEDEAD KGKRGENKYL HHLTYEGAKR
RYAEITPEIQ ERIDFELLTI ENSGYPGYFL IVQDFIAEAR KMGVSVGPGR GSAAGSVVAY
CLGITNIDPL LYNLLFERFL NPDRVSLPDI DIDFDDEGRS RVMDYVINKY GSKQVAQIIT
YGTMATKSSI RDTARVMDLP LFEADKVAKL IPGMMPSKWN LARLLSEDEA AIKKVLRPEE
FDKVRELISL SKENTLCSET INQAKFLEGN LRNTGIHACG VIITPSDITN FVPVATAKDS
DLYVTQFDNS VVESAGLLKM DFLGLKTLTL IKDTVKLVKY RTGKELNPDE FPIDDAKTYE
LFQRGETVGI FQYESAGMQK YMKELKPTVF GDLIAMNALY RPGPIAYIPS FIKRKNGEEP
IEYDLEACEE LLSDTYGITV YQEQVMLLSQ KLAGFSKGDA DVLRKAMGKK QKDVLDKMKP
KFIAQAEANG HPAEKLEKIW KDWEAFAEYA FNKSHSTCYA WIAYQTAYLK ANYPAEYMAA
VLSNNMNDIK QVSFFMEECK RMGLQVLGPD VNESFYKFTV NESYAVRFGM GAVKGVGANA
VDTIVQNRKE EKYKSIFDLA KKIDLRAANK KAFESLALAG GFDCFENTHR AQYFHIEGEG
ITFLEKAVRY GSKFQENENS SQVSLFGEAS EVQIAEPIVP PCDEWSTMEK LAKEKEVVGI
YISGHPLDDF KFEMKYFCNS KLEHLKNLNN YIGKNLTFAG IVTNVQYKTA KNGKDWAMFT
LEGYDESHDF RIFDEEYLKF RHFLVNNQFV YFKVGVKDGW VNRETGNKSE PRLQFLDVKQ
LQDVLSQYAK KLSIQMDIND LQQSLIQKLN EIFRSNEGDK SVNFEVIETE IVAKPVEIVK
STAPILMADT DSEIDIENLD SNEIVEEEMV QTAPVITEEI RRITTLEMPS RRLKIKISNE
LLLELEKMNV KFSLN
//
