ID   A0A498HR51_MALDO        Unreviewed;       878 AA.
AC   A0A498HR51;
DT   05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT   05-JUN-2019, sequence version 1.
DT   27-MAR-2024, entry version 12.
DE   RecName: Full=Glycosyl hydrolase family 13 catalytic domain-containing protein {ECO:0000259|SMART:SM00642};
DE   Flags: Fragment;
GN   ORFNames=DVH24_016768 {ECO:0000313|EMBL:RXH73946.1};
OS   Malus domestica (Apple) (Pyrus malus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX   NCBI_TaxID=3750 {ECO:0000313|EMBL:RXH73946.1, ECO:0000313|Proteomes:UP000290289};
RN   [1] {ECO:0000313|EMBL:RXH73946.1, ECO:0000313|Proteomes:UP000290289}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. HFTH1 {ECO:0000313|Proteomes:UP000290289};
RC   TISSUE=Young leaf {ECO:0000313|EMBL:RXH73946.1};
RA   Hu J.;
RT   "A high-quality apple genome assembly.";
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000256|ARBA:ARBA00004229}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RXH73946.1}.
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DR   EMBL; RDQH01000341; RXH73946.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A498HR51; -.
DR   STRING; 3750.A0A498HR51; -.
DR   Proteomes; UP000290289; Chromosome 15.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0043170; P:macromolecule metabolic process; IEA:UniProt.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR048650; ISOA1-3-like_C.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF3; ISOAMYLASE 3, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF21156; ISOA1-3_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW   Plastid {ECO:0000256|ARBA:ARBA00022640};
KW   Reference proteome {ECO:0000313|Proteomes:UP000290289};
KW   Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT   DOMAIN          353..771
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:RXH73946.1"
SQ   SEQUENCE   878 AA;  98991 MW;  E2469026CF738984 CRC64;
     CIPLLCRSPS LSSQPLSSSL SLFFHFPTSL PRDVASAGSI ETSLKWCPPF GPSFLRFIIF
     LGKFDPLLAL NILSQNFYRT LCNPTKRSRM LRTPHLYDSN VAKFPLFASP HSSHPVASCT
     LASPVLEMGL KWNKQASGSN QQGIVRKACL SFHNAGKSAF TRDGAKHSTA NAYGQRAQER
     VLEEEAPQLS ESSSSWKTFP GQAFPLGVSE VDSGINFAIF SQHATAVTLC LSLPERERLG
     GLDGGMMELP LDPNVNKTGD IWHICIKDLP RSNVLYGYRM DGPRGWHQGH RFDSSIVLVD
     PYAKLVEGRR MFGDTTKKLS KFLGTYDFDS LPFDWGENYK LPNIPEKDLV IYEMNVRAYT
     ADESSGLDPD IRGSYLGMIE KIPHLRELGI NAVELLPVFE FDEFEFQRRP NPRDHMINTW
     GYSTINFFAP MSRYASAGGG PVKASQEFKQ MVKALHGAGI EVILDVVYNH TNEADDANPY
     TTSFRGIDNK VYYMLDMNNN GQLLNYSGCG NTLNCNHPVV MELILDSLRH WVVEYHVDGF
     RFDLASVLCR GTDGTPLDAP PVIRAIAKDA ILSRCKIISE PWDCGGLYLV GRFPNWDRWA
     EWNGKYRDDL RRFIKGDSGM KGSFATRVSG SADLYKVNKR KPYHSVNFII AHDGFTLYDL
     VSYNFKHNEA NGEGGNDGSN DNLSWNCGHE GETDDSTIKA IRSRQMKNFH LALMISQGTP
     MMLMGDEYGH TRHGNNNSYG HDTALNHFQW GQLEARNNNH FRFFKEAIKF RQAHRVFGRE
     TFLEKHDVTW HEDNWDNYES KFLAFTFHDS SEGDIYLAFN AHDCFITVPI PAPPVKRRWF
     RVVDTNLESP NDIVPDGVPG IEGTYNMAPF SSVLLQAK
//