ID A0A498K440_MALDO Unreviewed; 690 AA.
AC A0A498K440;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=EF-hand domain-containing protein {ECO:0000259|PROSITE:PS50222};
GN ORFNames=DVH24_003067 {ECO:0000313|EMBL:RXI02989.1};
OS Malus domestica (Apple) (Pyrus malus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX NCBI_TaxID=3750 {ECO:0000313|EMBL:RXI02989.1, ECO:0000313|Proteomes:UP000290289};
RN [1] {ECO:0000313|EMBL:RXI02989.1, ECO:0000313|Proteomes:UP000290289}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. HFTH1 {ECO:0000313|Proteomes:UP000290289};
RC TISSUE=Young leaf {ECO:0000313|EMBL:RXI02989.1};
RA Hu J.;
RT "A high-quality apple genome assembly.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Vacuole membrane
CC {ECO:0000256|ARBA:ARBA00004128}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004128}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RXI02989.1}.
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DR EMBL; RDQH01000329; RXI02989.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A498K440; -.
DR Proteomes; UP000290289; Chromosome 3.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0015368; F:calcium:monoatomic cation antiporter activity; IEA:UniProt.
DR CDD; cd00051; EFh; 1.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR004837; NaCa_Exmemb.
DR PANTHER; PTHR31503:SF79; CALCIUM-BINDING EF-HAND PROTEIN; 1.
DR PANTHER; PTHR31503; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR Pfam; PF13499; EF-hand_7; 2.
DR Pfam; PF01699; Na_Ca_ex; 1.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS00018; EF_HAND_1; 4.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 4: Predicted;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000290289};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Vacuole {ECO:0000256|ARBA:ARBA00022554}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..690
FT /note="EF-hand domain-containing protein"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5019770628"
FT TRANSMEM 154..180
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 253..275
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 541..562
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 574..592
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 628..655
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 661..683
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 315..350
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 355..390
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 428..463
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 468..503
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
SQ SEQUENCE 690 AA; 76702 MW; 21FD1F8DF1AE1C50 CRC64;
MSNITKTAYW VVFVLLVIMV EVRGRSFGLN TQGTSSSQLV SDGIDHEYQN ESTSSLLLLK
RIENRYSTES SCEQLYGFLP CSNSVFGHVF LMVVYEYLLF HGESYLAAGG EQIFKILGPG
VFGASAFHVI GALPESLLLL ASGFFNSKEI AQEYVYTGVG LLAGSSILSL TILWGTCVIV
SSQEFSNNLS SKASNDSNSS SFSWKRLSAS LTDWGTTTDS ETSSLARIMV CSVIPFLIMQ
MAKIFPSSSG ERIAILTALS VSVVFLLLYF IYQIFRPWVQ KRRLEFVKLG HLVSSILQHI
QKHALARVLT VKGAPNIPAI RRLFEEVDDD GDNYVSPSEV RELLCEIKFI STEDEKDKGT
EEVMKQFDLD HDGKINKDEF INGLDQVFQP WIINRRRERE IKKNLLSEVL RHVQINSLGS
IVTNDGRPDI PNIRRLFEKI DLDGNNFISQ SELRKLIGDN KFGKIPRNVD ESVVKLIEEL
DTSGDQLISE EEFVTGLTKW INKSNDQAPP SLESEDDIYQ EKTDKLVDEE KSSGGAIDES
IWAWVKAITY LVLGFVVLAV LAEPLIDSVQ NLSTAASIPS FLVAFVLVPL ATNARQATSA
IKAAAKKTPR TTSLTFSEIY GGVFMNNLLG FSVLLAIIYA LLVVLITCTV MGLIASFVST
LSLWTSFLAY LLYPISLLLV YILNDVVKYT
//
