ID A0A495XLN6_9PSEU Unreviewed; 4219 AA.
AC A0A495XLN6;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=Acyl transferase domain-containing protein {ECO:0000313|EMBL:RKT74802.1};
GN ORFNames=DFJ66_8173 {ECO:0000313|EMBL:RKT74802.1};
OS Saccharothrix variisporea.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharothrix.
OX NCBI_TaxID=543527 {ECO:0000313|EMBL:RKT74802.1, ECO:0000313|Proteomes:UP000272729};
RN [1] {ECO:0000313|EMBL:RKT74802.1, ECO:0000313|Proteomes:UP000272729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 43911 {ECO:0000313|EMBL:RKT74802.1,
RC ECO:0000313|Proteomes:UP000272729};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RKT74802.1}.
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DR EMBL; RBXR01000001; RKT74802.1; -; Genomic_DNA.
DR OrthoDB; 3653264at2; -.
DR Proteomes; UP000272729; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd08956; KR_3_FAS_SDR_x; 2.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 2.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF21089; PKS_DH_N; 2.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 2.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00826; PKS_DH; 2.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 4.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000272729};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:RKT74802.1}.
FT DOMAIN 5..417
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 885..960
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 974..1397
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2475..2550
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2563..2987
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4083..4158
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 4219 AA; 439860 MW; AFEBC5099F5ED2AD CRC64;
MTNLHDAVAV VGMSCRLPMA PDPAAFWRLL RDGRDAVVEL PPERREGLSP EVRGTRWGAV
LDGVGDFDAA FFGISPREAA ALDPQQRLVL ELAWEALEDA GIVPADLAGT PTSVFVGALR
DDYTALTLGR GAITQHTNIG THRGIIANRV SHALDLHGPS VVVDTAQSSS LVAVHLAAQS
VRSGESPLAL AAGVTLNLLA EGAVGAEKFG GLSPDGRCHT FDARANGYVR GEGAAVLVLK
PLRTALADGD EVHAVLLGSA VNNDGATPGL TVPDARAQER VVRAAQRRAG VTREQVQYVE
LHGTGTPVGD PVEAAALGAV FAGRELPLPV GSVKTNIGHL EGAAGVAGLL KVVLAIRNRQ
LPPSLHFSTP NPAIPLDELN LTVHTGLSGW PQPDRPLVAG VSSFGMGGTN CHVVVAEPAA
DPAQGSATAG GTRPAAQVVV PLSGRGVEAL RAQAARLRET AGEPVDLGWS LATTRTAFER
RAAVVATDRA SLDAGLAAIA AGAPGTGVVT GEVVEGAFGV VFTGQGAQRV GMGLGLHERF
PVFARAFDDA LAHFDPRLRD VIASGDGLDD TGWTQPALFA VEVAAHRLAE SWGLVPDVVL
GHSVGEIAAA HVAGVLSLAD AATLVSARGR LMAALPAGGA MVAVNAAEGD LDLPEDVDLA
AVNGPDSVVL SGAEGPTLAY AESLRGRGLR VKRLTVSHAF HSRLMDPMLD EFREVVAGLD
FRSPRIRVVS TVTGALAGDE LRDPEHWVRQ VRQPVRFLDA VRAADAVTLL EAGPDGVCSA
MAAAFGVRAF PMMRAGADEP TTALTALAAV HTRGVAVDWA GVYGGLDARR VALPTYPFQR
ERYWIAGQPG APATAPDTAA EKGGGAGQGL GMTGAGIGSV RRAAVDTAAL VAAHVAAVLG
VTDPGRVDLR RPFRDLGFDS LMGVELQDAL ADATGRSLPS GLLFDHPTPN AVVDYLSGAP
DPADEAAPDT TGGDEPIAIV GMACRYPGDV ASPEDLWGLV VGERDAIGPF PADRGWAPDL
HHPDPDRSGA SYVREGGFLG GAAEFDPAFF GISPREALAM DPQQRLLLQV AWEAVERAGI
DPVSLRGTRT GVFVGGTASD YGPRMHEASE QAEGHVLTGA TPSVMSGRIA YQLGLVGPAL
TVDTACSSSL VALHLAVRSL RSGESSAAIA GGVAVMATPG MFLEFSRQHG LAADARCKSF
SADADGTAWA EGVGLVVLER LADARRNGHR VLAVIRGSAV NSDGASNGLT APNGLSQQRV
IRQALADAGL STSDVDVVEA HGTGTSLGDP IEAEAILATY GQDRAEPLWL GSLKSNIGHA
QAAAGVGGVI KLVEAMRHGV LPRTLHVGEP TPRVDWGSGK VELLVRRREW PATDRPRRAA
VSSFGISGTN AHLVLEQGDV EPPVAPVAVT NPVAWVLSGH DERAVREQAT RLRALDAPDV
AAVARSLAGR TRFDHRAVVV GRTADELTAG LAAPDVVSAI PDARTAVLFT GQGAQRAGMG
RELHAAHPVF AAALDDVVAA FEPHLDRPLR DLMFGDHPEL HETRYTQPAL FAFEVALFRL
AEHHGLRADL LAGHSIGELV AAHVAGVLTL ADAAALVAAR GRLMQSARPG GAMVAVQARE
EDLDLPEGVS LAAVNGPESV VVAGDHDAVA AFAARFARTR RLAVSHAFHS HHMDDVLDEF
RAVAATVEHH EPTVPVVSTV DGRPLVADAD HWTRQIRATV RFLDAVHGLR DRGAGVFVEV
GPDAVLAPLA QSAGVTAVAL GRAGRPEVDV AVAGLARAHA HGATLDVASF APGDGRVDLP
TYPFRREVFW LSPRPRATGL VSDAVELAER DELVLTGEVS LGAQPWLAGH VVNGSVLVPA
TAFLELALAA GERVGLPRVD DLALEAPLVV PADGSVEVQV AVRDGRFTVH SRVDGWVRHA
SGTLAEAVGA VEEFPWPPDA EPEPVADAYD RLSALGYDYD GPFRGLTGMW RDGTTLYAEV
AVPAAERGFL VHPALLDAVL HPLVLAPAAA GEIRLPFAWS GVELRAADVT RLRVRITPTG
TDTYAIALAD GAGTPVGVIE GITLRAVAKD ALARDGGAAF ALEWRPVRTS GEPVGEVVVV
PPVDTDDPGV AVRRALGLVK DWLAAEHGPA DRLVFRTRRA VAARAGEPVD DLAGAAVWGL
VRVVQTEHPD QVVVVDAEHD DAVAAALATG EPQAAVRDGV VLVPRLVRAD AGDVTPGLGL
DPDGTVVVTG GTGGLGALVA RHLARRHGVT NLLLLSRRGL DAPGAAELAA ELGARVAAVD
VADRDALAAV LAGERITAVV HAAGVLDDAT TTALTDEQVD RVLAAKALAA RHLHELVGRV
DAFVLFSSVS GVLGTAGQAN YAAANTYLDA LAAHRRSLGL PATSLAWGLW AAGMGEGLGE
ADLARWARSG IAPLDAERGL ALFDRAVSTG PALLVPAALA PSRFDGDVVP APLRGLVRRR
PAAAPAPRAT ALTEASALEL VRATAAAALG HADARAVDPE RAFRDQGFDS LAGVDLRNRL
IAATGLRLPT TLVFDHPTPV ALAAHLVAGT GRKSAATTAV RADEPIAIVG MACRYPGGVR
SPQDLWRLVT EGRDAITGFP TNRGWDLDAL YDPDPDRLGT SYTREGGFLH DADLFDNAFF
GMSPREALAT DPQQRLLLET AWEAFEDAGI DPAALRGSRT GVFTGVMYDD YASRLPDTPD
EVEGFLLAGN TSSVISGRLA YTYGLEGPAI TVDTACSSSL VALHLAANAL RSGECDLALA
GGVTVMAGPS TFVEFSRQRG LARDGRCKSF SADADGTGWA EGVGLLLVER LSDARKRGHR
VLAVVRGSAV NSDGASNGLT APNGPSQERV IRQALAAARL STSDVDLVEA HGTGTTLGDP
IEAQAILATY GQDRAEPLWL GSLKSNIGHT QAAAGVGGVI KVVQAMRHGV LPRTLHAEHP
SPHVDWSSGA VELLVEPRAW PDTGRPRRAA VSSFGLSGTN AHVVLEQPDP EPDAERAPTA
GPVAWVLSAR DDDALRAQAE ALRAHLAEHP ELDVADVGLT LSRRALLERR AVVVGDREAL
ARGLADVRGG DGAGRGALAF LFTGQGSQRV GMGRDLYERS PVFRRALDEV CAHLDPLLDR
PLRTVLFAER DTPEEALLDQ TAFTQAALFA LEVALFRYVE HHGVRPDLLL GHSVGEVVAA
HVAGVLDLPD ACRLVAARGR AMQSAREGGA MAAIEATEEE VRAALRPGLA VAAVNAPRST
VVSGDEPAVV ATVREWRERG RRTRLLPVSH AFHSPHMDDV LAAFRDQLDG LVFRDPQITI
VSDVTGVVAT PEQLRSPDYW VTHVREAVRF LDGVRSLERL GATEFLELGP DGVLTALAAQ
CLTDEPGSVT PLLRAGRPES ESALAALGVL HTRGVRVTWD YPGARPVPLP GYPFRRRRFW
LDAPVAARGA DALGLGDTGH PLLGAAVELA GGDTVVLTGR VTAGSWLRDH RVGEDVLLPG
TALLDLALAA GARTGHPHVA ELTLAAPLVL GTARLQVTVG PAEAGRRPVT VHARAGDDVP
WELHASGVLA ADAPRPAAWE WPDDAEEVDL TGAYERLAGH GYHYGPAFQG LTSLRRRGDE
LFAEVHGPEQ AGFVVHPARL DAALHALLPG VVDEDAEAVV PFSWTGVTVH STTPAARVRL
TRRGSAVSLA VADAEGNPVL TVDELALRPL GRTAPPDGLH ALVWREMAVA DAPPHGRTVH
RVPADVRGRA AVHHVLEVLR GAEGPLAVVI RDGVDHAAVR GLVRSAQAEQ PGRFALATVA
DGTPDAELDA ALRLGEPEVA VRDGRVFVPR LVRSAGAGAD VAWGSGPVLI TGATGALGRV
LARHLVTAQG VRDLVLVGRR GPEAPGAEDL RAELAGLGAS VTLVACDVAD RDALAEVFAR
WRPTAVVHTA GVVADGTLDG LTTEQVDAVL RPKVDAAWNL HELAGDATAF VLYSSVAGLL
GTAGQANYAA GNAFLDALAE HRRAAGLPAV SLAWGLWETE SELSGGLSEV DLRRLGRSGL
RPLAADEAMR LFDAALAHGA PVLAVTRIDR AALRGRDDLP AVLRDLVRPA AAAPRPTAVE
TPADPEALVR HQVAAVLGHD DPDAVDPRRA FSELGFDSLT AVELRNRLRA ATGLDLPTTV
VFDHPSPAAL ADHVRGLLVA DEAGPVVDRL EALVRAGGLD DAALHRLRAL VGAAPAPDPD
LDLDAADDEA LFALVDELD
//
