ID A0A498ITC5_MALDO Unreviewed; 615 AA.
AC A0A498ITC5;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase {ECO:0000256|ARBA:ARBA00039007};
DE EC=1.13.11.51 {ECO:0000256|ARBA:ARBA00039007};
GN ORFNames=DVH24_021792 {ECO:0000313|EMBL:RXH86519.1};
OS Malus domestica (Apple) (Pyrus malus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX NCBI_TaxID=3750 {ECO:0000313|EMBL:RXH86519.1, ECO:0000313|Proteomes:UP000290289};
RN [1] {ECO:0000313|EMBL:RXH86519.1, ECO:0000313|Proteomes:UP000290289}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. HFTH1 {ECO:0000313|Proteomes:UP000290289};
RC TISSUE=Young leaf {ECO:0000313|EMBL:RXH86519.1};
RA Hu J.;
RT "A high-quality apple genome assembly.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-
CC didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-
CC xanthoxin; Xref=Rhea:RHEA:19677, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:34596, ChEBI:CHEBI:35306;
CC EC=1.13.11.51; Evidence={ECO:0000256|ARBA:ARBA00036784};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-
CC dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin;
CC Xref=Rhea:RHEA:16541, ChEBI:CHEBI:15379, ChEBI:CHEBI:32304,
CC ChEBI:CHEBI:34597, ChEBI:CHEBI:35305; EC=1.13.11.51;
CC Evidence={ECO:0000256|ARBA:ARBA00035929};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a
CC 12'-apo-carotenal; Xref=Rhea:RHEA:23328, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:32304, ChEBI:CHEBI:51972, ChEBI:CHEBI:51973;
CC EC=1.13.11.51; Evidence={ECO:0000256|ARBA:ARBA00036011};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RXH86519.1}.
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DR EMBL; RDQH01000336; RXH86519.1; -; Genomic_DNA.
DR RefSeq; XP_008382970.1; XM_008384748.2.
DR AlphaFoldDB; A0A498ITC5; -.
DR STRING; 3750.A0A498ITC5; -.
DR EnsemblPlants; mRNA:MD10G0164700; CDS:MD10G0164700.1; MD10G0164700.
DR GeneID; 103445724; -.
DR Gramene; mRNA:MD10G0164700; CDS:MD10G0164700.1; MD10G0164700.
DR KEGG; mdm:103445724; -.
DR OrthoDB; 318119at2759; -.
DR Proteomes; UP000290289; Chromosome 10.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543:SF26; 9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED3, CHLOROPLASTIC; 1.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000290289}.
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 315
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 364
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 429
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 602
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 615 AA; 68329 MW; 6700C0A2F5820F1E CRC64;
MPSTATVAAT GTRVKPVTST TRHRRLSSLN SSNSFRNQLG FSPSSSSIFK SKTISCSLQT
PSLVQIPKHS PTYQRPSTST STKIPRENKT QKSPTSSKPV PQQWNLLQRA ASMALDAMES
ALLSKEKQHP LPKTSDPKVQ IAGNFAPVPE HPVEHYLPVT GKIPDSIQGV YVRNGANPLH
EPVAGHHLFD GDGMVHALQF KDGTASYACR FTETSRISQE RDLGRPLFPK AIGELHGHSG
IARLLLFYAR GMFGLVDPTH GIGVANAGLV YHNGHLLAMS EDDLPYHVQI TETGDLKTVG
RYDFEGQLKS TMIAHPKVDP ATGDLFALSY DVVQRPYLKY FKFSPNGEKS PDIEINLEQP
TMMHDFAITE RHVVIPDQQV VFKLQEMIGG GSPVIYDKNK MSRFGILDKN AKDASGIRWV
DCPDCFCFHL WNAWEEPETD EIVVIGSCMT PPDSIFNECD ENLESVLSEI RLNLKTGKST
RRRICSENLN LEAGMVNRNK LGRKTQSAYL ALAEPWPKVS GFAKVDLSTG EVKKHIYGDE
KYGGEPLFLP RDPNSEDEDD GYILAFVHDE KKWKSELQIV NTMDLKVEAT IQLPSRVPYG
FHGTFISAKD LKKQA
//
